Factors regulating the steroid 11-hydroxylation by non-germinating spores ofCunninghamella elegans (LENDNER)

Abstract

In the presence of malate or citrate sporangiospores of C. elegans were able to hydroxylate cortexolone with a rate twofold exceeding that of the control, water suspended spores. Analysis of the intracellular nicotinamide coenzyme pools revealed an increased NADPH:(NADP+ + NADPH) ratio, indicating more effective NADPH-generating systems in malate- or citrate-stimulating spores. Swollen spores remaining in the pregermination state, retained higher cortexolone-hydroxylating activity in the absence of malate and citrate. In these spores degradation of endogenous alanine and glutamic acid was observed. Possible NADPH-generating systems in C. elegans sporangiospores were discussed.

The uptake of Aspergillus ochraceus spores on diatomaceous particles and their use in the 11?-hydroxylation of progesterone

“…It has been demonstrated that spores are capable of carrying out steroid hydroxylations (Jaworski et al, 1982), including those of A. ochraceus for the lla-hydroxylation of progesterone (Schleg and Knight, 1962;Vezina et al, 1963) and that the use of spores is suitable for large-scale steroid hydroxylation (Singh et al, 1968).…”