Exogenously Applied Jasmonic Acid Induces Changes in Apical Meristem Morphology of Potato Stolons

Abstract

Hooked apex stolons and initial swelling stolons of potato plants were treated with 3 x 10-8 mol l-1 jasmonic acid (JA) to study the effect of this compound on histology, cell expansion and tissue differentiation. In hooked apex stolons, JA application increased the meristem thickness and reduced the length of the leaf primordia, whereas in initial swelling stolons narrowing of the apical region, absence of leaf primordia and swelling of the subapical meristem were evident. Early vascular tissue differentiation was observed in response to JA treatment, especially of xylem elements from regions proximal to the tunic. Protoxylem elements, such as tracheal elements, were present with thin primary cell walls. The cell area was measured in two zones: zone I, central mother cells situated immediately under the tunic; and zone II, rib meristem cells. JA caused a four- and six-fold increase in cell area in both zones in hooked apex stolons and initial swelling stolons, respectively. Thus, tuber formation is concluded to occur as a consequence of increased cell expansion, a reduction in the length of leaf primordia, enlargement of meristems, and early vascular tissue differentiation.

The Apoplastic Copper AMINE OXIDASE1 Mediates Jasmonic Acid-Induced Protoxylem Differentiation in Arabidopsis Roots

“…Interestingly, an earlier developmental expression pattern occurred after MeJA treatment, as shown by the appearance of GUS staining in a location closest to the root tip, suggesting a role of AtAO1 in MeJA signaling leading to protoxylem differentiation. In a few recent reports, a still quite unexplored role for MeJA in xylem development was suggested (Cenzano et al, 2003;Fattorini et al, 2009). In this regard, exogenous JA and MeJA have been shown to induce early xylem differentiation in potato stolons (Cenzano et al, 2003) and xylogenesis in tobacco thin cell layers (Fattorini et al, 2009), respectively.…”

“…In a few recent reports, a still quite unexplored role for MeJA in xylem development was suggested (Cenzano et al, 2003;Fattorini et al, 2009). In this regard, exogenous JA and MeJA have been shown to induce early xylem differentiation in potato stolons (Cenzano et al, 2003) and xylogenesis in tobacco thin cell layers (Fattorini et al, 2009), respectively. This work reveals that MeJA treatment results in early protoxylem differentiation in Arabidopsis wild-type seedlings ( Fig.…”

“…Of note, previous studies reported that the stress signaling hormone jasmonic acid (JA), while inducing root growth inhibition (Ren et al, 2009), behaves as a promoter of early vascular tissue differentiation (Cenzano et al, 2003) and xylogenesis (Fattorini et al, 2009). The role of JA in vascular tissue differentiation was first revealed in stolons of potato (Solanum tuberosum) during the tuberization process.…”

“…The role of JA in vascular tissue differentiation was first revealed in stolons of potato (Solanum tuberosum) during the tuberization process. In particular, exogenous JA accelerated potato tuber formation via the induction of both cell expansion and early differentiation of protoxylem vessels with ring-shaped secondary wall thickenings, leading to increased movement of nutrients toward the stolon tip (Cenzano et al, 2003). Moreover, exogenous methyl jasmonate (MeJA) was reported to enhance the formation of adventitious roots and the development of xylogenic nodules in tobacco (Nicotiana tabacum) thin layers under root-inductive hormonal conditions (Fattorini et al, 2009).…”

Uzun Gün Şartlarında Patates (Solanum tuberosum L.)’in Mikroçoğaltımında Jasmonik Asidin Etkisi

“…Previous reports suggests that high concentrations of JA (1.0 mg·L -1 ) resulting in an increase in the dry matter (%) of plantlets, whereas shorter plantlets with less branched shoots & roots of plantlets showed decreased fresh weight using a higher concentration (Zhang et al 2006). Takahashi et al (1994) and Cenzano et al (2003) suggested that the observed microtuber formation in the presence of JA could be due to increased cell expansion, a reduction in the length of leaf primordia, enlargement of meristems, and early vascular tissue differentiation. However, Vilhar et al (1997) suggested that JA supplementation altered morphology of roots through cell division and inhibited root elongation.…”