Evaluation of Wound Healing Properties of Grape Seed, Sesame, and Fenugreek Oils

Abstract

Background. Medicinal plants have proved at all times to be a powerful remedy for health care. Accordingly, grape seed, sesame, and fenugreek extracted oils with pharmacological properties are investigated as wound treatments. This study assesses the potential of our oils for healing wounds induced on rats. Methods. Phytochemical analyses of oils have involved: quality value, polyphenol, chlorophylls, carotene, and fatty acids. Antibacterial activity was carried out. Antioxidant activity was evaluated: the scavenging effect on DPPH radicals, the reducing power, and β-carotene discoloration. Uniform wound excision was induced on rats dorsum randomly divided into five groups: groups treated with “CICAFLORA®” and tested oils and untreated one. The posthealing biopsies were histologically assessed. Results. Wound biopsies treated with oils showed the best tissue regeneration compared to control groups. Groups treated with our oils and “CICAFLORA” had higher wound contraction percentage. Polyunsaturated fatty acids in oils act as inflammatory mediators increasing neovascularization, extracellular remodeling, migration, and cell differentiation. Wound healing effect was attributed to antibacterial and antioxidant synergy. Conclusion. According to findings, oils showed better activity in wound healing compared to “CICAFLORA” due to a phytoconstituents synergy. However, clinical trials on humans are necessary to confirm efficacy on human pathology.

Vegetable butters and oils in skin wound healing: Scientific evidence for new opportunities in dermatology

“…Its dried seeds contain from 10% to 16% grape oil (Fontanel, ; Janeš & Kočevar Glavač, ). In cold pressed grape oil, linoleic acid (polyunsaturated fatty acid) is the most abundant fatty acid in triglycerides, with a content ranging from 66% to 75%, followed by oleic (20%), palmitic (7%), and stearic (4%) acids (Garavaglia, Markoski, Oliveira, & Marcadenti, ; Janeš & Kočevar Glavač, ; Moalla Rekik et al, ).…”

“…A study using an excision wound model on rats showed the efficacy of grape oil to reduce wound size, increase the content of hydroxyproline in the granulation tissue, and promote the formation of a dense matrix of collagen fibres. Triglycerides or the respective fatty acids, as well as polyphenol compounds (191mg/kg; Moalla Rekik et al, ), may be responsible for wound healing. The results were significant compared with control groups (petrolatum and antibiotic mupirocin).…”

“…The groups treated with oils and CICAFLORA cream showed better wound contraction compared with untreated wounds. Polyunsaturated fatty acids in oils were assumed to act as inflammatory mediators and increase neovascularization, extracellular remodelling, and migration and cell differentiation, whereas the antibacterial and antioxidative activities of vegetable oils may contribute synergistic effects (Moalla Rekik et al, ).…”

Protective Effects of Unsaponifiable Matter from Perilla Seed Meal on UVB-induced Damages and the Underlying Mechanisms in Human Skin Fibroblasts

“…A recent study indicated that shea butter was a famous antioxidant and anti-inflammatory plant seed extract used in the cosmetic industry because of its high percentage of unsaponifiable compounds including tocopherols and phytosterols [13,14]. Rekik et al also reported that the positive effect of vitamin E and phytosterols on collagen synthesis and skin wound healing is because these compounds prevent the damaging effects of free radicals and ensure the stability and integrity of biological membranes [15]. With increasing interest in the development of functional materials with very few side effects, studies are being conducted to find various plant extracts that inhibit skin aging.…”

Physico-Chemical Analysis and Fatty Acid Profiling of Fenugreek (<i>Trigonella foenum-graecum</i>) Seed Oil Using Different Solvents

“…However, the main constituents of fenugreek oil are extremely useful in reducing free radicals 60 . It has been found that fenugreek seed oil is good source of nutraceticals like; α-tocopherol, ALA and sterols 62 as well as also a rich source of vitamin A 64 . Due to the presence of phytoconstituents fenugreek oil proved to have better activity on wound healing 64 and it is found best and safest treatment for male infertility 61 .…”