Extraction conditions of white rose petals for the inhibition of enzymes related to skin aging

Abstract

In order to assess inhibitory potentials of white rose petal extracts (WRPE) on the activities of enzymes related to dermal aging according to the extraction conditions, three extraction methods were adopted. WRPE was prepared by extracting dried white rose (Rosa hybrida) petals with 50% ethanol (WRPE-EtOH), Pectinex® SMASH XXL enzyme (WRPE-enzyme) or high temperature-high pressure (WRPE-HTHP). In the inhibition of matrix metalloproteinase-1, although the enzyme activity was fully inhibited by all 3 extracts at 100 µg/mL in 60 min, partial inhibition (50-70%) was achieved only by WRPE-EtOH and WRPE-enzyme at 50 µg/mL. High concentrations (≥250 µg/mL) of all 3 extracts markedly inhibited the elastase activity. However, at low concentrations (15.6-125 µg/mL), only WRPE-EtOH inhibited the enzyme activity. Notably, WRPE-EtOH was superior to WRPE-enzyme and WRPE-HTHP in the inhibition of tyrosinase. WRPE-EtOH significantly inhibited the enzyme activity from 31.2 µM, reaching 80% inhibition at 125 µM. In addition to its strong antioxidative activity, the ethanol extract of white rose petals was confirmed to be effective in inhibiting skin aging-related enzymes. Therefore, it is suggested that WRPE-EtOH could be a good candidate for the improvement of skin aging such as wrinkle formation and pigmentation.

The Ethanol Fraction of White Rose Petal Extract Abrogates Excitotoxicity-Induced Neuronal Damage In Vivo and In Vitro through Inhibition of Oxidative Stress and Proinflammation

“…In our previous studies, WRPE has been shown to improve allergic dermatitis, skin aging, and especially ischemic stroke via its antioxidant and anti-inflammatory properties [ 21 , 22 , 23 , 24 ]. Therefore, we extended the activity of WRPE to excitotoxic brain injury, in which oxidative and inflammatory processes play an important role.…”

“…Previously, we reported that white rose petal extract (WRPE) displayed a broad spectrum of antioxidant [ 21 ], anti-bacterial and anti-fungal [ 22 ], anti-allergic, and anti-inflammatory properties [ 23 ]. Notably, a butanol fraction of WRPE containing high amounts of gallic acid and volatile compounds reduced the infarction volume in an animal model of ischemic cerebral stroke [ 24 ].…”

In vitro screening of elastase, collagenase, hyaluronidase, and tyrosinase inhibitory and antioxidant activities of 22 halophyte plant extracts for novel cosmeceuticals

“…There were several previous studies mentioned about the splendid antioxidant activities by R. rugosa and the other results by Olech et al that revealed the effective hyaluronidase inhibition of R. rugosa extracts is probably due to its the richness in polyphenol content (Ng et al 2004;Olech et al 2017). Nonetheless, another finding suggested that the ethanol extraction of Rosa hybrida, a plant species belonging to the same family with R. rugosa, provided the anti-elastase and anti-tyrosinase activity as a candidate for the improvement of skin aging (Choi et al 2015).…”

Evaluation of antioxidant and anti-collagenase activity of Rosa damascena L. flower petal and receptacle extract

“…In the body, ROS promote the activation of collagenase and block collagen synthesis, leading to a decrease in skin elasticity and acceleration of skin wrinkles. This mechanism by ROS is the major factor of degeneration collagen fiber 6 .…”