Floral traits and isolation of three sympatric Aquilegia species in the Qinling Mountains, China

Tang, Lulu; Qian, Yu; Sun, Ji‐Fan; Huang, Shuaiqin

doi:10.1007/s00606-007-0574-6

Cited by 21 publications

(27 citation statements)

References 31 publications

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“…The loss of spur and nectar might produce different fitness and attract different pollinators, which provides the possibility for changes in natural selection. Previous studies have investigated the mating system, floral traits, and habitats of A. ecalcarata , A. incurvata , and A. yabeana in the Qinling Mountains and reported that isolation through differential pollinators and habitats contributed to the reproductive isolation among them (Yu & Huang, ; Tang et al, ). This is good evidence for our hypothesis of changes in natural selection during the divergence of A. ecalcarata .…”

Section: Discussionmentioning

confidence: 99%

“…and A. rockii Munz comprised a monophyletic group (bootstrap value, 97), suggesting that the three species could be sister taxa (Fior et al, ). Another study investigated the mating system, floral traits, and habitats of A. ecalcarata , A. incurvata P. K. Hsiao, and A. yabeana in the Qinling Mountains and proved that isolation through differential pollinators and habitats may contribute to the reproductive isolation among them (Yu & Huang, ; Tang et al, ). Given that A. ecalcarata harbors unique phenotypes in spur and nectar, we regard A. ecalcarata and its closely related species ( A. yabeana , A. oxysepala var.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity and evolutionary history of four closely related Aquilegia species revealed by 10 nuclear gene fragments

Huang

Geng

Fan

et al. 2018

J of Sytematics Evolution

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The genus Aquilegia is emerging as the new model system for plant development, ecology, and evolution studies. Previous research showed that pollinator shift might drive the diversification of North American Aquilegia species, and natural selection on the length of petal nectar spur might play a crucial role. In this genus, A. ecalcarata Maxim. is the only taxon that has lost nectar spurs. Previous phylogenetic results indicated that A. ecalcarata, A. yabeana Kitag., A. oxysepala var. kansuensis Bruhl., and A. rockii Munz comprised a monophyletic group. However, their pattern of genetic diversity remains unknown. In addition, little is known about the evolutionary relationship among the four species on the population level. We carried out a population genetics study with 21 representative populations based on 10 single‐copy nuclear gene fragments and found that: (i) A. yabeana conserved the highest genetic diversity (both πsil and θsil) and A. oxysepala var. kansuensis had the lowest level; (ii) A. ecalcarata split into two groups, with one population clustered with A. rockii and the other five populations clustered with A. oxysepala var. kansuensis; and (iii) the allele frequency spectrum showed an excess of low frequency alleles in all four species, implying that they may undergo the mutation‐drift equilibrium. Our findings provide the first investigation of genetic diversity and evolutionary relationships in A. yabeana, A. oxysepala var. kansuensis, A. rockii, and A. ecalcarata. They lay the foundation for future evolutionary studies, such as speciation mediated by pollinators.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic diversity and evolutionary history of four closely related Aquilegia species revealed by 10 nuclear gene fragments

Huang

Geng

Fan

et al. 2018

J of Sytematics Evolution

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“…Although evolutionary relationships in the genus Aquilegia have been analyzed (Grant, 1992;Hodges and Arnold, 1994;Hodges et al, 2002;Whittall and Hodges, 2007;Hodges and Derieg, 2009) and a significant amount of information of floral traits influencing reproductive isolation is available (Brunet and Eckert, 1998;Medrano et al, 2006;Canto et al, 2007;Tang et al, 2007), little information is available on the extent of genetic variation in commercially cultivated columbine and the wild species (Bolaric et al, 2005). Information on genetic variation in Aquilegia germplasm could also be used to assist genetic mapping, quantitative trait locus (QTL) studies and the design of linkage disequilibrium mapping strategies for marker-trait association (George et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of Aquilegia (Ranunculaceae) species and cultivars assessed by AFLPs

