Evaluation of Antibacterial Activities of Compounds Isolated From Sida rhombifolia Linn. (Malvaceae)

Woldeyes, Sileshi

doi:10.4172/2329-6836.1000101

Cited by 26 publications

(29 citation statements)

References 36 publications

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“…The biological activities of these compounds are listed in Table 2 [23][24][25][26][27][28][29][30][31][32][33][34] . Reports suggest that compounds 9,12,15-octadecatrienoic acid-2,3-dihydroxy propyl ester [26] , sitosterol [28,29] , squalene [30,31] and ethyl iso-allocholate [33] have antimicrobial activity. We selected these four compounds for docking studies to reveal the binding orientation of ligands with the target.…”

Section: Resultsmentioning

confidence: 99%

Ethyl Iso-allocholate from a Medicinal Rice Karungkavuni Inhibits Dihydropteroate Synthase in Escherichia coli: A Molecular Docking and Dynamics Study

Kullappan¹,

Anbarasu²,

Ramaiah³

2016

pharmaceutical-sciences

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Malathi, et al.: E. coli DHPS Inhibition by Ethyl IsoallocholateMedicinal rice varieties have defensive and therapeutic properties against many human disorders. Drug resistance has become a major problem in recent years. Escherichia coli have developed resistance to most of the antibiotics including sulfonamides that target dihydropteroate synthase. In the present study, we attempted to identify a novel inhibitor for the dihydropteroate synthase from a medicinal rice variety Karungkavuni. The phytochemical composition of the rice Karungkavuni was analysed through gas chromatography-mass spectrometry. The isolated compounds with reported antimicrobial activity were subjected to molecular docking procedures to understand the binding behaviour of the ligands with the target. These analyses revealed that ethyl iso-allocholate and 9,12,15-octadecatrienoic acid-2,3-dihydroxypropyl ester were the best binding compounds. Molecular dynamics studies revealed dihydropteroate synthase-ethyl iso-allocholate complex was more stable than other ligands throughout the simulation. Our study demonstrated that ethyl iso-allocholate isolated from Karungkavuni could serve as a potent inhibitor of dihydropteroate synthase.

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Section: Resultsmentioning

confidence: 99%

Ethyl Iso-allocholate from a Medicinal Rice Karungkavuni Inhibits Dihydropteroate Synthase in Escherichia coli: A Molecular Docking and Dynamics Study

Kullappan¹,

Anbarasu²,

Ramaiah³

2016

pharmaceutical-sciences

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“…The active dichloromethane extracts were subjected to column chromatography over silica gel and sephadex LH-20. As the result, eleven coumarins osthole (1) (Sajjadi et al, 2009), imperatorin (2) (Muller et al, 2004), bergapten (3) (Stevenson et al, 2003), prantschimgin (4) (Sajjadi et al, 2015), grandivitinol (5) (Abyshe et al, 1977), suberosin (6) (Tabanca et al, 2016), xanthotoxin (7) (Stevenson et al, 2003), felamidin (8) (Kilic et al, 2006), marmesin (9) (Abreu et al, 2010), umbelliferone (10) (Singh et al, 2010), ulopterol (11) (Doganca et al, 1979) and a sterol mixture consisted of stigmasterol (12), β-sitosterol (13) (Woldeyes et al, 2012) (Figure 1) were isolated and identified.…”

Section: Resultsmentioning

confidence: 99%

α-Amylase and α-glucosidase inhibitory activities of the extracts and constituents of Ferulago blancheana, F. pachyloba and F. trachycarpa roots

Karakaya

Özbek

Gözcü

et al. 2018

Bangladesh J Pharmacol

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Eleven coumarins named osthole (1), imperatorin (2), bergapten (3), prantschimgin (4), grandivitinol (5), suberosin (6), xanthotoxin (7), felamidin (8), marmesin (9), umbelliferone (10), ulopterol (11), and a sterol mixture consisted of stigmasterol (12), β-sitosterol (13) were isolated from the roots of Ferulago blancheana, F. pachyloba and F. trachycarpa through in vitro bioassay-guided fractionation processes. The extracts and bioactive compounds were evaluated for their α-amylase and α-glucosidase activities. Among the tested compounds, felamidin and suberosin showed significant α-glucosidase inhibitory activity with IC50 values of 0.4 and 0.9 mg/mL, respectively, when compared to the reference standard acarbose (IC50 = 4.9 mg/mL). Grandivitinol (IC50 = 20.0 mg/mL) had the lowest inhibitory effect. On the other hand, none of the tested extracts were found to be active on α-amylase inhibition. This is the first report on isolation, characterization of the bioactive compounds and evaluation the α-amylase and α-glucosidase inhibitory activities of these species.Video Clip of Methodology:8 min 26 sec: <a href="https://www.youtube.com/v/x0gakPjgXmc">Full Screen</a> <a href="https://www.youtube.com/watch?v=x0gakPjgXmc">Alternate</a>

