Fibronectin Immobilization using Water-soluble Carbodiimide on Poly-L-lactic                 Acid for Enhancing Initial Fibroblast Attachment

Nagai, Megumi; Hayakawa, Tohru; Makimura, Mizue; Yoshinari, Masao

doi:10.1177/0885328206055316

Cited by 34 publications

(29 citation statements)

References 31 publications

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“…NaOH treatment is a simple technique for modifying degradable polymeric materials such as PLA and PLGA. Nagai et al 21) evaluated the hydrolysis time of PLA for surface hydrolysis. They reported that 3 hours of NaOH treatment produces the lowest contact angle with respect to double-distilled water.…”

Section: Discussionmentioning

confidence: 99%

“…Fuse et al 25) and Nagai et al 21) reported an improvement in the biological activity of P LA, such as cell attachment, biocompatibility, immobilized fibronectin, and cell-adhesive protein, on the surface of a PLA film following NaOH surface hydrolysis and condensation reactions using water-soluble carbodiimide. The result demonstrated that fibronectin immobilization enhanced cell attachment and proliferation on the surface of the PLA film.…”

Section: Discussionmentioning

confidence: 99%

“…Surface hydrolysis of PLCL films was performed according to a previous method 21) . The simple scheme is shown in Fig.…”

Section: Preparation Of Plcl Filmsmentioning

confidence: 99%

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Enhancement of Apatite Precipitation on an Alkaline Hydrolyzed Poly (Lactic acid-ε-Caprolactone) Film in Simulated Body Fluid

Tsurumi

Fusé

2014

J. Hard Tissue Biology.

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Biodegradable materials, such as poly (lactic acid) (PLA), have been applied to regenerate bone. However, pure PLA is brittle, so it can be modified by blending -caprolactone (PCL). These biodegradable materials have no known functional activity. In the present study, a copolymer of PLA/PCL (PLCL) film was fabricated by dissolving the materials in chloroform. Carboxylic acid groups (COOH) of PLCL were chemically introduced onto the PLCL film surface by 0.5N NaOH hydrolysis. Apatite formation was evaluated on an alkaline hydrolyzed PLCL film (PLCL-COOH) after immersion in Hanks' balanced salt solution (HBSS, pH7.4) without organic species. Water adsorption in (PBS) PLCL-COOH phosphate buffered saline was also monitored by comparing PLCL. PLCL-COOH provided a greater degree of apatite precipitation than PLCL after 3 and 7-day immersion. Weight loss was significantly different between PLCL-COOH and PLCL films from 3 days until 124 days of immersion in PBS (p<0.05). The contact angle of the PLCL-COOH surface with respect to double distilled water significantly decreased compared with PLCL (p<0.05). Thus, it was predicted that PLCL-COOH would show a better biological response and be applicable in new types of dental materials in dental treatment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Enhancement of Apatite Precipitation on an Alkaline Hydrolyzed Poly (Lactic acid-ε-Caprolactone) Film in Simulated Body Fluid

Tsurumi

Fusé

2014

J. Hard Tissue Biology.

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“…The present results were consistent with our previous study. Fibronectin immobilization on PLA surface significantly enhanced the number of initially (90 min cell culture) attached human gingival fibroblast 18) . Other studies reported the efficacy of collagen or fibronectin immobilization for cell attachment and proliferation.…”

Section: Discussionmentioning

confidence: 98%

“…Contact angles were significantly decreased by NaOH treatment for 3 hr. Our previous study reported the introduction of carboxylic acid groups onto a PLA film surface by NaOH hydrolysis method 18) . PLA film was treated by NaOH for 3 hr.…”

Section: Discussionmentioning

confidence: 99%

MC3T3-E1 Cell Assay on Collagen or Fibronectin Immobilized Poly (Lactic Acid-ε-Caprolactone) Film

Fusé

Hayakawa

Hashizume-Takizawa

et al. 2015

J. Hard Tissue Biology.

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In the present study, collagen (Coll) or fibronectin (FN) was immobilized onto poly(lactic acid)/ -caprolactone (PLCL) copolymer surface and the attachment and proliferation of osteoblast-like cells on Coll or FN immobilized PLCL (Coll-PLCL, FN-PLCL) were evaluated. Coll or FN immobilized PLCL was prepared through a condensation reaction between the carboxylic acid groups on the hydrolyzed PLCL (PLCL-COOH) surface and the amino groups of Coll or FN using water soluble carbodiimide. The contact angle of the PLCL-COOH, Coll-PLCL and FN-PLCL surfaces with respect to double distilled water significantly decreased compared with PLCL (p<0.05). Number of attached cells onto Coll-PLCL or FN-PLCL films was significantly greater than on PLCL films from at the time of 90 min to 7days (p<0.05). The morphological differences of attached osteoblast-like cells were observed between PLCL films and Coll-PLCL or FN-PLCL films at the early stage of cell assay. The cell attached on Coll-PLCL and FN-PLCL films appeared more flat than on PLCL films. Additionally, many actin filaments and stress fibers were observed on Coll-PLCL or FN-PLCL films during 3 and 7 days of cell assay. Actin filaments and stress fibers were few on PLCL films. These data demonstrated the effectiveness of collagen and fibronectin immobilization of PLCL for cell attachment and proliferation. This indicates that collagen or fibronectin immobilization of PLCL films was effective for biological activities of osteoblast-like cells.

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Adding Functions to Biomaterial Surfaces through Protein Incorporation

et al. 2016

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The concept of biomaterials has evolved from one of inert mechanical supports with a long-term, biologically inactive role in the body into complex matrices that exhibit selective cell binding, promote proliferation and matrix production, and may ultimately become replaced by newly generated tissues in vivo. Functionalization of material surfaces with biomolecules is critical to their ability to evade immunorecognition, interact productively with surrounding tissues and extracellular matrix, and avoid bacterial colonization. Antibody molecules and their derived fragments are commonly immobilized on materials to mediate coating with specific cell types in fields such as stent endothelialization and drug delivery. The incorporation of growth factors into biomaterials has found application in promoting and accelerating bone formation in osteogenerative and related applications. Peptides and extracellular matrix proteins can impart biomolecule- and cell-specificities to materials while antimicrobial peptides have found roles in preventing biofilm formation on devices and implants. In this progress report, we detail developments in the use of diverse proteins and peptides to modify the surfaces of hard biomaterials in vivo and in vitro. Chemical approaches to immobilizing active biomolecules are presented, as well as platform technologies for isolation or generation of natural or synthetic molecules suitable for biomaterial functionalization.

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Fibronectin Immobilization using Water-soluble Carbodiimide on Poly-L-lactic Acid for Enhancing Initial Fibroblast Attachment

Cited by 34 publications

References 31 publications

Enhancement of Apatite Precipitation on an Alkaline Hydrolyzed Poly (Lactic acid-ε-Caprolactone) Film in Simulated Body Fluid

Enhancement of Apatite Precipitation on an Alkaline Hydrolyzed Poly (Lactic acid-ε-Caprolactone) Film in Simulated Body Fluid

MC3T3-E1 Cell Assay on Collagen or Fibronectin Immobilized Poly (Lactic Acid-ε-Caprolactone) Film

Adding Functions to Biomaterial Surfaces through Protein Incorporation

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