Evaluation of anti-obesity activities of ethanolic extract of Terminalia paniculata bark on high fat diet-induced obese rats

Abstract

BackgroundThe prevalence and severity of obesity and associated co-morbidities are rapidly increasing across the world. Natural products-based drug intervention has been proposed as one of the crucial strategies for management of obesity ailments. This study was designed to investigate the anti-obesity activities of ethanolic extract of Terminalia paniculata bark (TPEE) on high fat diet-induced obese rats.MethodsLC-MS/MS analysis was done for ethanolic extract of T. paniculata bark. Male Sprague–Dawley (SD) rats were randomly divided into six groups of six each, normal diet fed (NC), high fat diet-fed (HFD), HFD+ orlistat (standard drug control) administered, and remaining three groups were fed with HFD + TPEE in different doses (100,150 and 200 mg/kg b. wt). For induction of obesity rats were initially fed with HFD for 9 weeks, then, (TPEE) was supplemented along with HFD for 42 days. Changes in body weight, body composition, blood glucose, insulin, tissue and serum lipid profiles, atherogenic index, liver markers, and expression of adipogenesis-related genes such as leptin, adiponectin, FAS, PPARgamma, AMPK-1alpha and SREBP-1c, were studied in experimental rats. Also, histopathological examination of adipose tissue was carried out.ResultsSupplementation of TPEE reduced significantly (P < 0.05) body weight, total fat, fat percentage, atherogenic index, blood glucose, insulin, lipid profiles and liver markers in HFD-fed groups, in a dose-dependent manner. The expression of adipogenesis-related genes such as Leptin, FAS, PPARgamma, and SREBP-1c were down regulated while Adiponectin and AMPK-1alpha were up regulated in TPEE + HFD-fed rats. Furthermore, histopathological examination of adipose tissue revealed the alleviating effect of TPEE which is evident by reduced size of adipocytes.ConclusionsTogether, the biochemical, histological and molecular studies unambiguously demonstrate the potential anti adipogenic and anti obesity activities of TPEE promoting it as a formidable candidate to develop anti obesity drug.

Antiobesity Effects of Purple Perilla (Perilla frutescens var. acuta) on Adipocyte Differentiation and Mice Fed a High‐fat Diet

“…Leptin acts on the central nervous system to regulate appetite and energy metabolism (Yadav, Kataria, Saini, & Yadav, ). Obesity is characterized by increased leptin concentration and decreased levels of adiponectin in the blood (Mopuri, Ganjayi, Banavathy, Parim, & Meriga, ). Consistent with previous studies, PE supplementation reduced leptin levels and increased adiponectin levels, which may have ameliorated HFD‐induced changes in body weight and serum lipid parameters (Cui et al., ; Im et al., ).…”

Reduction of body weight, body fat mass, and serum leptin levels by addition of new beverage in normal diet of obese subjects

“…Reduction of serum leptin was also interrelated to satiety, and lessening in levels of serum leptin by the intake of beverage was determined to be linked with decreased food intake in hypercholesterolemic obese subjects. Similarly, reduction in serum leptin was also involved in regulating the SREBP‐1c gene expression, which is a transcription factor intricate in triggering carbohydrate and lipids metabolism by glucose regulation (Mopuri et al, ).…”

“…Snooping with lipid deposition has been suggested as an active way to cure obesity (Mopuri, Ganjayi, Banavathy, Parim, & Meriga, 2015). Dates vinegar and garlic juice were noted to decrease lipid deposition, so assisting to lessen body weight.…”

“…Similarly, reduction in serum leptin was also involved in regulating the SREBP-1c gene expression, which is a transcription factor intricate in triggering carbohydrate and lipids metabolism by glucose regulation (Mopuri et al, 2015).…”

Antiobesity and antihypercholesterolemic effects of white tea (Camellia sinensis) infusion on high-fat diet induced obese rats

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