Flow cytometric cell cycle analysis allows for rapid screening of estrogenicity in MCF-7 breast cancer cells

Endocrine disruption and reproductive impairment in zebrafish by exposure to 8:2 fluorotelomer alcohol

“…The present study demonstrated that waterborne exposure to 8:2 FTOH significantly disturbed plasma T and E2 levels in female and male zebrafish, which supported the findings that FTOHs disrupt endocrine homeostasis in previous studies in vitro Vanparys et al, 2006;Liu et al, 2007) and in vivo (Ishibashi et al, 2008;Liu et al, 2009). However, the impact of FTOHs on fish reproduction remains largely unknown.…”

“…Various studies have investigated endocrine disruption, such as those of Maras et al (2006) and Vanparys et al (2006) who reported the estrogen-like properties of 6:2 FTOH and 8:2 FTOH in human breast cancer cells (MCF-7). The estrogenic activities were further demonstrated in primary cultured tilapia hepatocytes (Liu et al, 2007) and in Japanese medaka (Ishibashi et al, 2008).…”

Waterborne exposure to fluorotelomer alcohol 6:2 FTOH alters plasma sex hormone and gene transcription in the hypothalamic–pituitary–gonadal (HPG) axis of zebrafish

“…For instance, exposure of mouse neonates to 8:2 FTOH caused developmental toxicity (Henderson and Smith, 2007). By using cultured human MCF-7 breast cancer cells, 6:2 FTOH and 8:2 FTOH have shown estrogen-like properties Vanparys et al, 2006). The estrogenic activities were further confirmed in primary cultured tilapia hepatocytes and the mechanism may be mediated by estrogenic receptors (ER) (Liu et al, 2007).…”

Per- and polyfluorinated substances in the Nordic Countries

“…Fluorotelomer alcohols have been shown to exert estrogenic activity in MCF-7 cells, in a yeast two-hybrid assay and in aquatic organisms where 6:2 FTOH has been characterised as a stronger xenoestrogen than 8:2 FTOH (Ishibashi et al, 2008;Maras et al, 2006;Vanparys et al, 2006;Wang et al, 2012). According to Ishibashi et al (2007) treatment with 6:2 FTOH, 8:2 FTOH and NFDH (nonadecafluoro-1-decanol) show a dose-dependent interaction with the human estrogen receptor (hER), and rank the estrogenic effects for hERα and hERβ in the descending order of 17β-estradiol>>> 6:2 FTOH > NFDH > 8:2 FTOH.…”