We consider a hub and spoke location problem (HSLP) with multiple scenarios. The HSLP consists of four subproblems: hub location, spoke location, spoke allocation, and customer allocation. Under multiple scenarios, we aim to provide a set of well-distributed solutions, close to the true Pareto optimal solutions, for decision makers. We present a novel multi-objective symbiotic evolutionary algorithm to solve the HSLP under multiple scenarios. The algorithm is modeled as a two-leveled structure, which we call the twoleveled multi-objective symbiotic evolutionary algorithm (TMSEA). In TMSEA, two main processes imitating symbiotic evolution and endosymbiotic evolution are introduced to promote the diversity and convergence of solutions. The evolutionary components suitable for each subproblem are defined. TMSEA is tested on a variety of test-bed problems and compared with existing multi-objective evolutionary algorithms. The experimental results show that TMSEA is promising in solution convergence and diversity.