Flavobacterium aquariorum sp. nov., isolated from freshwater of the North Han River

Joung, Yochan; Jang, Hye-Jin; Song, Jaeho; Cho, Jang-Cheon

doi:10.1007/s12275-019-8436-9

Cited by 12 publications

(3 citation statements)

References 55 publications

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“…Degradation of DNA was tested on DNase test agar (HiMedia). Hydrolysis of casein (3.0 % skimmed milk, w/v), Tween 20 (1.0 %, w/v), Tween 80 (1.0 %, w/v), gelatin (1 % w/v) and starch (1.0 %, w/v) was tested using R2A agar as the basal medium and evaluated after 5 days incubation at 25 °C [31,34].…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…Other phenotypic tests were performed using API 20NE and API ZYM strips (bioMérieux) and GENIII MicroPlates (Biolog) according to the manufacturers' instructions with modifications to the optimum incubation temperature and time for the strains (at 25 °C for up to 7 days) [31,34]. For comparative phenotypic characterization, F. crassostreae LPB0076 T [35], the closest type strain based on 16S rRNA gene sequence analysis of the new isolates, was grown and analysed in parallel with strains F-60 T and F-392 under identical conditions.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

“…Hydrolysis of casein (3.0 % skimmed milk, w/v), Tween 20 (1.0 %, w/v), Tween 80 (1.0 %, w/v), gelatin (1 % w/v) and starch (1.0 %, w/v) was tested using R2A agar as the basal medium and evaluated after 5 days incubation at 25 °C [31, 34]. Other phenotypic tests were performed using API 20NE and API ZYM strips (bioMérieux) and GENIII MicroPlates (Biolog) according to the manufacturers’ instructions with modifications to the optimum incubation temperature and time for the strains (at 25 °C for up to 7 days) [31, 34]. For comparative phenotypic characterization, F.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

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Flavobacterium muglaense sp. nov. isolated from internal organs of apparently healthy rainbow trout

Duman

Altun

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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Two yellow-pigmented isolates, F-60T and F-392, were isolated from the internal organs of an apparently healthy rainbow trout (Oncorhynchus mykiss). The strains were identified as members of the genus Flavobacterium based on the results of 16S rRNA gene sequence analysis. Strains F-60T and F-392 had the highest 16S rRNA gene sequence identity level of 97.4 % to the type strain of Flavobacterium crassostreae LPB0076T. A polyphasic taxonomic approach including phenotypic, chemotaxonomic and genomic characterization was employed to ascertain the taxonomic position of the strains within the genus Flavobacterium . Digital DNA–DNA hybridization (dDDH) and average nucleotide identity based on blast (ANIb) values for strains F-60T and F-392 were calculated as 100 %. However, dDDH and ANI analyses between the strains and their close neighbours confirmed that both strains represent a novel species in the genus Flavobacterium . The strains shared the highest dDDH and ANIb levels of 23.3 and 77.9%, respectively, with the type strain of Flavobacterium frigidarium DSM 17623T while those values for F. crassostreae LPB0076T were obtained as 21.4–21.5 % and 76.3 %. The DNA G+C content of the strains was 34.5 mol%. Chemotaxonomic and phylogenomic analyses of these isolates confirmed that both strains are representatives of a novel species for which the name Flavobacterium muglaense sp. nov. is proposed, with F-60T as the type strain (=JCM 34196T=KCTC 82256T).

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Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Section: Physiology and Chemotaxonomymentioning

confidence: 99%