Zhu¹,

Gao²,

Xu³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Species of the genus Aquilegia are exceptionally diverse in their floral morphology and color, commonly known as columbine. They are widely planted ornamentals and are highly attractive for hummingbirds. However, little is known about their genetic diversity. We examined the genetic diversity of the species and cultivars using amplified fragment length polymorphism (AFLP) markers. Sixteen EcoRI/MseI AFLP primer combinations produced 327 informative polymorphic bands, with a mean of 20.4 bands scored per primer. Jaccard's coefficient of similarity varied from 0.61 to 0.93, indicative of high levels of genetic variation. Cluster analysis using the unweighted pair group method with arithmetic mean algorithm placed the 64 accessions into two main clusters, each divided into two sub-clusters. The AFLP variability was significantly associated with the geographic origins, as the Asian species and the North American species grouped into two distinct clusters. The genetic diversity found among Aquilegia demonstrated the potential value of Chinese germplasm for cultivar improvement and for widening the genetic basis of breeding programs and breeding material selection. We concluded that AFLPs are informative and can provide significant insights for genetic diversity research in columbine species.

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“…, Tang et al. ), and many traits known to influence reproductive isolation have been identified (Hodges et al. , Medrano et al.…”

mentioning

confidence: 99%

“…Many resources exist to facilitate the research of this genus, including genetic diversity of species and cultivars (Zhu et al 2011), and genetic maps of several major quantitative trait loci (QTL) (Hodges et al 2002). Moreover, a significant amount of information is available on floral traits that influence reproductive isolation (Brunet and Eckert 1998, Medrano et al 2006, Azucena et al 2007, Tang et al 1998, and many traits known to influence reproductive isolation have been identified (Hodges et al 2002, Medrano et al 2006.…”

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confidence: 99%

Quantitative trait locus mapping of floral and related traits using an F₂ population of Aquilegia

2013

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The objective of this study was to determine quantitative trait loci (QTL) underlying ten floral and related traits in Aquilegia. The traits assessed were calyx diameter, corolla diameter, petal length, petal blade length, sepal length, sepal width, spur length, spur width, plant height and flower number. These are important traits for ornamental value and reproductive isolation of Aquilegia. QTL analysis of these traits was conducted using single‐marker analysis and composite interval mapping (CIM). We used an F2 population consisting of 148 individuals derived from a cross between the Chinese wild species Aquilegia oxysepala and the cultivar Aquilegia flabellata ‘pumila’. Resulting CIM analysis identified 39 QTLs associated with these traits, which were mapped on seven linkage groups. These QTLs could explain 1.22–53.28% of the phenotypic variance. Thirty‐one QTLs, which explained more than 10% of the phenotypic variation, were classified as major QTLs. Graphical representations of the QTLs on seven linkage groups were made. Our research provides the potential for future molecular assisted selection breeding programmes and the cloning of target genes through fine mapping.

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Floral traits and isolation of three sympatric Aquilegia species in the Qinling Mountains, China

Cited by 21 publications

References 31 publications

Genetic diversity and evolutionary history of four closely related Aquilegia species revealed by 10 nuclear gene fragments

Genetic diversity and evolutionary history of four closely related Aquilegia species revealed by 10 nuclear gene fragments

Genetic diversity of Aquilegia (Ranunculaceae) species and cultivars assessed by AFLPs

Quantitative trait locus mapping of floral and related traits using an F₂ population of Aquilegia

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Floral traits and isolation of three sympatric Aquilegia species in the Qinling Mountains, China

Cited by 21 publications

References 31 publications

Genetic diversity and evolutionary history of four closely related Aquilegia species revealed by 10 nuclear gene fragments

Genetic diversity and evolutionary history of four closely related Aquilegia species revealed by 10 nuclear gene fragments

Genetic diversity of Aquilegia (Ranunculaceae) species and cultivars assessed by AFLPs

Quantitative trait locus mapping of floral and related traits using an F2 population of Aquilegia

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Quantitative trait locus mapping of floral and related traits using an F₂ population of Aquilegia