Flavonoids and phenylethanoid glycosides from Lippia nodiflora as promising antihyperuricemic agents and elucidation of their mechanism of action

Cheng, Lee-Chuen; Murugaiyah, Vikneswaran; Chan, Kit‐Lam

doi:10.1016/j.jep.2015.11.025

Cited by 38 publications

(23 citation statements)

References 34 publications

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“…In the present study, potassium oxonate, a well-known uricase inhibitor, was used to induce hyperuricemia in rats [19]. The serum UA level of chemically induced hyperuricemic rats was significantly higher (p<0.01) than normal control, which indicated that hyperuricemic rat model has been successfully established.…”

Section: Antihyperuricemic Activity Of Subfractions E1-e7mentioning

confidence: 68%

Constituent and Antihyperuricemic Activity of Stelechocarpus Burahol Leaves Subfractions

Sunarni

Fidrianny²,

Iwo³

et al. 2017

Asian J Pharm Clin Res

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Objective: The goal of this research was to evaluate antihyperuricemic activity of Stelechocarpus burahol leaves subfractions and isolate its chemical constituent of active subfraction.Methods: Ethyl acetate fraction from S. burahol extract was subfractionated by vacuum liquid chromatography, and the active subfractions were further subfractionated by classic column chromatography using isocratic eluent, followed by isolated chemical constituent from active subfraction. Hyperuricemic rat model was induced by given potassium oxonate intraperitoneally. The inhibitory effect of subfractions on the xanthine oxidase (XO) activity was determined using ultraviolet-visible spectrophotometry method.Results: Subfractions E.3, E.4, and E.5 significantly (p<0.05) reduced the serum uric acid (UA) level 43%, 46%, and 33%, respectively. The E.3, E.4, and E.5 have showed very weak XO inhibitory activity. Subfraction E.3.2 and E.4.3 significantly (p<0.05) reduced the serum UA level 29% and 38%, respectively, however still very weak effect on XO activity. Chemical constituent which was isolated from subfraction E.4.3 was kaempferol-3-O-rhamnoside. Conclusion:The subfractions of ethyl acetate fraction had antihyperuricemic activity in vivo but less effect on XO activity in vitro. Isolated compound in active antihyperuricemic of subfraction E.4.3 was kaempferol-3-O-rhamnoside.

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Section: Antihyperuricemic Activity Of Subfractions E1-e7mentioning

confidence: 68%

Constituent and Antihyperuricemic Activity of Stelechocarpus Burahol Leaves Subfractions

Sunarni

Fidrianny²,

Iwo³

et al. 2017

Asian J Pharm Clin Res

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“…The extraction recovery value of each compound was calculated as a percentage of concentration of the compound spiked into a pulverized plant matrix obtained after extraction over that of an equivalent amount without extraction. The whole plant of L. nodiflora methanolic extract and its fractions F1-F4 were obtained as described previously [9]. For the different plant parts, the dried powder of each (100 mg) was macerated with MeOH at room temperature accordingly.…”

Section: Methods Validationmentioning

confidence: 99%

“…Five bioactive standards, comprising two phenylethanoid glycosides (1,2) and three flavonoids (3-5) isolated from the L. nodiflora following the protocol described previously [9] were used as the external markers for quantification in the plant samples. The purity of the compounds was determined using an Agilent 1120 Compact LC system equipped with a variable wavelength photometric detector and an Agilent EZ-Chrom Elite Compact Software (Agilent Technologies, Santa Clara, California, USA).…”

Section: Preparation Of Calibration Standardsmentioning

confidence: 99%

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Developing a Validated HPLC Method for the Phytochemical Analysis of Antihyperuricemic Phenylethanoid Glycosides and Flavonoids in Lippia nodiflora

