Evaluation of Antioxidant, Cytotoxic, Mutagenic and Other Inhibitory Potentials of Green Synthesized Chitosan Nanoparticles

Duraisamy, Narayanasamy; Dhayalan, Sangeetha; Shaik, Mohammed Rafi; Shaik, Althaf Hussain; Shaik, Jilani Purusottapatnam; Shaik, Baji

doi:10.3390/cryst12111540

Cited by 4 publications

(2 citation statements)

References 49 publications

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“…All measurements were made in triplicate to ensure accuracy and reliability. The percentage of DPPH radical scavenging was determined using the provided equation, 32 (2)…”

Section: Antioxidant Activity In Vitro Dpph Assaymentioning

confidence: 99%

Chemical Composition and Pharmacological Activities of Calamintha nepeta Essential Oil

2024

TJNPR

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Calamintha nepeta (Lamiaceae), widely distributed throughout the Mediterranean area, is a small and fragrant perennial herb traditionally used for both culinary and medicinal purposes. It serves as an antiseptic, spasmolytic, diuretic, and cough suppressant. The objective of this research is to characterise the chemical constituents of Calamintha nepeta essential oil (EO) through GC-MS analysis and evaluate its antioxidant and antimicrobial properties against nine multidrug resistant bacterial strains and one yeast. The study also conducted an in vitro anti-inflammatory 5lipoxygenase inhibition test and determined its lethal dose 50 (LD50) as well as the effects against carrageenan-induced paw edema in vivo. Analysis revealed that the primary components of C. nepeta included oxygenated monoterpenes, notably pulegone (58.36%), isoborneol (10.40%), menthone (8.91%), and piperitenone (3.86%). Antimicrobial tests demonstrated significant inhibition zones ranging from 10.2 to 37.12 mm. Essential oil exhibited minimum inhibitory concentrations and bactericidal/fungicidal concentrations ranging from 0.937 to 3.75 µL/mL and 0.937 to 15 µL/mL, respectively. Furthermore, it showed potent antioxidant activity against DPPH and a high total reducing power. Acute oral toxicity tests in Wistar rats indicated an LD50 of 2500 mg/kg. C. nepeta EO also exhibited strong anti-inflammatory properties against carrageenaninduced paw edema, with an IC50 value of 17.23 ± 0.32 μg/mL compared to diclofinac. Interestingly, this research highlights the importance of the phytochemical and antimicrobial properties of the essential oil of the Calamintha nepeta and accentuats its importance in pharmacological applications for treating inflammatory conditions.

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“…All measurements were made in triplicate to ensure accuracy and reliability. The percentage of DPPH radical scavenging was determined using the provided equation, 32 (2)…”

Section: Antioxidant Activity In Vitro Dpph Assaymentioning

confidence: 99%

Chemical Composition and Pharmacological Activities of Calamintha nepeta Essential Oil

2024

TJNPR

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“…The supernatant was removed, and the subsequent solution was centrifuged and the pellets were rinsed with acetic acid (to remove the unreacted part). After subsequent centrifugation, the chitosan nanoparticles (MA-CNPs) were freeze-dried [24].…”

Section: Synthesis Of Chitosan Nanoparticlesmentioning

confidence: 99%

Green Synthesis of Chitosan Nanoparticles Using of Martynia annua L. Ethanol Leaf Extract and Their Antibacterial Activity

et al. 2022

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The herbal-based drug isolation-related research has increased recently around the globe. Accordingly, the current study was designed to evaluate the phytochemical content of ethanol extract of Martynia annua and its chitosan nanoparticles (MA-CNPs) antibacterial activity against bacterial pathogens such as Bacteroides fragilis, Streptococcus oralis MTCC 2696, Propionibacterium acnes MTCC 1951, Pseudomonas aeruginosa MTCC 424, Staphylococcus aureus MTCC 2940, E. coli MTCC 443, Bacillus cereus MTCC 441, Streptococcus mutans MTCC 890, Aeromonas hydrophila MTCC 12301, and Streptococcus faecalis by agar well diffusion methods. The obtained results showed that the ethanol extract of M. annua contains more pharmaceutically valuable phytochemicals than other solvent extracts and its mediated chitosan nanoparticles showed effective antibacterial activities. The ethanol extract also effectively reduced, capped, and stabilized the chitosan into MA-CNPs. The green synthesized MA-CNPs were characterized and confirmed through UV-visible spectrophotometer, FT-IR, SEM, and DLS analyses. The MA-CNPs exhibited considerable antibacterial activity in the order of Bacteroides fragilis > Streptococcus oralis > Propionibacterium acnes > Pseudomonas aeruginosa > Staphylococcus aureus > E. coli > Bacillus cereus > Streptococcus mutans > Aeromonas hydrophila> Streptococcus faecalis. Finally, the results strongly recommended that the ethanol extract of M. annua-mediated chitosan nanoparticles could be considered an effective nanomaterial to control microbial pathogens. Further, therapeutical uses of MA-CNPs need in vitro and in vivo investigation.

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Carboxymethyl cellulose/starch-based films incorporating chitosan nanoparticles for multifunctional food packaging

Amaregouda,

Kamanna

2024

Cellulose

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Evaluation of Antioxidant, Cytotoxic, Mutagenic and Other Inhibitory Potentials of Green Synthesized Chitosan Nanoparticles

Cited by 4 publications

References 49 publications

Chemical Composition and Pharmacological Activities of Calamintha nepeta Essential Oil

Chemical Composition and Pharmacological Activities of Calamintha nepeta Essential Oil

Green Synthesis of Chitosan Nanoparticles Using of Martynia annua L. Ethanol Leaf Extract and Their Antibacterial Activity

Carboxymethyl cellulose/starch-based films incorporating chitosan nanoparticles for multifunctional food packaging

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