Evaluation of effect of alcoholic extract of heartwood of Pterocarpus marsupium on in vitro antioxidant, anti-glycation, sorbitol accumulation and inhibition of aldose reductase activity

Abstract

Rising popularity of phytomedicines in various diseased conditions have strengthened the significance of plant-research and evaluation of phytoextracts in clinical manifestations. Pterocarpus marsupium Roxb., a medicinal plant, known for its anti-oxidant and anti-diabetic activity is a rich source of phytochemicals with antihyperglycemic and antihyperlipidemic activities. However, its possible role in diabetic complications is not evaluated yet. The present study explores the possible role of alcoholic extract of heartwood of P. marsupium in the treatment of long-term diabetic complications. The alcoholic extract of P. marsupium was evaluated for advanced glycation-end-products formation, erythrocyte sorbitol accumulation and rat kidney aldose reductase enzyme inhibition at the concentration of 25–400 μg/ml using in-vitro bioassays. Also the phytoextract at the concentration of 10–320 μg/ml was evaluated for its antioxidant potential by in-vitro antioxidant assays which includes, determination of total phenol content; reducing power assay; nitric oxide scavenging activity; superoxide radical scavenging activity; total antioxidant capacity; total flavonoid content; DPPH scavenging activity; and hydrogen peroxide scavenging activity.The alcoholic extract of P. marsupium across varying concentrations showed inhibitory effect as evident by IC50 on advanced glycation-end-products formation (55.39 μg/ml), sorbitol accumulation (151.00 μg/ml) and rat kidney aldose reductase (195.88 μg/ml). The phytoextract also exhibited high phenolic and flavonoid contents with promising antioxidant potential against the antioxidant assays evaluated. The present investigation suggests that the phytoextract showed prominent antioxidant, antiglycation property and, inhibited accumulation of sorbitol and ALR enzyme, thus promising a beneficial role in reducing/delaying diabetic complications.

Diversity and biological activities of endophytic fungi associated with Catharanthus roseus

“…Post incubation period, 200 μL of Greiss reagent was added and the absorbance was read at 546 nm. The percentage of nitric oxide scavenging potential was calculated using the formula = (1-(Abs (546 nm) of the sample/ Abs (546 nm) of the control)) × 100 [40]. Ascorbic acid at a concentration of 50 μg mL − 1 was used as a positive control.…”

Phytoassisted synthesis of magnesium oxide nanoparticles from Pterocarpus marsupium rox.b heartwood extract and its biomedical applications

“…Based on the literature, synthesis of MgO-NPs with neem leaves [34], Parthenium [35], citrus lemon extract [36], Brassica oleracea, and Punica granatum peels [37] are available. Biological nanoparticles have been found safe, ecologically responsible, cost-effective, and ensures the complete removal of toxic chemicals [38]. Therefore, the green synthesis is still an unexplored area to achieve MgO-NPs, and it provides numerous research possibilities.…”

Effective anti-cancer property of Pouteria sapota leaf on breast cancer cell lines

“…Different parts of medicinal plants were investigated in order to find out its anti-cancer agents [2] . The anti-oxidants present in the medicinal plants was possibly responsible for the anticancer activity [3] . Likewise, P.sapota is a well-known fruit crop not majorly investigated for its medicinal and biological properties.…”