PurposeSome studies and reports have recently suggested using blockchain technology to improve transparency and trust in humanitarian supply chains (HSCs). However, evidence-based studies to display the utility and applicability of blockchains in HSCs are missing in the literature. This paper aims to investigate the key drivers and barriers of blockchain application to HSCs and explore whether evidence could support that the application of blockchain improves transparency and trust in HSCs.Design/methodology/approachThis paper puts forward a two-stage approach to explore the blockchain application in HSCs: an initial exploration of humanitarian practitioners and academicians interested in blockchain through focus group discussions; semi-structured interviews with practitioners involved at the UK Department for International Development's Humanitarian Supply Blockchain pilot project.FindingsFirst, we found that main drivers include accountability, visibility, traceability, trust, collaboration, time efficiency, reducing administrative work and cross-sector partnership. Main barriers, however, are composed of engagement issues, lack of technical skills and training, lack of resources, privacy concerns, regulatory problems, pilot scalability issues and governance challenges. Second, evidence from our case study revealed the blockchain application could have added value to improve visibility and traceability, thus contributing to improve transparency. Concerning trust, evidence supports that blockchain could enhance both commitment and swift trust in the pilot study.Practical implicationsOur study contributes to a more understanding of added values and challenges of blockchain application to HSCs and creates a perspective for humanitarian decision-makers.Originality/valueThis study provides the first evidence from the actual application of blockchain technology in HSCs. The study discovered that it is still less evident in many humanitarian organizations, including medium- and small-sized nongovernmental organizations, that they engage in a direct deployment of in-house or customized blockchain-based HSC. Instead, these actors are more likely to indirectly use blockchain in HSCs through a private commercial partner.