Extraction of gelatin from catfish bone using NaOH and its utilization as a template on mesoporous silica alumina

Abstract

Gelatin extraction from catfish bone using NaOH and its utilization as a template on a synthesis of mesoporous silica-alumina had been investigated. The extraction was prepared by immersing 25 g catfish bone in 125 mL of NaOH in concentration of 0.0; 0.05; 0.10; 0.15 and 0.20 M for 24 h, then washing with demineralized water until pH 7, followed by immersed the bone into 125 mL of 1 M HCl for 1 h, then washed using demineralized water into pH 5. To produce gelatin the bone was refluxed with 100 mL demineralized water at 70C for 5 h then evaporated at 50C. The dry gelatin was characterized using FTIR and electrophoresis (SDS-PAGE). The best performance of gelatin was produced by NaOH 0.10 M. The gelatin consists of amide A, B, I, II, III and molecular weight of 25-200kDa. Silica and Alumina material prepared from Lapindo mud extraction. Dry Lapindo mud crushed and filtered until pass 100 mesh, then reflux using 6 M HCl (1:4 w/V) at 90C for 5h then filtered. The filtrate was consisting alumina solution adding with 6 M NaOH (2/3 V/V) them filtered. The filtrate then injected by CO2 gas for 30 minutes and filtered, the residue was calcined at 500C for 5h. The residual of Lapindo mud dried and refluxed with 6 M NaOH (1:4 w/v) at 90 C. After 5h filtered and the filtrate added by HCl to pH 8 and filtered, the residual then dried. The Si and Al were then analyzed by XRF and consist of silica and alumina for 99.1 and 87.73%, respectively. Silicaalumina was prepared using silica and alumina extracted from Lapindo mud. 6 g of SiO2 and 2 g of NaOH was immersed in 62 mL of demineralized water then added with alumina solution (0.204 g alumina in 30 mL demineralized water). The gelatin solution (5 g gelatin in 70 mL demineralized water) was dropped into the silica-alumina while stirring at 50C for 4 h and aging for 24 h. The synthesized silica alumina was analysed using FTIR and surface area analyser. The FT-IR spectra indicated the TO4 (T=Si, Al) vibration at wave number of 1049.28 and 1103.23 cm -1 . The synthesized silica-alumina showed mesoporous characters with a pore diameter of 41.18 nm and surface area of 32.76 m 2 /g

Production and Characterization of Edible Film from Catfish (Clarias gariepinus) Bone Gelatin Incorporated with Red Dragon fruit (Hylocereus Polyrhizus Britton and Rose) Peel Extract

“…This result indicated that catfish bone gelatin has several functional groups similar to commercial gelatin . This result was different from another research about gelatin catfish bone by Nuryanto 15 , that claimed catfish bone gelatin has functional group of C=O, C-N, =C-N, NH and CH 2 .…”

Production and characterization of gelatin from rabbit bone as bioplastics material by acid pre-treatment

“…Different sources of gelatin and different curing treatment resulted in different molecular weights of gelatin proteins. The iridescent shark fishbone treated with HCl and NaOH resulted in 116-200 kDa gelatin proteins (Mahmoodani et al, 2014), while the iridescent shark fishbone with citric acid pre-treatment resulted in 38-162 kDa gelatin proteins (Pertiwi et al, 2018), iridescent shark fishbone with pineapple waste treatment resulted in 75-225 kDa gelatin proteins (Atma et al, 2018), and iridescent shark fishbone with 0.1 M NaOH immersion resulted in 25-200 kDa gelatin proteins (Nuryanto et al, 2018).…”

Osteocranium Anatomy of African Catfish (Clarias gariepinus Burchell 1822) from Cultured Pond in Aceh, Indonesia

“…African catfish (Clarias gariepinus, Burchell 1822) is one of the important cultured fish species extensively consumed by Indonesian people (1). However, to date, studies related to African catfish in Indonesia are still limited to fish feed formulations (2,3), aquaculture techniques (4), genetic improvement (5), and processed products (6).…”