Extraction of poly-3-hydroxybutyrate using chlorinated solvents

Ramsay, J. O.; Berger, Esther; Voyer, Richard; Chavarie, C.; Ramsay, Bruce A.

doi:10.1007/bf00152152

Cited by 184 publications

(128 citation statements)

References 9 publications

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“…In fact, after thermolysis of the cell membrane, the granules of P(3HB) are released and solubilized, as described for other methods in the literature [32] Differently, chloroform is an aggressive chemical that does not need heating to cause cellular disruption [12] and this is why the P(3HB) standard extraction method can be performed at low temperatures. Nonetheless, it should be noted that the P(3HB) extraction requires, besides refrigeration at 4°C to preserve chain length [33], at least 36 h of contact time between chloroform and the bacterial cells.…”

Section: P(3hb) Extractionmentioning

confidence: 99%

“…The most routinely used P(3HB) solvents in laboratory scale are chlorinated organic compounds, namely chloroform and dichloromethane [10,17].However, large-scale application of these solvents causes environmental and health concern. Moreover, the process requires high volumes of solvent and anti-solvent for the precipitation [11], increasing the cost of extraction and subsequent solvent recovery.…”

Section: Introductionmentioning

confidence: 99%

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Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents

Rosengart

Cesário

Almeida

et al. 2015

Biochemical Engineering Journal

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A technique for poly-3-hydroxybutyrate (P(3HB)) extraction with safer, non-chlorinated solvents, was developed, aiming to attain high recovery yields and purities. A wide range of solvents was selected from the GlaxoSmithKline guide as sustainable industrial solvents and the solubility of P(3HB) on those solvents calculated using predictive equations from literature. Anisole, * Corresponding author: Tel.: +351 21 8419137; Fax: +351 21 8419062. e-mail address: teresa.cesario@tecnico.ulisboa.pt + Current address: Politecnico di Milano, Dipartimento di Chimica Materiali e Ingegneria Chimica "Giulio Natta". Via Mancinelli, 7 -20131 Milano MI 2 cyclohexanone and phenetole were used as extraction solvents and the relevant process variables (extraction temperature, extraction time and mass of cells/solvent volume ratio) were optimized.Polymer recovery yields of 97% and 93% were obtained with anisole and cyclohexanone, respectively, at 120-130°C using a cell/solvent ratio of 1.5% (w/v). Maximum polymer purities using these experimental conditions were 98% for both solvents. Recovery yields and polymer purity attained with chloroform (reference solvent) were 97 and 98%, respectively. Higher cell/solvent ratios of 6.0 % (w/v) showed slightly lower recovery yields and purities The average molecular weight and the thermal properties of the polymers extracted with the alternative solvents were comparable to those of the polymers obtained by chloroform extraction, showing that the applied conditions did not significantly alter the properties of the extracted P(3HB).

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Section: P(3hb) Extractionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents

Rosengart

Cesário

Almeida

et al. 2015

Biochemical Engineering Journal

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“…O acúmulo intracelular de PHA's foi verificado por análises qualitativas realizadas utilizando-se a técnica de coloração Sudan Black (SHEEHAN & STOREY, 1947). Ao final do cultivo, a suspensão celular obtida foi centrifugada e submetida à extração com clorofórmio pelo método descrito por Ramsay et al (1994), e o polímero obtido foi caracterizado por ressonância magnética nuclear (RMN) comparativamente a um padrão de P3HB.…”

Section: Condições De Cultivo E Métodos Analíticosunclassified

HIDRÓLISE ENZIMÁTICA DE PECTINA PARA A PRODUÇÃO DE POLIHIDROXIALCANOATOS POR Cupriavidus necator

Locatelli¹,

Finkler²,

Finkler³

2015

Anais Do XX Congresso Brasileiro De Engenharia Química

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RESUMO -Resíduos agroindustriais, provenientes da extração de sucos de frutas cítricas, são ricas fontes de substâncias pécticas. Objetivando o aproveitamento desses resíduos como uma alternativa de baixo custo para a produção de biopolímeros, foi estudado o uso de hidrolisados pécticos para crescimento celular e produção de polihidroxialcanoatos por Cupriavidus necator. Nesse trabalho foi empregada uma pectina cítrica comercial (Vetec ® ) e uma endo-poligalacturonase (Sigma ® ), sendo realizado um estudo das condições de hidrólise que favorecessem a obtenção de compostos redutores (CR) por meio da aplicação de planejamento fatorial e metodologia de superfície de resposta. As variáveis investigadas foram a concentração de enzima e a agitação, mantendo-se a concentração de substrato em 1% (p/v). Os resultados obtidos foram comparados com a performance enzimática realizada a uma maior concentração de substrato (10% p/v) empregando-se diferentes concentrações de enzima. Ambos os resultados indicaram que a concentração enzimática de 10 UI/g de pectina favoreceu a obtenção de CR, permitindo atingir em média 70 g/L de CR a partir de 100 g/L de pectina. O meio de cultura formulado a partir desse hidrolisado foi satisfatório para o crescimento celular de C. necator, sendo obtida uma concentração de células acima de 10 g/L e o acúmulo intracelular de polihidroxialcanoatos.

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“…The PHB production by A. latus on different industrial wastes [40] under aerobic conditions was studied. A. latus was grown at 37°C for 72 h. PHB produced were extracted as described in the method of Ramsay et al [41,42].…”

Section: Phb Production and Extractionmentioning

confidence: 99%

Optimization of Poly ?-Hydroxy Butyrate Production by Alcaligenes Latus MTCC2311 using Central Composite Design

Veeramanikandan¹,

Mrudula²,

Yuvaraj³

et al. 2013

IJCA

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Considering the industrial interest of poly-b-hydroxy butyrate (PHB) and its high production cost, work has been undertaken for the production of PHB by Alcaligenes latus (2311). Different industrial wastes (sesame, molasses, sago and paper waste) were used as a cheap substrate to minimize the production of cost and nitrogen limited minimal agar synthetic medium was also used for comparison. Accumulation of PHB granules in the organism was analyzed by sudan black method. The PHB production in various industrial waste based medium and nitrogen limited minimal agar synthetic medium was studied by crotonic acid method. The pure form of PHB was collected and qualitatively analyzed by infrared and nuclear magnetic resonance methods. Highest PHB production was found in nitrogen limited minimal agar synthetic medium. Among the various industrial wastes based media, highest yield was obtained with sesame oil waste as carbon source KeywordsPoly-β-hydroxy butyrate, PHB, Alcaligenes latus MTCC 2311, CCD, bacterial polyesters.

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Extraction of poly-3-hydroxybutyrate using chlorinated solvents

Cited by 184 publications

References 9 publications

Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents

Efficient P(3HB) extraction from Burkholderia sacchari cells using non-chlorinated solvents

HIDRÓLISE ENZIMÁTICA DE PECTINA PARA A PRODUÇÃO DE POLIHIDROXIALCANOATOS POR Cupriavidus necator

Optimization of Poly ?-Hydroxy Butyrate Production by Alcaligenes Latus MTCC2311 using Central Composite Design

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