Expression of activation markers by human lung T cells after adherence to lung epithelial cells

Abstract

Both increased T cell numbers and their increased activation state have implicated an important role for T cells in chronic inflammatory reactions seen in the airways of (allergic) asthmatics. Airway epithelial cells are frequently exposed to stimuli that cause the release of mediators and the expression of cell adhesion molecules. We have examined whether human airway epithelial cells can activate lung-derived T cells. Clonal lung T cells showed an increased adherence to transformed airway epithelial cells that had been exposed previously for 2 h to human recombinant interferon-gamma (IFN-gamma; 100 U/ml). After an additional 16-24 h of culturing in the absence or presence of epithelial cells, T cells expressed increased levels of both the alpha-chain of the interleukin-2 receptor (IL-2R, CD25) and the transferrin receptor (CD71), both markers of T cell activation. T cells apparently activated by epithelial cells, however, did not produce IFN-gamma or IL-4 nor showed an increased proliferation on the addition of IL-2 (5-50 U/ml). The induced adherence to and the activation of T cells by epithelial cells is mediated largely by CD2 and its ligand lymphocyte functional antigen-3, a pathway known to up- and downregulate T cell functions.

Lung epithelial H292 cells induce differentiation of immature human HMC‐1 mast cells by interleukin‐6 and stem cell factor

“…H292, a human lung mucoepidermoid carcinoma‐derived cell line (ATCC CRL 1848, Rockville, Maryland, USA), was cultured in RPMI‐1640 (Gibco BRL) supplemented with 10% heat‐inactivated FCS, penicillin (100 U/mL) streptomycin (100 μg/mL), and 0.5 m m l ‐glutamine and propagated as described before [ 13].…”

IFN-γ Amplifies IL-6 and IL-8 Responses by Airway Epithelial-Like Cells Via Indoleamine 2,3-Dioxygenase

“…After incubation, cells were detached using trypsin/EDTA at room temperature (28). Cell surface expression of HLA-DR was evaluated using PE-anti-HLA-DR (BD Biosciences, Mountain View, CA) as compared with an isotype control (IgG2a), using a FACScan (BD Biosciences).…”

Mediators Produced by Airway Epithelial Cells Influence Proliferation and Differentiation of Immature Mast Cells

“…Mo., USA), penicillin (100 U/ml, Brocades Pharma, Delft, The Netherlands) and streptomycin (100 pg/ml, Gibco). 11292 cells, a human lung derived mucoepidermoid carcinoma cell line (American Type Culture Collection CRL 1848, Rockville, Md" USA) were cultur ed and propagated as described before [6], Medium was conditioned by H292 confluent monolayers for 48 It after which medium was clear ed from cell debris by centrifugation and stored at 20°C pending use.…”