Ferulic acid inhibits bovine endometrial epithelial cells against LPS-induced inflammation via suppressing NK-κB and MAPK pathway

Yin, Peng; Zhang, Zhicong; Li, Jian Dong; Shi, Yaran; Jin, Na; Zou, Wenshu; Gao, Qian; Wen, Wang; Li, Fenghua

doi:10.1016/j.rvsc.2019.08.018

Cited by 58 publications

(38 citation statements)

References 28 publications

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“…Ligusticum wallichii is usually recognized as a drug that protects host cells against inflammation since the major effective components including ferulic acid, ligustrazine, and tetramethylpyrazine extracted from this herb are able to alleviate the inflammation-induced injury in different cells or tissues [13,[24][25][26]. However, whether L. wallichii may have a protective role in chondrocytes upon inflammatory condition remains sealed.…”

Section: Discussionmentioning

confidence: 99%

A metabolic exploration of the protective effect of Ligusticum wallichii on IL-1β-injured mouse chondrocytes

Wei¹,

Dong²,

Guan³

et al. 2020

Chin Med

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Background: Osteoarthritis (OA) is a metabolic disorder and able to be relieved by traditional Chinese medicines. However, the effect of Ligusticum wallichii on OA is unknown. Methods: Cytokine IL-1β and L. wallichii extracts were used to stimulate the primary mouse chondrocytes. MTT assay was used to measure the cell viability. The mRNA and protein level of each gene were test by qRT-PCR and western blotting, respectively. The rate of apoptotic cell was measured by flow cytometry. GC/MS-based metabolomics was utilized to characterize the variation of metabolome. Results: Here, we found that L. wallichii attenuated the IL-1β-induced apoptosis, inflammatory response, and extracellular matrix (ECM) degradation in mouse chondrocytes. Then we used GC/MS-based metabolomics to characterize the variation of metabolomes. The established metabolic profile of mouse chondrocytes showed that the abundance of most metabolites (n = 40) altered by IL-1β stimulation could be repressed by L. wallichii treatment. Multivariate data analysis identified that cholesterol, linoleic acid, hexadecandioic acid, proline, l-valine, l-leucine, pyruvate, palmitic acid, and proline are the most key biomarkers for understanding the metabolic role of L. wallichii in IL-1β-treated chondrocytes. Further pathway analysis using these metabolites enriched fourteen metabolic pathways, which were dramatically changed in IL-1β-treated chondrocytes and capable of being reprogrammed by L. wallichii incubation. These enriched pathways were involved in carbon metabolisms, fatty acid biosynthesis, and amino acid metabolisms. Conclusions: These findings provide potential clues that metabolic strategies are linked to protective mechanisms of L. wallichii treatment in IL-1β-stimulated chondrocytes and emphasize the importance of metabolic strategies against inflammatory responses in OA development.

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Section: Discussionmentioning

confidence: 99%

A metabolic exploration of the protective effect of Ligusticum wallichii on IL-1β-injured mouse chondrocytes

Wei¹,

Dong²,

Guan³

et al. 2020

Chin Med

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“…It can also activate epithelial cells to release a large number of cytokines and inflammatory mediators which exacerbate the inflammatory response and inflammatory damage to local tissues. [3][4][5] LPS is the best characterised PAMP which is recognised by the specific PRR TLR4. 6 TLR4 plays a crucial role in innate immunity and inflammation, triggering the activation of NF-jB and activator protein-1 signal transduction pathways.…”

Section: Introductionmentioning

confidence: 99%

Effect of berberine on LPS-induced expression of NF-κB/MAPK signalling pathway and related inflammatory cytokines in porcine intestinal epithelial cells

