FilmArray® respiratory panel performance in respiratory samples from neonatal care units

Abstract

FilmArray Respiratory Panel (RP) (Idaho Technology, Inc., Salt Lake City, UT, USA) performance was retrospectively evaluated in respiratory samples collected from neonates in 2 reference neonatology units. Using the FilmArray RP assay, 121/152 (79.6%) samples were positive for at least 1 respiratory virus, while 31/152 (20.4%) were negative. FilmArray RP results were concordant in 68/72 (94.4%) respiratory samples tested with laboratory-developed real-time PCR assays, while in 4/72 (5.6%) samples, the FilmArray RP assay detected an additional virus (2 human rhinovirus/enterovirus and 2 bocavirus). In addition, FilmArray RP results for 70 of 80 (87.5%) respiratory samples tested were concordant with the Seegene Seeplex RV15® detection assay (Seegene, Inc., Seoul, South Korea), while 10/80 (12.5%) were discordant. The advantages of the FilmArray RP are the rapid detection of respiratory viruses (1 hour), the wide number of pathogens detectable in a single assay, and the reduced hands-on time.

Enterovirus-D68 (EV-D68) in pediatric patients with respiratory infection: The circulation of a new B3 clade in Italy

“…Respiratory samples were extracted using a QIAsymphony ® instrument with a QIAsymphony ® DSP Virus/Pathogen Midi Kit (Complex 400 protocol) according to the manufacturer's instructions (QIAGEN, Qiagen, Hilden, Germany). Panels of laboratory-developed real-time RT-PCR or real-time PCR [22,23] were used to detect and quantify the following viruses: influenza virus A and B (including subtype determination), human Rhinovirus (hRV), human parainfluenza virus (hPIV) 3, respiratory syncytial virus (RSV) types A and B, human coronavirus (hCoV)-OC43, -229E, -NL63, and -HKU1, human metapneumovirus (hMPV) and human adenoviruses (hAdVs). Extracted samples were also amplified using EV-D68-specific real-time RT-PCR as previously described [24].…”

Frequency of respiratory viruses among patients admitted to 26 Intensive Care Units in seven consecutive winter-spring seasons (2009–2016) in Northern Italy

“…Respiratory samples were tested with a panel of laboratory-developed real-time RT-PCR or real-time PCR able to detect and quantify the following viruses [9,10]: influenza virus A and B, including subtype determination, hPIV-3, hRSV types A and B, hCoV types −OC43, −229E, −NL63, and −HKU1, hMPV, HRV and CMV (in a subset of samples).…”

Persistent rhinovirus infection in pediatric hematopoietic stem cell transplant recipients with impaired cellular immunity

“…In the latter case, follow-up samples were collected at subsequent outpatient clinical evaluations. Respiratory samples were routinely tested using a panel of RT-PCR and real-rime RT-PCR assays [13,19] for human influenza virus types A and B, human metapneumovirus A and B, human respiratory syncytial virus A and B, four human coronaviruses (OC43, 229E, NL63, HKU1), human parainfluenza types 3, human adenovirus, rhinoviruses and enteroviruses.…”