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“…13 β-Sitosterol (3a) and stigmasterol (3b) were also reported to possess antibacterial activities. 14,15,16,17 …”

Section: Resultsmentioning

confidence: 99%

Chemical Constituents of Cymodocea rotundata Asch. and Schweinf

Perez¹,

Shen²,

Ragasa³

2018

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Introduction:Cymodocea rotundata Asch. and Schweinf, a widespread seagrass with reported antimicrobial activity, was investigated for its chemical constituents. Methods: The compounds were isolated by silica gel chromatography and identified by NMR spectroscopy. Results: This study has led to the isolation of β-sitosteryl-3β-glucopyranoside-6′-O-fatty acid esters (1), chlorophyll a (2) and a mixture of β-sitosterol (3a) and stigmasterol (3b) in about 1:1 ratio from the dichloromethane extract of C. rotundata. Conclusion: This is the first report on the isolation of 1-3b from C. rotundata. Compounds 2-3b were reported to exhibit antibacterial activity and may be partly responsible for the reported antimicrobial activity of the C. rotundata extract. Key words: Cymodocea rotundata, Cymodoceaceae, β-sitosteryl-3β-glucopyranoside-6′-Ofatty acid esters, Chlorophyll , β-sitosterol, Stigmasterol. Thin layer chromatography was performed with plastic backed plates coated with silica gel F 254 and the plates were visualized by spraying with vanillin/ H 2 SO 4 solution followed by warming. Sample CollectionSamples of the leaves of Cymodocea rotundata Asch. and Schweinf. were collected from the seagrass meadow of Caramoan, Camarines Sur Philippines in September 2016. The samples were authenticated at the Botany Division, Philippine National Museum. General Isolation ProcedureA glass column 6 inches in height and 0.25 inch internal diameter was used for the chromatography. The crude extracts were fractionated by silica gel chromatography using increasing proportions of acetone in CH 2 Cl 2 at 10% increment by volume as eluents. Five milliliter fractions were collected. All fractions were monitored by thin layer chromatography. Fractions with spots of the same Rf values were combined and rechromatographed in appropriate solvent systems until TLC pure isolates were obtained. Final purifications were conducted using Pasteur pipettes as columns. One milliliter fractions were collected. Isolation of the Chemical Constituents from the Leaves of C. rotundataThe air-dried C. rotundata (50 g) leaves were ground in a blender, soaked in CH 2 Cl 2 for 3 days and then filtered. The solvent was evaporated under vacuum to afford a crude extract (0.3 g) which was chromato- Ragasa et al.: Cymodocea rotundataPharmacognosy Journal, Vol 10, Issue 4, Jul-Aug, 2018 621 graphed using increasing proportions of acetone in CH 2 Cl 2 at 10% increment by volume. The 10% acetone in CH 2 Cl 2 fraction was rechromatographed using 10% EtOAc in petroleum ether. The less polar fractions were combined and rechromatographed using 10% EtOAc in petroleum ether to afford 2 (4 mg) after washing with petroleum ether, followed by Et 2 O. The more polar fractions were combined and rechromatographed (2 ×) using 15% EtOAc in petroleum ether to yield a mixture of 3a and 3b (6 mg) after washing with petroleum ether. The 60% acetone in CH 2 Cl 2 fraction was rechromatographed using CH 3 CN:Et 2 O:CH 2 Cl 2 (1:1:8, v/v) to afford 1 (3 mg) after washing with petrole...

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Evaluation of Antibacterial Activities of Compounds Isolated From Sida rhombifolia Linn. (Malvaceae)

Cited by 26 publications

References 36 publications

Ethyl Iso-allocholate from a Medicinal Rice Karungkavuni Inhibits Dihydropteroate Synthase in Escherichia coli: A Molecular Docking and Dynamics Study

Ethyl Iso-allocholate from a Medicinal Rice Karungkavuni Inhibits Dihydropteroate Synthase in Escherichia coli: A Molecular Docking and Dynamics Study

α-Amylase and α-glucosidase inhibitory activities of the extracts and constituents of Ferulago blancheana, F. pachyloba and F. trachycarpa roots

Chemical Constituents of Cymodocea rotundata Asch. and Schweinf

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