Field survey of ranunculus mild mosaic virus, tomato spotted wilt virus and cucumber mosaic virus infections in Ranunculus asiaticus L. in Japan by newly developed multiplex RT-PCR

Hayahi, Saki; Matsushita, Yosuke; Kanno, Yoshihiro; Kushima, Yoshiyuki; Teramoto, Shinji; Takeshita, M

doi:10.1007/s10658-017-1268-8

Cited by 8 publications

(7 citation statements)

References 18 publications

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“…Previous studies have reported the RanMMV infection of ranunculus in Italy (Turina et al 2006;Minutolo et al 2016), China (Wang et al 2008) and Japan (Kawano and Kanno 2014;Hayahi et al 2018). Our study is the first to detect RanMMV in R. japonicus, R. tachiroei, R. cantoniensis, G. carolinianum, V. sativa, V. tetrasperma and V. hirsute, indicating that, in addition to Ranunculaceae plants, RanMMV infects Geraniaceae and Fabaceae plants.…”

Section: Resultssupporting

confidence: 60%

“…Ranunculus has been shown to be infected by several viruses (Vaira et al 1997;Turina et al 2006;Ciuffo et al 2011;Hayahi et al 2017Hayahi et al , 2018, including the ranunculus mild mosaic virus (RanMMV; Kawano and Kanno 2014), cucumber mosaic virus, broad bean wilt virus (Ushiyama et al 1989), ranunculus leaf distortion virus (Hayahi et al 2017) and tomato spotted wilt virus (TSWV) (Hayahi et al 2018) in Japan. Recently, in Miyazaki Prefecture, Kawano and Kanno (2014) and Hayahi et al (2018) reported that RanMMV is extensively found in ranunculus fields. RanMMV is a linear, single-stranded (+) RNA virus belonging to the genus Potyvirus, which is currently known to be transmitted via aphids and causes mosaics in plant leaves upon infection (Turina et al 2006).…”

Section: Introductionmentioning

confidence: 99%

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Detection of ranunculus mild mosaic virus in weed species in Japan

Kamikawa

Ohta

Kurogi

et al. 2021

Letters Applied Microbiology

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Significance and Impact of the Study: Ranunculus (Ranunculus asiaticus L.) is one of the most popular cut flowers in Japan. In this study, ranunculus mild mosaic virus (RanMMV) in Ranunculaceae weed plants was found in ranunculus cultivation fields and noncultivation regions in Japan. Since Ranunculaceae weeds grow all season, but ranunculus plants are only seasonal, it is thought that these weeds could be a source of RanMMV infection in ranunculus plants. Pairwise comparison of CP genes from ranunculus isolates and weeds showed high nucleotide and amino acid identities, suggesting that RanMMV could be transmitted between Ranunculaceae weeds and R. asiaticus plants.

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Section: Resultssupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

Detection of ranunculus mild mosaic virus in weed species in Japan

Kamikawa

Ohta

Kurogi

et al. 2021

Letters Applied Microbiology

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“…Hayahi et al . () found that the end‐point dilution limits for performance of single and multiplex RT‐PCR were 10 −4 and 10 −2 , respectively; nevertheless, despite the decrease in sensitivity for mRT‐PCR, this method still gives a good performance compared to ELISA.…”

Section: Discussionmentioning

confidence: 99%

“…Hayahi et al . () also proposed an mRT‐PCR for the detection of three viruses infecting ranunculus (RanMMV, TSWV and CMV). However, in the current study, CMV was tested by specific RT‐PCR but not considered for mRT‐PCR as it was not detected in samples.…”

Section: Discussionmentioning

confidence: 99%

RT‐PCR tests for sensitive detection of the major Ranunculus‐infecting viruses: field and in vitro applications

Sacco

Borghi

Rabaglio³

et al. 2018

Plant Pathology

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Buttercup (Ranunculus asiaticus, family Ranunculaceae) hybrids are considered a popular and valuable ornamental crop in Europe and are cultivated both for cut flowers and for pot or border plants. In the Liguria region of northern Italy, Ranunculus hybrid cultivation has dramatically increased over recent years and the related sanitary issues regarding virus infection need to be carefully monitored in order to maintain commercial competitiveness. Presently, ELISA is the most used diagnostic tool for virus diagnosis, but if ‘mother plants’ with high economic value are to be tested, more sensitive molecular diagnostic tools are needed, together with efficient protocols for in vitro culture to eradicate viruses. In this study, new RT‐PCR protocols were tested on virus‐infected Ranunculus hybrid plants, indexed for the 13 most important Ranunculus‐infecting viruses. Rapid, uniform and effective procedures for molecular diagnosis were devised in order to promote an easy technology transfer from scientific institutions to laboratories of small and medium private enterprises. Moreover, an in vitro culture procedure was established for this species, which proved to be extremely effective in virus eradication. Advanced technology and sustainable quality production of high economic value plants are keys for future competitiveness in floriculture.

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“…To test whether RanMMV-like virus could be detected from the above extracted RNAs, Reverse transcription polymerase chain reaction (RT-PCR) was performed with RanMMV-speci c primer sets RanMMV-F/RanMMV-R [12], using QIAGEN OneStep RT-PCR kit (QIAGEN). We performed direct-sequence analysis of these PCR products.…”

Section: Introductionmentioning

confidence: 99%

First Report of the Complete Genome Sequences of Anemone Mosaic Virus and Ranunculus Mild Mosaic Virus Isolated From Anemone Imported From the Netherlands

Imamura¹,

Oishi²,

Fujiwara³

et al. 2021

Preprint

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The anemone mosaic virus (AnMV) and ranunculus mild mosaic virus (RanMMV) infects the anemone plant with characteristic mosaic patterns on leaves. Two complete genome sequences of the two viruses imported from the Netherlands, were determined based on deep sequencing for the first time. Each of AnMV and RanMMV had 9,698 and 9,537 nucleotides (nt), excluding the poly(A) tail. They shared 80.0% nt/amino acid (aa) sequence identities or more, which are above the species demarcation value, with only AnMV and RanMMV reported previously in coat protein region, but having 68.0% nt/aa sequence identities or less with other potyviruses in each coding region of the complete sequences. Additionally, phylogenetic analysis showed that AnMV and RanMMV were included in other known potyviruses. These results suggest that both of AnMV and RanMMV were independent species belonging to the genus Potyvirus.

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Field survey of ranunculus mild mosaic virus, tomato spotted wilt virus and cucumber mosaic virus infections in Ranunculus asiaticus L. in Japan by newly developed multiplex RT-PCR

Cited by 8 publications

References 18 publications

Detection of ranunculus mild mosaic virus in weed species in Japan

Detection of ranunculus mild mosaic virus in weed species in Japan

RT‐PCR tests for sensitive detection of the major Ranunculus‐infecting viruses: field and in vitro applications

First Report of the Complete Genome Sequences of Anemone Mosaic Virus and Ranunculus Mild Mosaic Virus Isolated From Anemone Imported From the Netherlands

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