Flavonoids analysis and antioxidant, antimicrobial, and anti-inflammatory activities of crude and purified extracts from Veronicastrum latifolium

Yin, Lujun; Han, Huiying; Zheng, Xue; Wang, Gen; Li, Ying; Wang, Wei

doi:10.1016/j.indcrop.2019.04.007

Cited by 32 publications

(21 citation statements)

References 35 publications

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“…Flavonoid has been reported to exhibit a wide range of biological activities, including anti-bacterial, antiviral, anti-inflammatory, anti-allergic, anticancer and vasodilatory actions (Ayna et al, 2020;Abotaleb et al, 2019;Yin et al, 2019). The flavonoids that include acacetin, apigenin, chrysin, kaempherol, pinocembrin, hesperidin and quercetin contribute to its antioxidant activity (Kocot et al, 2018;Pasupuleti et al, 2017;Viuda-Martos et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activities of Bingöl Royal Jelly on SH-SY5Y Cells

Çalışkan

Ayna

Tunç

et al. 2021

International Journal of Nature and Life Sciences

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Royal Jelly is a bee product with high protein content is a unique nutrient for the queen honeybee. It leads to a substantial elongation of the lifetime of the queen in comparison to the worker honeybees via anti-inflammatory, anti-oxidant, anti-cancer and anti-microbial properties. Flavonoids naturally have reported to have anticancer activities thanks to their potent antioxidant activity. The antioxidant activity of RJ is attributed to its rich flavonoid content however anti-oxidant activities of the Bingöl RJ has yet to be explored in detail. The goal of the current study was to investigate the antioxidant activities of Bingöl RJ on SH-SY5Y neuroblastoma cells. The results of our study revealed that Bingöl RJ at a concentration of 0.3 mg/ml significantly augmented ROS level in SH-SY5Y cells while 0.5 mg/ml of RJ had almost no effect on ROS levels. The levels of malondialdehyde in SH-SY5Y cells considerably increased in the presence of 0.3 mg/ml RJ while 0.5 mg/ml RJ had no significant impact on MDA levels in SH-SY5Y cells. The results showed that RJ treatment 0.3 mg/ml) significantly lowered the activities of SOD and CAT activity while 0.5 mg/ml of RJ had negligible effect indicating that RJ could protect the cell membranes from radical mediated cell injuries.

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Section: Introductionmentioning

confidence: 99%

Antioxidant Activities of Bingöl Royal Jelly on SH-SY5Y Cells

Çalışkan

Ayna

Tunç

et al. 2021

International Journal of Nature and Life Sciences

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“…The formation process of m / z 119.04924 and 121.02824 were shown in Figure 6 . The fragment ions of compound 2 were compared with the reported data, the diagnostic ion at m / z 286.04630, thus, compound 2 was identified as hispidulin [ 40 ].…”

Section: Resultsmentioning

confidence: 99%

Extraction, Radical Scavenging Activities, and Chemical Composition Identification of Flavonoids from Sunflower (Helianthus annuus L.) Receptacles

et al. 2021

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This study was focused on extraction, radical scavenging activities, and chemical composition identification of total flavonoids in sunflower (Helianthus annuus L.) receptacles (TFSR). We investigated the optimal extract parameters of TFSR using response surface methodology. The highest yield of TFSR was 1.04% with the ethanol concentration 58%, the material-to-liquid ratio 1:20 (v/w), the extraction time 2.6 h, and the extraction temperature 67 °C. The results of radical scavenging activities showed that ethyl acetate fraction (EAF) was the strongest by using 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2’-azino-bis (3-ethylbenzo thiazoline-6-sulfonic acid) (ABTS) and iron ion reducing analysis. The EAF had the highest flavonoids contents. Four fractions A, B, C and D were enrichment from EAF by polyamide resin. Fraction B had the highest flavonoids content. Thirteen chemical components of flavonoids in fraction B were first identified by Ultimate 3000 Nano LC System coupled to a Q Exactive HF benchtop Orbitrap mass spectrometer (UHPLC-HRMS/MS). Among of the thirteen chemical components, isoquercetin and daidzein were identified accurately by comparing with standard samples. Radical scavenging analysis showed that isoquercetin and EAF had strong activities. Therefore, sunflower receptacles can be used as a source of natural flavonoids. TFSR as a natural radical scavenger has potential applications in pharmaceutical industry.

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“…The signaling molecule NO mediates and regulates in ammatory responses [14], while the proin ammatory cytokines TNF-α, IL-1β and IL-6 can lead to tissue damage when their levels become excessive or remain chronically high. Using bacterial LPS to stimulate the production of NO and these cytokines [15], we found that all three Dai antidotes inhibited their production.…”

Section: Nomentioning

confidence: 99%

Study On Detoxification Mechanism of Three Traditional Dai Antidotes

Zhang¹,

Zhao²,

Zhu

et al. 2021

Preprint

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Background: Dai antidotes are the most distinctive medicine and treatment in traditional Dai medicine. Clerodendrum chinense var. simplex, Marsdenia tenacissima (Roxb.) Moon and Arundina graminifolia (D. Don) Hochr are three Dai antidotes widely used for their “detoxifying effects”, and their use is rooted in a theoretical system significantly different and much less understood than Western or traditional Chinese medicines. This study aims to provide the mechanistic insights into the detoxifying effects of Dai antidotes, providing a modern scientific basis for understanding and developing Dai medicine as well as encouraging its use in other regions.Methods: We successively extracted the three Dai antidotes using petroleum ether, ethyl acetate, n-butanol, or water, and prepared their decoctions. Their contents of total flavonoids and polyphenols were determined by colorimetric method. Their antioxidant activity were tested by 1,1-diphenyl-2-picrylhydrazyl(DPPH), hydroxyl radical (·OH) and 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals scavenging assays. Anti-bacterial activity were evaluated in terms of minimum inhibitory concentrations (MICs), minimum bactericidal concentrations (MBCs) and zones of inhibition (ZOI) against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Their anti-inflammatory activity were studied in macrophages stimulated with lipopolysaccharide (LPS) through measuring the production of nitric oxide, tumor necrosis factor-α, interleukin-1β, and interleukin-6.Results: The content of total flavonoids in three Dai antidotes ranged from 22.41 to 586.39 mg/g, which is higher than the content of total polyphenols (2.76 to 28.66 mg/g). The various extracts were found to scavenge radicals of DPPH, ·OH and ABTS. They scavenged ABTS radicals much more efficiently (IC50 9.54 μg/mL) than other radicals (IC50 > 380 μg/mL). They weakly inhibited the growth of E. coli, P. aeruginosa and S. aureus. Notably, even at low concentration 60 μg/mL, the extracts can significantly down-regulate nitric oxide production, tumor necrosis factor-α, interleukin-1β, and interleukin-6 by macrophages stimulated with lipopolysaccharide. Conclusion: In conclusion, our results provide the first mechanistic insights into the detoxifying effects of three Dai antidotes, providing a basis for their optimization and for future research to strengthen Dai medicine through modern scientific practices.

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Flavonoids analysis and antioxidant, antimicrobial, and anti-inflammatory activities of crude and purified extracts from Veronicastrum latifolium

Cited by 32 publications

References 35 publications

Antioxidant Activities of Bingöl Royal Jelly on SH-SY5Y Cells

Antioxidant Activities of Bingöl Royal Jelly on SH-SY5Y Cells

Extraction, Radical Scavenging Activities, and Chemical Composition Identification of Flavonoids from Sunflower (Helianthus annuus L.) Receptacles

Study On Detoxification Mechanism of Three Traditional Dai Antidotes

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