Evaluation in vitro de l’activité antibactérienne de cinq plantes de la pharmacopée traditionnelle de l’Adamaoua (Cameroun)

Etchiké, Clément Agnem; Sassa, A. Mebanga; Abba, A. A.; Nyonbourg, Eugène

doi:10.4314/cajeb.v7i1.69788

Cited by 15 publications

(14 citation statements)

References 2 publications

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“…The aqueous extract of L. camara is very active on E. coli with a bactericidal effect and a MIC of 25 mg/mL. This strong activity is already demonstrated in more recent studies such as that of Etchike et al (16), which demonstrated the bactericidal effect on E. coli with a MIC of (1.47×10) − 2. The same observation was already made rather by Xaserra et Sharma (1999), but this time with the essential oils of the plant.…”

Section: Discussionmentioning

confidence: 49%

Exploration of the antibacterial and chemical potential of some Beninese pharmacopoiea traditional plants

et al. 2018

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SummaryObjectives. This study aims to evaluate the antibacterial and chemical properties of some medicinal plants used in the fight against enteropathogens in Benin.Methods. Aqueous and ethanolic extracts of Senna siamea, Uvaria chamae, Lantana camara and Phyllantus amarus were tested on 10 bacterial strains. Well diffusion technique, coupled with the microdilution determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (CMB) was used for antibacterial testing. The larval cytotoxicity was evaluated by using Artemia salina crustacean larvae. flavonoids and polyphenols were also assayed by the method using aluminum trichloride (AlCl3) and the method using the folin-Ciocalteu reagent, respectively.Results. The results of the study revealed that extracts had an effective antibacterial activity at 100 mg/mL, with MIC between 100 and 25 mg/mL and CMB between 100 and 50 mg/mL. The inhibition diameters of the extracts varied between 7.5 and 21 mm. The ethanolic extract of Phyllantus amarus leaves showed the best antibacterial activity. None of the extracts tested was found to be cytotoxic at the dose of 20 mg/mL. The aqueous Uvaria chamae root extract has the highest polyphenol content (231.896552±0.27586207 in μg EAG/100 mg extract), whereas the aqueous leaf extract of Uvaria chamae is the richest in flavonoids (41.061082 0.43180737 in μg ER/100 mg of extract).Conclusions. These interesting results can be used in the development of improved traditional medicines against enteropathogens. IntroductionInfections caused by enteropathogens are serious forms of infectious pathology. They are a major public health problem that causes millions of deaths a year. This is the case, for example, of foodborne illnesses, causing 17 million deaths a year worldwide, more than half of which come from the African continent (37). Diarrheal diseases are also the cause of 550 million patients each year, including 220 million children under 5 years of age (37). Salmonella, shigella and klebsielles are amongst others epidemiologically active enterobacteria involved in serious infectious diseases.Health management of enteropathogenic diseases is achieved through the use of antibiotics, but their inadequate and often anarchic use has resulted in bacterial resistance (24,36). Indeed, recent data from the bibliography abound with descriptions of bacteria that are multiresistant or even toto-resistant to antibiotics. The number of these bacteria is increasing in both industrialized and developing countries (32). This development of microbial resistance to antibiotics has led researchers to carry out alternative investigations to identify other effective natural remedies against various pathologies in the plant kingdom (18). It has therefore proved essential to look for new antibacterial substances that are effective and have a broad spectrum of action. One of the effective strategies for this research is to explore plants used in traditional Correspondence: Victorien T. Dougnon, Lecturer-Researcher in Microb...

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Section: Discussionmentioning

confidence: 49%

Exploration of the antibacterial and chemical potential of some Beninese pharmacopoiea traditional plants

et al. 2018

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“…3 Linear retention index taken from Adams [22] and/or NIST08 [23] for apolar columns, and from FFNSC2 [24] and/or NIST08 for polar columns. 4 Percentage values are means of three determinations with an RSD% for the main components below 5% in all cases. 5 Identification methods: std, based on comparison with authentic compounds; MS based on comparison with Wiley.…”

Section: Essential Oil Analysismentioning

confidence: 99%

“…(Asteraceae) is an herbaceous plant, 1.5 m in height, with pubescent, lanceolate leaves and flowers in yellowish panicles. In Cameroon, it is commonly found as a weed along roadsides, and it is widely used in folk medicine to treat angina, female infertility, AIDS, dental pain, headache and various diseases of microbial and non-microbial origin [1][2][3][4][5][6]. The leaf aqueous extract was proven to possess analgesic, anti-inflammatory and immunomodulatory activity [1,2], the dichloromethane extract inhibition against dermatophytes [7], and the leaf ethanolic and pentane extract antiplasmodial activity [8].…”

