Evaluation of serological test of Zika in an endemic area of flavivirus in the Colombian Caribbean

Abstract

The Zika virus (ZIKV) is an emerging flavivirus transmitted primarily through arthropods, endemic in Africa, Asia, and the Americas, and is considered a global threat by the World Health Organization. Objective To evaluate a commercial Zika virus test (IgG/IgM catalog number B815C, Biocan, Canada. Methods We evaluated 30 sera of patients diagnosed with Dengue, Leptospira, Malaria, Hantavirus, and Chikungunya. To establish the sensitivity of the test, two groups of sera were analyzed, the first one was patients with Zika RT-qPCR positive, and the second were patients RT-qPCR negative but with clinical suspicion of Zika. Results The specificity was of 23.3% (7/30), the sensitivity in acute patients with positive RT-qPCR was of 63.6%, the patients with clinical suspicion of Zika the sensitivity (IgM) was of 80% (n = 8/10). Overall sensitivity (IgM) of both groups was of 71.4% (15/21). Conclusions The test showed a low specificity to be used as a serological test in an endemic area of flavivirus infection.

Zika Virus Non-Structural Protein 1 Antigen-Capture Immunoassay

“…Other countries in the Americas also reported Zika cases during this time. Colombia reported over 100,000 suspected cases [ 16 ], with a total of 27 countries in the Americas reporting cases, including Mexico, Guatemala, the Dominican Republic and Panama [ 17 ]. Eventually, Zika cases were reported in the continental United States, with multiple introductions of ZIKV into Florida [ 18 ], as well as Texas.…”

Serological cross-reactivity using a SARS-CoV-2 ELISA test in acute Zika virus infection, Colombia

“…We investigated seroreactivity by using a commercial SARS-CoV-2 ELISA test in samples collected from groups from different individuals, including patients diagnosed to have Dengue, Zika, and Chikungunya virus infection, between 2015 and 2019, from the Córdoba Department, an endemic area for these arboviruses in the Caribbean Colombian region (Arrieta et al, 2019).…”

Registro de tecnologias durante epidemias: análise dos testes diagnósticos para vírus zika no Brasil

“…A cocirculação de outros arbovírus, especialmente DENV e CHIKV, no Brasil e na região das Américas requer o diagnóstico diferencial e preciso de zika. Isso é fundamental para a definição do acompanhamento clínico do paciente e para a implementação de programas de saúde pública, uma vez que os sintomas iniciais dessas doenças são semelhantes, mas sua evolução e seus efeitos são distintos (MARQUES et al, 2017;PEREIRA, 2018;ARRIETA et al, 2019).…”