Extraction of phenolic compounds with antioxidant potential from coconut (Cocos nucifera L.) testa and identification of phenolic acids and flavonoids using UPLC coupled with TQD-MS/MS

Arivalagan, M.; Roy, Tapas Kumar; Yasmeen, Ammara; Pavithra, K. C.; Jwala, P.N.; Shivasankara, K.S.; Manikantan, M. R.; Hebbar, K. B.; Kanade, Santosh R.

doi:10.1016/j.lwt.2018.02.024

Cited by 70 publications

(66 citation statements)

References 36 publications

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“…Considering the significant effect of the extraction solvent on the antioxidants extracted from plants [ 19 , 20 , 21 ], twelve different types of solvents were used to select the suitable solvent to get the maximum extraction of antioxidants from wild rice, since they were the most common ones for the extraction of antioxidants from plants [ 7 , 13 , 19 , 22 ]. The antioxidant activities, total flavonoid content (TFC), and total phenolic content (TPC) of each solvent extract were determined ( Figure S1 in the Supplementary Materials ).…”

Section: Resultsmentioning

confidence: 99%

Partial Purification, Identification, and Quantitation of Antioxidants from Wild Rice (Zizania latifolia)

et al. 2018

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To provide further insights into the potential health-promoting antioxidants from wild rice (Zizania latifolia), which is an abundant but underutilized whole grain resource in East Asia, a partial purification based on D101 macroporous resin was carried out for the purification and enrichment of the antioxidants from the bioactive ethanol extracts of wild rice. On that basis, 34 phenolic compounds in the antioxidant fractions were identified by a high-performance liquid chromatography-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC-LTQ-Orbitrap-MSn). The results suggested that phenolic acids could be enriched in the 10% ethanol-eluted fraction whereas flavonoids (including procyanidins and flavonoid glycosides) could be enriched in 20–30% ethanol-eluted fractions. A quantitative analysis determined by the multiple reaction monitoring mode of the ultra-performance liquid chromatography-triple quadrupole-tandem mass spectrometry (UPLC-QqQ-MS/MS) revealed a high content of procyanidins in wild rice. Compared with phenolic acids, flavonoids may contribute more to the potent antioxidant activity of wild rice. This is the first study on the antioxidants from wild rice Z. latifolia. These findings provide novel information on the functional components of wild rice, and will be of value to further research and development on Z. latifolia.

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Section: Resultsmentioning

confidence: 99%

Partial Purification, Identification, and Quantitation of Antioxidants from Wild Rice (Zizania latifolia)

et al. 2018

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“…Compounds in EL-EA were identified by comparing the retention time, parent ion, and mass fragments with available standards in databases (ChemSpider, mzCloud, mzVault, HMDB, and PubChem) and/or literature data [ 9 , 10 , 11 , 12 , 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…The mobile phase B and the used gradient condition are shown in Table 3. Compounds in EL-EA were identified by comparing the retention time, parent ion, and mass fragments with available standards in databases (ChemSpider, mzCloud, mzVault, HMDB, and PubChem) and/or literature data [9][10][11][12][13][14][15][16][17][18][19][20][21].…”

Section: Uplc-esi-ms/ms Analysismentioning

confidence: 99%

In Vitro Antibacterial and Antiproliferative Potential of Echinops lanceolatus Mattf. (Asteraceae) and Identification of Potential Bioactive Compounds

Seukep

Zhang

et al. 2020

Pharmaceuticals

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Many species belonging to the genus Echinops are widely used in traditional medicine to treat infectious diseases and cancers. The present study aimed to evaluate the antibacterial and antiproliferative properties of Echinops lanceolatus Mattf. (Asteraceae). The activity of the methanolic extract and subsequent partition fractions was investigated against drug-resistant bacteria (Gram-negative and Gram-positive) and human tumor cell lines using broth microdilution and sulforhodamine B (SRB) assay, respectively. Our findings revealed weak to moderate antibacterial activities of tested extracts, with the recorded minimal inhibitory concentrations ranging from 256 to 1024 µg/mL. The ethyl acetate fraction (EL-EA) was found to be the most effective. Likewise, that fraction displayed strong antiproliferative potential with recorded IC50 of 8.27 µg/mL and 28.27 µg/mL on A549 and HeLa cells, respectively. An analysis based on the ultra-performance liquid chromatography–electrospray ionization tandem mass spectrometry (UPLC–ESI–MS/MS) of the EL-EA fraction allowed the identification of 32 compounds, of which quinic acid and derivatives, cinnamic acid derivatives, dihydrokaempferol, naringenin-7-O-glucoside, apigenin-7-O-d-glucoside, naringin, apigenin, rhoifolin, coniferyl aldehyde, and secoisolariciresinol are well-known compounds of biological importance. This study is first to report on the biological activity and phytochemical profile of E. lanceolatus. We provide a baseline to consider E. lanceolatus as a valuable source of anti-infective and antiproliferative agents.

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“…In previous studies have shown that flavonoids torbangun leaves less soluble in water. Several studies has been reported the best solvent extraction for flavonoids compound such as ethanol and acetone (Dirar et al, 2019), water-acetone (Alcântara et al, 2019), acidified solvents of water, acetone, ethanol, and methanol (Arivalagan et al, 2018). The response surface for α-glucosidase inhibition as a function the percentage mixtures of water, ethanol, and acetone is presented in Figure 7.…”

Section: Total Flavonoids Contentmentioning

confidence: 99%

Optimization of Total Flavonoids Extraction and Α-Glucosidase Inhibitory Activity from Plectranthus amboinicus (Lour.) Spreng. Leaves using the Simplex-Centroid Design

Wibisono

Aisyah

Suhesti

et al. 2019

Molekul

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The leaves of Plectranthus amboinicus (Lour.) Spreng. (Lamiaceae) is a perennial plant that contained flavonoids compounds with have antidiabetic properties. The aim of the work was to select the best solvent for extraction of the flavonoid compounds from torbangun leaves. A statistical simplex-centroid mixture design for the water, ethanol, and hexane solvents has been used to the extraction of extract yield, flavonoids content, and inhibitory α-glucosidase activity of torbangun leaves. The ethanol-water extracts showed the highest extract yield with value of 15.92%. The ethanol-hexane extracts presented the highest total flavonoids content, 96.30 mg QE/g, followed by hexane extract (82.91 mg QE/g). The ethanol extract recorded higher inhibitory of α-glucosidase activity, with value of 25.53%, followed by hexane extract (25.21%), and ethanol-water extract (23.02%). The weak correlation between the α-glucosidase inhibition and total flavonoids content showed that flavonoids contribute non-significantly to the α-glucosidase activity.

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Extraction of phenolic compounds with antioxidant potential from coconut (Cocos nucifera L.) testa and identification of phenolic acids and flavonoids using UPLC coupled with TQD-MS/MS

Cited by 70 publications

References 36 publications

Partial Purification, Identification, and Quantitation of Antioxidants from Wild Rice (Zizania latifolia)

Partial Purification, Identification, and Quantitation of Antioxidants from Wild Rice (Zizania latifolia)

In Vitro Antibacterial and Antiproliferative Potential of Echinops lanceolatus Mattf. (Asteraceae) and Identification of Potential Bioactive Compounds

Optimization of Total Flavonoids Extraction and Α-Glucosidase Inhibitory Activity from Plectranthus amboinicus (Lour.) Spreng. Leaves using the Simplex-Centroid Design

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