Cheng

Murugaiyah

Chan

2017

Natural Product Communications

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The phenylethanoid glycosides and flavonoids of Lippia nodiflora were reported as xanthine oxidase inhibitors that possessed antihyperuricemic effects in chemically-induced hyperuricemic rats. The present study developed a validated high performance liquid chromatography method for the simultaneous phytochemical analysis of five isolated antihyperuricemic constituents in L. nodiflora, comprising two phenylethanoid glycosides, arenarioside (1) and verbascoside (2), and three flavonoids, 6-hydroxyluteolin (3), 6-hydroxyluteolin-7-O-glycoside (4) and nodifloretin (5). These compounds were analysed at 340 nm, using gradient elution of 0.1 % aqueous acetic acid and acetonitrile. The limits of detection were 78.1 ng/mL for 3 and 39.1 ng/mL for the other compounds at a signal-to-noise ratio of 3. The limits of quantification were 312.5 ng/mL for 3 and 156.3 ng/mL for the rest at a signal-to-noise ratio of 12. The intra-day and inter-day accuracy for the five bioactive constituents were between 98.60 % and 100.97 % while their precision values were below 2.11 %. The method was then successfully applied for the quantification of 1-5 in the methanolic extract of L. nodiflora whole plant and its fractions (F1-F4), the methanolic plant part extracts (leaves, stems, roots, and flowers), and water extract of the L. nodiflora whole plant at room temperature.

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“…In addition, our previous study indicated that phenyethanoid glycosides and flavonoids of L. nodiflora were promising xanthine oxidase inhibitors [18]. Two phenylethanoid glycosides, namely, arenarioside (1) and verbascoside (2), together with three flavonoids, namely, 6-hydroxyluteolin (3), 6-hydroxyluteolin-7-O-glycoside (4), and nodifloretin (5), isolated from L. nodiflora were shown to have an antihyperuricemic effect in potassium oxonate-and hypoxanthine-induced hyperuricemic rats, with 3 being the most active [19]. Due to the promising uric acid-lowering benefit of the phenylethanoid glycosides and flavonoids reported previously [18,19], the characterization of their bioavailability and pharmacokinetic properties is important for their further development into phytopharmaceuticals.…”

Section: Introductionmentioning

confidence: 99%

Development, Validation, and Application of HPLC Method for Quantification of Antihyperuricemic Compounds from Lippia nodiflora in Rat Plasma

Cheng

Murugaiyah

Chan

2019

PMIO

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An HPLC method for simultaneous determination of arenarioside (1 ), verbascoside (2), 6-hydroxyluteolin (3), 6-hydroxyluteolin-7-O-glycoside (4), and nodifloretin (5) from Lippia nodiflora in rat plasma was developed and validated. The optimal chromatographic separation was achieved with a gradient mobile phase comprising 0.1% aqueous acetic acid and acetonitrile. The limit of detection was 78.1 ng/mL for 3 and 39.1 ng/mL for the other compounds (signal-to-noise ratio=3), whereas the limit of quantification was 312.5 ng/mL for 3 and 156.3 ng/mL for the other compounds (signal-to-noise ratio=12). The recovery values of compounds 1–5 ranged from 89.37–100.92%. Their accuracy values were between 96.48 and 105.81%, while their corresponding precision values were in the range of 0.75–9.06% for both intraday and inter-day analysis. The method was then applied in the first pharmacokinetic study of 1–5. Following intravenous administration, 1–5 were eliminated slowly from the body with a mean clearance value of 0.11, 0.13, 0.30, 0.09, and 0.23 L/kg h, respectively. Meanwhile, their peak plasma concentration upon oral administration was 8.97, 1.07, 1.06, 0.65, and 0.38 µg/mL, respectively. Compound 3 (5.97%) exhibited the highest absolute oral bioavailability value, followed by 1 (5.22%), 4 (3.13%), 2 (2.10%), and 5 (0.93%).

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Flavonoids and phenylethanoid glycosides from Lippia nodiflora as promising antihyperuricemic agents and elucidation of their mechanism of action

Cited by 38 publications

References 34 publications

Constituent and Antihyperuricemic Activity of Stelechocarpus Burahol Leaves Subfractions

Constituent and Antihyperuricemic Activity of Stelechocarpus Burahol Leaves Subfractions

Developing a Validated HPLC Method for the Phytochemical Analysis of Antihyperuricemic Phenylethanoid Glycosides and Flavonoids in Lippia nodiflora

Development, Validation, and Application of HPLC Method for Quantification of Antihyperuricemic Compounds from Lippia nodiflora in Rat Plasma

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