Expression pattern and partial sequence analysis of a fetal bovine myosin heavy-chain gene

Abstract

A fragment of a bovine myosin heavy-chain (MHC) gene approximately 15 kbp in size (designated MHC 67) was isolated from a bovine genomic DNA library. The direction of transcription was determined, and preliminary experiments indicated that the gene was expressed in fetal skeletal muscle. The expression pattern of this gene was, therefore, evaluated in detail using northern blots containing RNA from eleven different bovine muscle and nonmuscle tissues at three developmental ages. A restriction fragment of clone MHC 67 containing the 3' untranslated sequence (which is specific for each MHC gene) was used as a probe. This gene fragment hybridized predominantly to RNA from fetal skeletal muscles and did not hybridize to RNA from either neonatal or adult skeletal muscles (red or white), smooth muscle tissue, or nonmuscle tissue. A 7-kb EcoRI fragment containing both translated and untranslated regions surrounding the 3' end of the gene was subcloned into pBluescript II KS+ and partially sequenced. When these bovine sequences were aligned to that of the human and rat skeletal and cardiac MHC genes, we found that these sequences corresponded to exons 31, 32, and 33, and that they had homology with human perinatal and fetal MHC as high as 90% at the nucleotide level and 97% at the amino acid level. Comparison of the nucleotide sequences of isoform-specific 3' nontranslated regions from bovine, human, and rat genes further verify that the MHC 67 clone encodes the bovine fetal or perinatal MHC isoform.

iTRAQ-Based Identification of Proteins Related to Muscle Growth in the Pacific Abalone, Haliotis discus hannai

“…Here, we identified several proteins involved in molluscan muscle growth, including the myosin II heavy chain (MYH II) and MYH; previous research has indicated that the myosin gene is positively correlated with muscle development [ 23 , 24 ]. The myosin heavy chain 67 was expressed in many bovine tissues, including the adductor muscle, the longissimus dorsi, the psoas, the atrium, the ventricle, the aorta, and the liver [ 25 ]. In the Siniperca chuatsi , a freshwater fish species, the highest level of MYH-7b protein expression is found in red muscle, followed by the skin, pituitary, and eyes, while the lowest levels are found in the myocardium, gills, and gonads [ 26 ].…”

Impact of periconceptional and preimplantation undernutrition on factors regulating myogenesis and protein synthesis in muscle of singleton and twin fetal sheep

“…1). In the perinatal period, skeletal muscle is composed of myofibers expressing the myosin, heavy chain 8, skeletal muscle, perinatal (MYH8) (Young et al 1994).…”