Section: Introductionmentioning

confidence: 99%

Biological Activities of the Essential Oil from Erigeron floribundus

et al. 2016

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Erigeron floribundus (Asteraceae) is an herbaceous plant widely used in Cameroonian traditional medicine to treat various diseases of microbial and non-microbial origin. In the present study, we evaluated the in vitro biological activities displayed by the essential oil obtained from the aerial parts of E. floribundus, namely the antioxidant, antimicrobial and antiproliferative activities. Moreover, we investigated the inhibitory effects of E. floribundus essential oil on nicotinate mononucleotide adenylyltransferase (NadD), a promising new target for developing novel antibiotics, and Trypanosoma brucei, the protozoan parasite responsible for Human African trypanosomiasis. The essential oil composition was dominated by spathulenol (12.2%), caryophyllene oxide (12.4%) and limonene (8.8%). The E. floribundus oil showed a good activity against Staphylococcus aureus (inhibition zone diameter, IZD of 14 mm, minimum inhibitory concentration, MIC of 512 µg/mL). Interestingly, it inhibited the NadD enzyme from S. aureus (IC 50 of 98 µg/mL), with no effects on mammalian orthologue enzymes. In addition, T. brucei proliferation was inhibited with IC 50 values of 33.5 µg/mL with the essential oil and 5.6 µg/mL with the active component limonene. The essential oil exhibited strong cytotoxicity on HCT 116 colon carcinoma cells with an IC 50 value of 14.89 µg/mL, and remarkable ferric reducing antioxidant power (tocopherol-equivalent antioxidant capacity, TEAC = 411.9 µmol·TE/g).

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“…The juice of the leaves is used to cure the wound (Bissangou and Ouamba, 1997). The previous studies undertaken in other countries give it some pharmacological properties such as: analgesic, anti-inflammatory and antipyretic (Owoyele et al, 2008), antibacterial (Etchike et al, 2011;Agban et al, 2013), anti-oxidative (Kavitha et al, 2013) and antifongic (Kra et al, 2009). This is the reason why anti-inflammatory and analgesic effects of leaves of C. odorata (Asteraceae) collected in Brazzaville-Congo in this study were investigated..…”

Section: Introductionmentioning

confidence: 99%

“…However, few medicinal plants were studied scientifically at the moment. In Africa, about 80% of the populations use them in the treatment of several pathologies (Etchike et al, 2011;Epa et al, 2015). In spite of the development of synthetic drugs, the vegetable drugs in his various forms occupy a special place.…”

Section: Introductionmentioning

confidence: 99%

Anti-inflammatory and analgesic effects of leaves of Chromolaena odorata L. (King and Robinson)

Itou¹,

Ossibi²,

Epa³

et al. 2017

Afr. J. Pharm. Pharmacol.

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This study was to evaluate anti-inflammatory and analgesic effects of the aqueous extract of leaves of Chromolaena odorata (Asteraceae) collected in Brazzaville-Congo. Acute inflammation was induced by using the carrageenan and formaldehyde models, and chronic inflammation by the cotton pellet induced granuloma model. Analgesic effect was evaluated by using the acetic acid-induced writhing, the pressure induced by the analgesymeter as well as the pain induced by formaldehyde. The results obtained show that aqueous extract (400 and 800 mg/kg) inhibits the edema induced by the carrageenan and formaldehyde. Moreover, this extract at the doses used (400 and 800 mg/kg) significantly inhibits granuloma fabric induced by cotton pellet. In addition, aqueous extract (400 and 800 mg/kg) inhibits significantly the pain induced by the three methods used. In conclusion, aqueous extract of C. odorata has anti-inflammatory and analgesic effects. These observations justify the traditional use of this plant in the treatment of inflammatory pathologies and the pain.

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Evaluation in vitro de l’activité antibactérienne de cinq plantes de la pharmacopée traditionnelle de l’Adamaoua (Cameroun)

Cited by 15 publications

References 2 publications

Exploration of the antibacterial and chemical potential of some Beninese pharmacopoiea traditional plants

Exploration of the antibacterial and chemical potential of some Beninese pharmacopoiea traditional plants

Biological Activities of the Essential Oil from Erigeron floribundus

Anti-inflammatory and analgesic effects of leaves of Chromolaena odorata L. (King and Robinson)

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