Flow cytometry in oceanography 1989-1999: Environmental challenges and research trends

Legendre, Louis; Courties, Claude; Troussellier, Marc

doi:10.1002/1097-0320(20010701)44:3<164::aid-cyto1108>3.0.co;2-6

Cited by 50 publications

(31 citation statements)

References 87 publications

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“…Flow cytometry is commonly used in immunology and pathology for the diagnosis of diseases and as a research tool for the biomedical sciences. The technique has also found a use in the environmental sciences, including the use of in situ flow cytometers to monitor environmental levels of phytoplankton and bacterioplankton [6].…”

Section: Introductionmentioning

confidence: 99%

Flow cytometric analysis and optimisation for measuring phagocytosis in three Australian freshwater fish

Harford

O’Halloran

Wright

2006

Fish & Shellfish Immunology

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Section: Introductionmentioning

confidence: 99%

Flow cytometric analysis and optimisation for measuring phagocytosis in three Australian freshwater fish

Harford

O’Halloran

Wright

2006

Fish & Shellfish Immunology

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“…When measuring toxicity effects in addition to assessing changes in phytoplankton abundance, physiological properties of cells such as pigment content and cell size can be used to assess potential shifts in community structure linked to an environmental stressor (Legendre et al 2001). Chlorophyll a is a good indicator of the physiological state .…”

Section: Discussionmentioning

confidence: 99%

Toxicity of copper in natural marine picoplankton populations

et al. 2009

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Standard microalgae toxicity tests should be able to establish responses in real ecosystems. Natural marine picoplankton samples collected during the months of March, June, August, October 2007 and January 2008, where exposed to 72 h copper toxicity tests. Results analysed by flow cytometry distinguished two groups, with different cytometric characteristics that can match with two of Synechococcus populations. EC(50) values for these two populations resulted low, ranging from 0.62 to 26.28 microg L(-1), this converts copper in a very powerful contaminant and Synechococcus in one of the most sensitive groups of phytoplankton. Differences in EC(50) values for a same population can be related to the month of collection including different initial cellular densities and oceanographic parameters that can affect the picoplankton's tolerance and distribution.

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“…IFCM was chosen because of the availability of the highly specific monoclonal antibody previously produced for the ELISA method (7) and because flow cytometry has become a common approach for assessing assemblages of minute phytoplankton in marine and freshwater ecosystems (31,40). Additionally, flow cytometry facilitates identification of cells based on multiple parameters simultaneously, allowing for the combination of species-specific identifiers, whether molecular or immunological, and more basic morphological characteristics to accurately determine cell abundance levels.…”

Section: Discussionmentioning

confidence: 99%

Immunofluorescence Flow Cytometry Technique for Enumeration of the Brown-Tide Alga, Aureococcus anophagefferens

Stauffer

Schaffner

Wazniak

et al. 2008

Appl Environ Microbiol

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A new immunologically based flow cytometry (IFCM) technique was developed to enumerate Aureococcus anophagefferens, a small pelagophyte alga that is the cause of "brown tides" in bays and estuaries of the mid-Atlantic states along the U.S. coast. The method utilizes a monoclonal antibody conjugated to fluorescein isothiocyanate (FITC-MAb) to label the surface of A. anophagefferens cells which are then detected and enumerated by using a flow cytometer. Optimal conditions for FITC-MAb staining, including solution composition, incubation times, and FITC-MAb concentrations, were determined. The FITC-MAb method was tested for cross-reactivity with nontarget, similarly sized, photoautotrophic protists, and the method was compared to an enzyme-linked immunosorbent assay (ELISA) using the same MAb. Comparisons of the IFCM technique to traditional microscopy enumeration of cultures and spiked environmental samples showed consistent agreement over several orders of magnitude (r 2 > 0.99). Comparisons of the IFCM and ELISA techniques for enumerating cells from a predation experiment showed a substantial overestimation (up to 10 times higher) of the ELISA in the presence of consumers of A. anophagefferens, presumably due to egested cell fragments that retained antigenicity, using the ELISA method, but were not characterized as whole algal cells by the IFCM method. Application of the IFCM method to environmental "brown-tide" samples taken from the coastal bays of Maryland demonstrated its efficacy in resolving A. anophagefferens abundance levels throughout the course of a bloom and over a large range of abundance values. IFCM counts of the brown-tide alga from natural samples were consistently lower than those obtained using the ELISA method and were equivalent to those of the polyclonal immunofluorescence microscopy technique, since both methods discriminate intact cells. Overall, the IFCM approach was an accurate and relatively simple technique for the rapid enumeration of A. anophagefferens in natural samples over a wide range of abundance values (10 3 to 10 6 cells ml ؊1 ).

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Flow cytometry in oceanography 1989-1999: Environmental challenges and research trends

Cited by 50 publications

References 87 publications

Flow cytometric analysis and optimisation for measuring phagocytosis in three Australian freshwater fish

Flow cytometric analysis and optimisation for measuring phagocytosis in three Australian freshwater fish

Toxicity of copper in natural marine picoplankton populations

Immunofluorescence Flow Cytometry Technique for Enumeration of the Brown-Tide Alga, Aureococcus anophagefferens

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