First detection and genetic characterization of porcine parvovirus 7 from Korean domestic pig farms

Ouh, In-Ohk; Park, Sung Yong; Lee, Ju‐Yeon; Song, Jae Young; Cho, In‐Soo; Kim, Hye‐Ryung; Park, Choi‐Kyu

doi:10.4142/jvs.2018.19.6.855

Cited by 30 publications

(38 citation statements)

References 8 publications

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“…To investigate the genetic diversity of porcine parvoviruses, this study reconstructed the genetic relationships of seven PPV genotypes with eight assigned genera of subfamily Parvovirinae. The clustering patterns of porcine parvoviruses in this study was in line with the previous publications of which PPV1-PPV7 belonged to different genera [13,[19][20][21]. The genetic heterogeneity of porcine parvoviruses was further revealed by the fact that the virus was not only evolving at high substitution rates (10 − 3 to 10 − 5 substitutions/site/year, Fig.…”

Section: Discussionsupporting

confidence: 91%

“…Of the recently discovered PPV7, in combination with the first report of PPV7 in South Korea [13], this result confirmed again the presence of PPV7 in different provinces. In a previous study, PPV7 was found at a significantly higher rate from finishing pigs (74.9%) rather than aborted fetuses (24.0%) [13]. Additionally, it was reported that most PPV DNA-positive sera were identified in adult pigs aged 9-18 weeks [14].…”

Section: Discussionsupporting

confidence: 71%

“…Additionally, it was reported that most PPV DNA-positive sera were identified in adult pigs aged 9-18 weeks [14]. Accordingly, the type of sample and age group of the pigs could affect to the detection rates for PPVs, which might be the main reason this study detected PPV7 at a very low rate of 1.32% compared to the previous study [13]. Genotypes 1-7 of PPV were detected in many countries, such as USA [16], Poland [14] and China [17], etc.…”

Section: Discussionmentioning

confidence: 61%

“…Genotypes 1-7 of PPV were detected in many countries, such as USA [16], Poland [14] and China [17], etc. However, to our best knowledge, only PPV1 [9,12], PPV2 [18] and PPV7 [13] have been reported in South Korea to date. As a result, further study is required to elucidate the prevalence of the other PPV genotypes in this country.…”

Section: Discussionmentioning

confidence: 85%

“…It has been suggested that PPVs are divided into 7 genotypes, of which genotype 7 was most recently identified. In South Korea, after the publications in 2001 [12] and 2017 [9,13], few studies were focused on the detection of PPVs. This study attempted to investigate the presence of PPVs by molecular-based methods and investigate several genetic properties of PPVs.…”

Section: Introductionmentioning

confidence: 99%

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PCR-based detection and genetic characterization of porcine parvoviruses in South Korea in 2018

et al. 2020

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Background: with the advantage of sequencing technology, many novel porcine parvoviruses (PPV) rather than PPV1 has been reported. This study ultilized specific PCR-based method and gene-based analysis to study the presence and genetic diversity of porcine parvoviruses in South Korea in 2018. Results: The present study was conducted in 2018 and found PPV1 and PPV7 in nine out of 151 field samples (organs and semen) by the PCR method. Among these, the complete genome sequences of five strains (N2, N91, N108, N133, and N141) were recovered. Phylogenic analysis revealed that the strains N2, N91, and N108 belong to the PPV1 genotype, while N133 and N141 belong to PPV7 genotype. The PPV7 strains collected in this study had deletion mutations in the VP2 gene but differed from that of PPV7 strains collected in 2017. Among the PPV1 strains, the amino acid variations in the B cell epitopes of the VP2 protein were observed between three Korean PPV1 field strains (N2, N91, and N108) and the reference PPV1 strains. Those substitutions resulted in six out of 12 predicted epitopes having significant differences in antigenic index compared to the other PPV1 strains. Conclusions: This study confirmed the presence of different genotypes of porcine parvoviruses in South Korea. The PPVs circulating in South Korea were phylogenetically classified as PPV1 and PPV7 genotypes. Three Korean PPV1 strains collected in 2018 were predicted to have antigenic alteration in VP2 compared to several reference strains of PPV1.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 71%

Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

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PCR-based detection and genetic characterization of porcine parvoviruses in South Korea in 2018

et al. 2020

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First investigation of the prevalence of parvoviruses in slaughterhouse pigs and genomic characterization of ungulate copiparvovirus 2 in Vietnam

et al. 2021

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Ungulate protoparvovirus 1, also known as porcine parvovirus 1 (PPV1), is considered to be 18 one of the major causes of reproductive failure in pig breeding herds. In addition, in pigs, other 19 parvoviruses have also been identified, including Ungulate tetraparvovirus 3 or PPV2, 20 Ungulate tetraparvovirus 2 or PPV3, and Ungulate copiparvovirus 2 or PPV4 but their 21 significance for pigs is unknown. In the present study, the prevalence of PPV1-4 was 22 investigated using a total of 231 lung and serum samples collected from slaughter houses in 13 23 provinces throughout Vietnam. The overall prevalence was 54.5% (126/231) for PPV1, 28.0% 24 (65/231) for PPV2, 17.7% (41/231) for PPV3, and 7.8% (18/231) for PPV4. While PPV1 and 25 PPV2 appeared in 11/13 provinces, PPV4 was detected in only 3/13 provinces. PPV1, PPV2 26 and PPV3 co-circulation was frequently observed with PPV1/PPV2 co-infection, 27 predominating with 20.8% (48/231). All four PPVs were only detected together in one sample 28 from Thua Thien Hue. Three nearly complete PPV4 genomes of 5,453 bp in size were obtained 29 and deposited in GenBank. Genomic alignment and comparison of the three sequences showed 30 high identities at both the nucleotide (99.5-99.6%) and the deducted amino acid levels (99.6-31 99.9%) of open reading frames 1-3 and also with Vietnamese or Chinese strains (98.9-99.3% 32 and 99.4-99.7%, respectively). Phylogenetic analysis further confirmed a close relationship 33 between Vietnamese and Chinese PPV4 strains. These results are the first to report the 34 prevalence of PPV1, PPV2, PPV3, and PPV4 and nearly complete genomic sequences of PPV4 35 in pigs from slaughterhouses in Vietnam.

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Genetic analysis of porcine parvoviruses detected in South Korean wild boars

Park

Song

et al. 2021

Arch Virol

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Porcine parvovirus 1 (PPV1) is a major cause of reproductive failure in pigs; to date, six novel porcine parvoviruses (PPV2-PPV7) have been identi ed. Here, we detected 11 PPV1, ve PPV3, three PPV4, six PPV5, ve PPV6, and one PPV7 strain in Korean wild boars. PPV1, -3, and -5, and PPV6, from Korean wild boars harbor conserved motifs within the Ca 2+ binding loop and the catalytic center of the PLA1 motif. Intrarecombination among PPV7 strains was also identi ed. Genetic characterization revealed that PPV1 from Korean wild boars may be similar to virulent PPV strains.

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First detection and genetic characterization of porcine parvovirus 7 from Korean domestic pig farms

Cited by 30 publications

References 8 publications

PCR-based detection and genetic characterization of porcine parvoviruses in South Korea in 2018

PCR-based detection and genetic characterization of porcine parvoviruses in South Korea in 2018

First investigation of the prevalence of parvoviruses in slaughterhouse pigs and genomic characterization of ungulate copiparvovirus 2 in Vietnam

Genetic analysis of porcine parvoviruses detected in South Korean wild boars

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