Evaluation of the effects of transient or continuous PTH administration to odontoblast-like cells

Abstract

Parathyroid hormone participates in the metabolism of mineralized tissue. Its role in the formation of dentine is, as yet, incompletely understood. In the present study we analyzed the effect of transient (1 and 24-h/cycle) or continuous hPTH (1-34) treatment in odontoblast-like cells (MDPC-23) to the following parameters: mineral deposition detected by alizarin red, mRNA expression of the type I collagen (COL1), alkaline phosphatase (ALP), biglycan (BGN), matrix metalloproteinase 2 (MMP-2) and dentine sialophosphoprotein (DSPP) quantified by qRT-PCR. MMP-2 and ALP activities were quantified by zymography and colorimetric assay, respectively. The results showed that compared to Control group: intermittent PTH administration (1 and 24-h/cycle) decreased the mineral deposition and ALP activity. DSPP gene expression was not detected in both control and PTH treated cells. The PTH administration for 24-h/cycle increased the ALP, BGN and COL1 mRNA expression and continuous PTH treatment increased BGN and COL1 mRNA expression. Zymography assays showed that compared to Control group: PTH treatment for 1-h/cycle increased the total MMP-2 secretion and the continuous treatment decreased the secreted levels of MMP-2 active-form. Taken together, the results shown that PTH may regulate the odontoblast-like cells-induced secretion, and potentially this hormone can affect in vivo odontoblasts functions.

PTH/SDF‐1α cotherapy promotes proliferation, migration and osteogenic differentiation of human periodontal ligament stem cells

“…The addition of SDF‐1α in the culture media promoted the mRNA expression of osteogenic markers of BSP , OCN and Runx2 , and resulted in enhanced relative amount of calcium deposition, indicating that SDF‐1α enhanced osteoblastic differentiation of PDLSCs in vitro. PTH, as a major regulator of bone remodelling and calcium homoeostasis, stimulated osteoblastic bone formation not only in vitro but also in vivo . Tokunaga et al .…”

“…In this study, cells were cultured in the presence of 50 ng/mL PTH (Sigma‐Aldrich) and/or 200 ng/mL SDF‐1α (Santa Cruz Biotechnology, Santa Cruz, CA, USA) as experiment groups according to a previous study . Cells were maintained in PTH for 6 hours within a 48‐hour incubation cycle.…”

WISP-2 expression induced by Teriparatide treatment affects in vitro osteoblast differentiation and improves in vivo osteogenesis

“…It has been proven that the short administration of PTH (1-34) induces osteoblast hyperplasia, increases osteoblast survival and augments their ability to differentiate and to induce matrix mineralization. On the contrary, long term treatment with PTH (1-34), leads to the increment of osteoclast numbers and to the increase of their activity during bone resorption (Guimarães et al, 2013;Neer et al, 2001;Osagie-Clouard et al, 2017;Poole and Reeve, 2005;Sugiura et al, 2015). The molecular mechanisms occurring in osteocytes during treatment with PTH (1-34) are poorly known; reports published so far contradict each other, thus the activity of this hormone on osteocytes has not yet been elucidated as well as its ability to modulate some signaling pathways (Iida-Klein et al, 2005).…”

Self-Assembling Peptide P11-4 Presents Potential of Inducing Odontoblast-Like Cells to Biomineralization and Cell Migration

“…The medium was changed every 3 days and the mineral deposition was evaluated after 21 days of culture. Alizarin Red S staining protocol was followed to measure mineralization [22,23]. Briefly, after the culture period, the cells were washed with PBS and fixed with 4% paraformaldehyde for 1h at room temperature.…”