Evaluation of Remineralization of Grape Seed Extract versus Sodium Fluoride on demineralized Primary Anterior Teeth: An In-vitro study

Amin, Riham A.; Awad, Samah; Sattar, Essam Abdel

doi:10.21608/edj.2015.71714

Cited by 8 publications

(5 citation statements)

References 11 publications

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“…Even though in the research procedure, after the dentin surface was applied, the MB stem extract was then cleaned and dried with cotton swab, not all of it disappeared, because it had reacted with the dentin surface to form a protective layer or physical barrier that made it more resistant to acid from outside. This is in line with the study of Amin et al 21 on SEM images showing a layer of precipitate of insoluble complexes on the enamel surface. The reaction product of the grape seed extract is seen as an amorphous lump.…”

Section: Discussionsupporting

confidence: 93%

“…Similar SEM images were also found in propolis-treated dentin which also showed that, after application of propolis to the dentin surface, a physical barrier was formed on the dentin surface, making it more resistant to acid. 5,21 Dentin consists of inorganic material (50%), organic components (30%), and water (20%). 22,23 In the present study, MB can inhibit the demineralization of tooth dentin's demineralisation based on C, O, P, and Ca value.…”

Section: Discussionmentioning

confidence: 99%

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Demineralized dentin characteristics after application of Mauli banana stem gel

Carabelly,

Puspitasari,

Syahrina

et al. 2024

Dent. J.

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Background: Demineralization of dentin is a condition of dissolving minerals in peritubular dentin due to exposure to acids that release hydroxyapatite ions. To prevent dentin demineralization, a therapeutic agent that can inhibit the dissolution ability of hydroxyapatite ions is needed. One therapeutic agent that can be used is the Mauli banana (MB) stem gel. Purpose: To observe the characteristics of dentin demineralization after the use of MB stem extract gel. Method: Mandibular incisor bovine teeth were demineralized with lactic acid pH 4.5 for 72 hours and then treated with 25%; 37.5%; 50%; and 62.5% MB gel and Chlorhexidine 2% for one minute. All samples were soaked in artificial saliva with 1 mg/ml saliva of collagenase enzyme for 24 hours. The characteristic of dentin demineralization was observed by using scanning electron microscope/electron dispersive X-ray spectroscopy (SEM-EDX). Results: The SEM image in the control, Chlorhexidine, 25%; 37.5%; 50%; and 62.5% MB gel groups showed dentinal tubules of about 3.67–4.94 µm; 3.55–4 µm; 4.18–5.6 µm; 2.28–2.86 µm; 3.29–3.81 µm; and 2.42–3.17 µm in size. The EDX test found carbon (C), nitrogen (N), oxygen (O), sodium (Na), phosphorous (P), chlorine (Cl), and calcium (Ca) in all groups. The one-way Analysis of Variance (ANOVA) test results showed significant differences in the levels of C, N, O, Cl, and Ca between all groups, while the Na showed no significant differences. Conclusion: The MB can inhibit the demineralization of bovine dentin based on the decrease in the size of the dentinal tubules and increasing the C, O, P, and Ca.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Demineralized dentin characteristics after application of Mauli banana stem gel

Carabelly,

Puspitasari,

Syahrina

et al. 2024

Dent. J.

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“…2016), who found that grape seed extract can increase the remineralization of carious enamel lesions. [24,22] They proposed that the GSE's proanthocyanidins (PAs) and gallic acid are responsible for facilitating mineral deposition on enamel. In addition, PACs have a chelating mechanism with calcium ions, promoting mineral deposition on the surface and that it could be an effective natural agent for non-invasive dentistry.…”

Section: Discussionmentioning

confidence: 99%

Remineralization Potential of Grape Seed Extract Versus Fluoride Mouthwash in Management of Post-Orthodontic White Spot Lesions: A 6m Randomized Clinical Trial

Abd El Sattar,

A.ElAziz,

Riad

2023

Advanced Dental Journal

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Aim:This study compared the remineralization potential of grape seed extract vs sodium fluoride mouthwash in adult patients with postorthodontic white spot lesions assessed with DIAGNOdent after six months. Materials and methods: A total of 14 participants with 40 lesions received mouthwashes randomly using either; grape seed extract 15% or sodium fluoride (1000 ppm), the participants were asked to use the mouthwash twice daily. The teeth were evaluated with DIAGNOdent after 1, 3 & 6 months. After 6 months, patient satisfaction was also recorded by using a binary questionnaire.Results: Regarding DIAGNOdent scores, 6 months intergroup comparisons revealed statistically significant differences (P=0.0198) for grape seed extract, and a statistically significant difference between different follow-up periods was found in intragroup comparisons within grape seed extract or sodium fluoride (P=0.0001). Regarding patient satisfaction, after 6 months, there was no statistically significant difference between the two materials (P = 0.5302). Conclusion: Both mouthwashes showed a potential remineralizing effect on post-orthodontic white spot lesions in the early testing periods, while grape seed extract mouthwash has a beneficial influence after 6 months. Clinical significance: Using grape seed extract as a natural remineralizing agent to treat early enamel carious lesions has the potential to be a viable approach.

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“…In addition, it has remineralizing properties through their catechin compound, which is a free radical scavenger needed for calcium absorption to enamel surface (17) . Also, spherical particles of calcium and phosphate were observed under scanning electron microscope deposited on enamel surface treated with GSE (18) . Moreover, in this study miswak increased dentin radiodensity, which coincide with previous study (19) .…”

Section: Discussionmentioning

confidence: 99%

The Anticariogenic Effect of Miswak (Silvadora Persica) and Grape Seed Extract

Khoriba¹,

Niazy²,

Sharkawy³

2022

ADJ-for Girls

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Purpose: To evaluate the effect of Miswak and Grape seed extract (GSE) on enamel and dentine subjected to artificial acid challenge in-vitro. Materials and Methods: A total of thirty sound freshly extracted sound human permanent periodontally affected upper molars were used. 30 enamel specimens were cut from the buccal surface of the teeth and 30 dentin specimens were cut from the cervical root area. All specimens were polished to obtain a flat polished surface. Ethanolic miswak extract was prepared by percolation method. Solutions of 10% ethanolic miswak extract, 6.5% Grape seed extract and 0.05% sodium fluoride were prepared. Specimens were divided into two groups (n=30); enamel and dentin. Each group was further divided into three sub-groups (n=10) according to the material used. Group1; treated with 10% ethanolic miswak extract solution. Group2; treated with 6.5% GSE solution. Group3; treated with 0.05% sodium fluoride solution (positive control). Specimens were individually subjected to alternative treatment/pH cycling regimen for 7 days. Radiodensity (change in mineral content) for each enamel and dentine specimen was assessed before and after pH cycling using digital radiography. Data were statistically analyzed; the significance level was set at p ≤0.05. Results: Miswak and GSE showed a high percent gain in enamel and dentine radiodensity compared to fluoride. Conclusion: Miswak and GSE are beneficial to both enamel and dentin through increasing their resistance to acid attacks.

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Evaluation of Remineralization of Grape Seed Extract versus Sodium Fluoride on demineralized Primary Anterior Teeth: An In-vitro study

Cited by 8 publications

References 11 publications

Demineralized dentin characteristics after application of Mauli banana stem gel

Demineralized dentin characteristics after application of Mauli banana stem gel

Remineralization Potential of Grape Seed Extract Versus Fluoride Mouthwash in Management of Post-Orthodontic White Spot Lesions: A 6m Randomized Clinical Trial

The Anticariogenic Effect of Miswak (Silvadora Persica) and Grape Seed Extract

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