Evaluation of New Polyclonal Antibody Developed for Serological Diagnostics of Tomato Mosaic Virus

Mrkvová, Michaela; Hančinský, Richard; Grešíková, Simona; Kaňuková, Šarlota; Barilla, Ján; Glasa, Miroslav; Hauptvogel, Pavol; Kraic, Ján; Mihálik, Daniel

doi:10.3390/v14061331

Cited by 13 publications

(8 citation statements)

References 45 publications

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“…However, viral diseases have seriously affected tomato yield and quality [ 3 , 4 , 5 , 6 , 7 ], limiting global tomato trade [ 8 ]. The tomato mosaic virus (ToMV), a member of the genus Tobamovirus (family Virgaviridae ), is one of the most prevalent and devastating viruses affecting tomato production [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Genetic Variability and Molecular Evolution of Tomato Mosaic Virus Populations in Three Northern China Provinces

Lyu

Yang

Sun

et al. 2023

Viruses

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RNA viruses tend to mutate during transmission and host infection, which is critical to viral adaptation and evolution. Tomato mosaic virus (ToMV) is a member of the genus Tobamovirus (family Virgaviridae) and an economically important virus with detrimental effects on tomatoes worldwide. Although the ToMV gene sequences have been completed in China, their genetic diversity and population structure remain unclear. We collected 425 tomato samples from tomato-growing areas in three northern Chinese provinces 2016. Reverse transcription PCR results showed that the average incidence of the virus in the field samples was 67.15%, and ToMV was detected in all test areas. The analysis of ToMV single nucleotide polymorphisms in China showed that ToMV was evolutionarily conserved, and the variation in the whole genome was uneven. Pairwise identity analysis showed significant variability in genome sequences among ToMV strains with genomic nucleotide identities of 73.2–99.6%. The ToMV population in the northern Chinese provinces had purification and selection functions, which were beneficial in the evolution of the ToMV population. Although there has been some distribution of ToMV strains in China, the virus was generally stabilized as a uniform strain under the pressure of purification selection. Our findings show how to monitor the prevalent strains of ToMV and their virulence in China and provide useful information for its prevention and control.

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Section: Introductionmentioning

confidence: 99%

Genetic Variability and Molecular Evolution of Tomato Mosaic Virus Populations in Three Northern China Provinces

Lyu

Yang

Sun

et al. 2023

Viruses

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“…Five isolates of three tobamoviruses ( Table 1 ) were maintained alive in inoculated experimental plants. Virus presence in plants was tested by immunoblotting analysis using a polyclonal antibody developed for detection of tobamoviruses [ 41 ]. The symptomatic leaves from virus-positive plants were collected, sliced, mixed, and deep-frozen as a virus inoculum for subsequent experiments.…”

Section: Methodsmentioning

confidence: 99%

Elicitation of Hyoscyamine Production in Datura stramonium L. Plants Using Tobamoviruses

Mihálik

Hančinský

Kaňuková

et al. 2022

Plants

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Datura stramonium L. produces tropane alkaloids, and the hyoscyamine is dominant among them. Hyoscyamine is produced by hairy root cultures in vitro derived from native plants or plants with the genetically modified biosynthetic pathway for hyoscyamine. A common procedure is extraction from cultivated plants. Elicitors for increased production can be used in both cases. Live viruses are not well known for use as elicitors, therefore, D. stramonium plants grown in soil were artificially infected with the tobamoviruses Pepper mild mottle virus (PMMoV), Tomato mosaic virus (ToMV), and Tobacco mosaic virus (TMV). Differences in the content of hyoscyamine were between capsules and roots of infected and non-infected plants. Elicitation increased content of hyoscyamine in capsules 1.23–2.34 times, compared to the control. The most effective viruses were PMMoV and ToMV (isolate PV143), which increased content to above 19 mg/g of fresh weight of a capsule. The effect of each virus elicitor was expressed also in hyoscyamine content in roots. Elicited plants contained 5.41–16.54 times more hyoscyamine in roots compared to non-elicited plants. The most effective elicitor was ToMV SL-1, which raised production above 20 mg/g fresh weight of roots. It has been shown that tobamoviruses can be used as biotic elicitors.

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“…Tobamoviruses are very stable viruses [ 11 ]. It has been shown that the tobacco mosaic virus (TMV) can remain infectious for more than 50 years outside of the host [ 12 , 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Applicability of Different Methods for Quantifying Virucidal Efficacy Using MENNO Florades and Tomato Brown Rugose Fruit Virus as an Example

Zarghani

Ehlers

Monavari

et al. 2023

Plants

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After entry of a quarantine/regulated pathogen, infected plants shall be destroyed, and the cultivated area (e.g., greenhouse) shall be disinfected. Therefore, the selection of an effective disinfectant plays an important role. With the availability of different methods for virus quantification, we investigated the application of quantitative ELISA (qELISA), RT-qPCR (reverse transcription-quantitative polymerase chain reaction), and bioassays for the quantification of disinfectant efficacy. Therefore, we estimated the titer reduction in tomato brown rugose fruit virus (ToBRFV), a regulated pathogen, in plant sap and on germ carriers after treatment with MENNO Florades 4% for 16 h. The virus load before and after the treatment was measured with the mentioned methods. The RT-qPCR and qELISA methods showed very low efficacy in the presence of the disinfectant. Although bioassays are time-consuming, need purified particles for establishing the quantification models, and are less sensitive than RT-qPCR, they were able to quantify the differences in virus titer in the presence/absence of disinfectant. Interestingly, the bioassays reached at least the lower limit sensitivity of a qELISA test. By being less sensitive to the presence of the disinfectant, bioassays proved to be the only technique for the determination of the disinfectant efficacy against ToBRFV on different germ carriers as well as on virus-infected plant sap.

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Evaluation of New Polyclonal Antibody Developed for Serological Diagnostics of Tomato Mosaic Virus

Cited by 13 publications

References 45 publications

Genetic Variability and Molecular Evolution of Tomato Mosaic Virus Populations in Three Northern China Provinces

Genetic Variability and Molecular Evolution of Tomato Mosaic Virus Populations in Three Northern China Provinces

Elicitation of Hyoscyamine Production in Datura stramonium L. Plants Using Tobamoviruses

Applicability of Different Methods for Quantifying Virucidal Efficacy Using MENNO Florades and Tomato Brown Rugose Fruit Virus as an Example

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