Here, we demonstrate
a facile bottom-up strategy to fabricate Pt
nanoclusters (Pt NCs) grafted onto three-dimensional graphene foam
(3D GF) assisted by cetyltrimethyl ammonium bromide (CTAB) using the
electrodeposition method. The homogeneous grafting of Pt NC onto 3D
GF is due to the formation of hemimicelles above some CTAB concentration.
With the unique nanocluster structure and the high content of Pt0, the Pt NC/3D GF nanohybrid exhibits extremely high activity
and shows higher reusability and stability. Apart from the intrinsic
oxidase-like activity with 3,3′,5,5′-tetramethylbenzidine
(TMB) as the substrate, the Pt NC/3D GF nanohybrid can act simultaneously
as an effective polyphenol oxidase (PPO) mimic, such as tyrosinase,
catechol oxidase, and laccase. More importantly, utilizing intrinsic
catechol oxidase-like activity and the oxidase-like activity with
TMB as the substrate of the nanohybrid, distinguishing colorimetric
determination of dihydroxybenzene isomers (catechol and hydroquinone)
is performed. Distinguishing colorimetric analysis of dihydroxybenzene
isomers was first developed using nanozymes. The present work provides
a simple bottom-up approach for the reasonable fabrication of various
nanostructured nanozymes with excellent performance using the electrodeposition
method assisted with surfactants.