Zhu

et al. 2020

Innate Immun

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Berberine is an alkaloid extracted from medicinal plants such as Coptis chinensis and Phellodendron chinense. It possesses anti-inflammatory, anti-tumour and anti-oxidation properties, and regulates Glc and lipid metabolism. This study explored the mechanisms of the protective effects of berberine on barrier function and inflammatory damage in porcine intestinal epithelial cells (IPEC-J2) induced by LPS. We first evaluated the effects of berberine and LPS on cell viability. IPEC-J2 cells were treated with 5 μg/ml LPS for 1 h to establish an inflammatory model, and 75, 150 and 250 μg/ml berberine were used in further experiments. The expression of IL-1β, IL-6 and TNF-α was measured by RT-PCR. The key proteins of the NF-κB/MAPK signalling pathway (IκBα, p-IκBα, p65, p-p65, c-Jun N-terminal kinase (JNK), p-JNK, p38, p-p38, ERK1/2 and p-ERK1/2) were detected by Western blot. Upon exposure to LPS, IL-1β, IL-6 and TNF-α mRNA levels and p-IκBα p-p65 protein levels were significantly enhanced. Pre-treatment with berberine reduced the expression of inflammatory factors and was positively correlated with its concentration, and dose dependently inhibited the expression of IκBα, p-IκBα, p-p65, p-p38 and JNK. These results demonstrated that pre-treating intestinal epithelial cells with berberine was useful in preventing and treating diarrhoea induced by Escherichia coli in weaned pigs.

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“…to other cells so that they can play further roles in recipient cells [40,41]. Endometrial epithelial cells, as the first barrier to contact with pathogens, can communicate with immune cells by releasing cytokines such as TNF-α, IL-1, IL-6, and IL-8, so that they exert an immune response function [42,43]. In order to confirm the role of endometrial epithelium-derived exosomes in regulating the immune function in the uteri of cows with endometritis, we successfully isolated and identified endometrial epithelium-derived exosomes from the supernatant of the cultured endometrial epithelial cells and co-incubated them with the uterine-derived Treg cells from the healthy cows, respectively.…”

Section: Discussionmentioning

confidence: 99%

The effects of endometrial epithelium-derived exosomes on the recruitment and activation of T Lymphocytes in the uteri of dairy cows

Xiao

et al. 2020

Preprint

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As a common group-occurring disease, endometritis is caused by endometrial cell injury and chronic inflammation of uteri infected by pathogenic microorganisms, and leads to reproductive failure in dairy cows. Changes in the immune response function of the uteri affect the occurrence and development of endometritis. It was recently discovered that exosomes act as a functional regulator secreted by a variety of cells. However, its regulatory mechanism in the local immune response of the uteri is still unclear. In this study, flow cytometry was adopted to identify that the number of T lymphocytes in the uteri with endometritis increased while the compositional proportion also changed, namely that of Tc cell increased very significantly and that of Th and Treg cells decreased very significantly. Moreover, the functional marker factor Foxp3 of Treg cells was extremely significantly decreased, and the functional marker factor of Th17 cells was significantly increased. The mRNA expression of immune tolerance regulators PD1, CTLA4 and Galectin-1 in the group of Treg cells which were co-incubated with LPSstimulated endometrial epithelium-derived exosomes was very significantly lower than in the group of Treg cells which were co-incubated with normal EEC-derived exosomes. Furthermore, the protein expression of PD1, CTLA4, Galectin-1, Foxp3, and IL-17 was consistent with the results in the Treg cells co-incubated with different source exosomes in mice. Therefore, it was demonstrated that endometrial epithelium-derived exosomes act as a vital regulator of changes in the composition and function of T lymphocyte subsets in the uterus of dairy cows. Title page The effects of endometrial epithelium-derived exosomes on the recruitment and activation of T Lymphocytes in the uteri of dairy cows Running head: Exosomes activate T Lymphocytes

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Ferulic acid inhibits bovine endometrial epithelial cells against LPS-induced inflammation via suppressing NK-κB and MAPK pathway

Cited by 58 publications

References 28 publications

A metabolic exploration of the protective effect of Ligusticum wallichii on IL-1β-injured mouse chondrocytes

A metabolic exploration of the protective effect of Ligusticum wallichii on IL-1β-injured mouse chondrocytes

Effect of berberine on LPS-induced expression of NF-κB/MAPK signalling pathway and related inflammatory cytokines in porcine intestinal epithelial cells

The effects of endometrial epithelium-derived exosomes on the recruitment and activation of T Lymphocytes in the uteri of dairy cows

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