Facile complexation reactions for the selective spectrofluorimetric determination of albendazole in oral dosage forms and spiked human plasma

Abstract

Two simple, sensitive, and rapid spectrofluorimetric methods were developed and validated for the determination of albendazole. The first method (method I) was based on the quenching effect of albendazole on the native fluorescence of erythrosine B. The fluorescence intensity was measured at 554 nm after extraction at 527 nm. In the second method (method II) the drug was reacted with lanthanum(III) ions to form a metal complex, which was measured at 340 nm after excitation at 295 nm.The suitable pH was 3.4 (Teorell-Stenhagen buffer) and pH 5.5 (phosphate buffer solution), for method I and II, respectively. The influence of experimental factors on the fluorescence intensity of the reaction products was investigated and optimized. The linear concentration ranges were 0.2-3.5 and 0.06-0.90 mg mL À1, with detection limits of 0.049 and 0.019 mg mL À1 for method I and II, respectively. ICH guidelines were followed for validation of the developed procedures, and the results were acceptable.The Gibb's free energy change of the reactions was À24.6 and À27.5 kJ mol À1 for method I and II, respectively. These negative values indicated the high feasibility of these reactions at ambient temperature. The proposed procedures were applied successfully for the determination of albendazole in commercial dosage forms and spiked human plasma. The results showed high precision, accuracy and recovery of the reported methods without any significant interference from pharmaceutical excipients or plasma components.

Factorial design-assisted spectroscopic determination of oxybutynin hydrochloride

“…EY is a carboxylic dye that is used as an ion-pairing reagent for the spectroscopic determination of cationic drugs in an acidic medium by forming ion-pair complexes [11][12][13][14][15]. In the spectrophotometric method, the ABS of the developed complex was measured at 550 nm (figure 2).…”

“…Additionally, the experimental conditions were optimized using the design of experiments (DOEs). Some amino compounds can be determined using an acidic dye called EY as an ion-pairing agent (figure 1b) via binary complex formation [11][12][13][14][15]. DOE is a powerful data collection and analysis tool that can be used under various experimental conditions [16].…”

A simple single jar “on–off fluorescence” designed system for the determination of mitoxantrone using an eosin Y dye in raw powder, vial, and human biofluids

“…The continuous variation plotting (Job's method) 23,31 was mandated in the current designed system to predict the stoichiometry of the MTX-EY reaction under optimal operating conditions. Both the cited drug and eosin Y were introduced as parent solutions at equimolar concentrations (2.8 Â 10 À4 M).…”

“…Then again, xanthene-based dyes, for example, erythrosine B and eosin Y, were chemically used to investigate many drugs such as anthelmintic, 22,23 and antispasmodic drugs. 24 Eosin Y, an acidic dye, is the disodium salt of 2,4,5,7-tetrabromo-uoresceine (Fig.…”

“…Recently, xanthene-based dyes were used for the spectrophotometric and/or spectrouorimetric determination of proteins 25,26 and some pharmaceutical compounds. [22][23][24]27 These methods were based on developing an ion-pair complex between the dye and the compounds with a basic nature. The complex formation was coupled with a quenching effect on the native uorescence of eosin Y in a linear relationship with the coupled compound.…”