Follicular right shift: Xenografting queens' ovarian tissue into severe combined imunnodeficiency mice and its responses to exogenous gonadotropin

Santos, Fernanda Araújo dos; Pimentel, Muriel Magda Lustosa; Teixeira, Ana Carolina Guimarães; Cosmo, Ícaro Coutinho; Lima, Mikael Almeida; Brito, Parmênedes Dias de; Macedo, Michelly Fernandes de; Bezerra, Marcelo Barbosa

doi:10.1016/j.theriogenology.2016.05.034

Cited by 7 publications

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“…Some individuals may find themselves in situations of vulnerability such as prey or hunting due to anthropic action. Investigations with xenotransplantations were performed in domestic mammals ( Gosden et al, 1994 ; Barros et al, 2014 ; Tahaei et al, 2015 ; Santos et al, 2016 ) and wild ones ( Wiedemann et al, 2012 ; Praxedes et al, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…The kinetics of follicular development were evidenced when follicular proportions were observed between the different treatments and lineages, with a follicular right shift being observed. A phenomenon characterized by a decrease in the number of primordial follicles and a gradual increase of primary, secondary and antral follicles due to follicle activation and follicular growth after xenotransplantation ( Santos et al, 2016 ), showing that in vivo culture by fetal goat ovary xenotransplantation was efficient and allowed for normal follicular development.…”

Section: Discussionmentioning

confidence: 99%

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Rescue of caprine fetal ovaries, vitrification and follicular development after xenotransplantation in two immunodeficient mice models

et al. 2020

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Domestic and wild goats are very susceptible animals to predation, specially when pregnancy occurs. This study aimed to evaluate the use of goat fetal ovarian tissue for vitrification followed by xenotransplantation and fresh xenotransplantation in two immunosuppressed mice models (C57BL/6 SCID and Balb-C NUDE). Goat fetus ovaries were collected in slaughterhouses, divided into small cortical pieces and were destined for fresh xenotransplantation (FX) and cryopreservation followed by xenotransplantation (CX). Five recipients from each lineage were used for FX and 10 animals from each lineage for CX. The mice were euthanized after 65 postoperative days, and the transplants were collected for microscopic assessment. The blood plasma was collected for estradiol measurement. Independently of mice strain, all recipients presented complete estrus cycle in FX and 80% after CX groups. Follicles were observed at all development stages without morphological changes. The volume density and total vessel surface observed in the transplants were different (p <0.01) between groups. The estradiol levels in the recipients did not differ (p <0.05) among the treatments. Thus, it is possible to activate the preantral follicles in the ovaries of fetuses by optimizing germplasm utilization and conservation of domestic and endangered wild goats that are in predatory situations, undesirable drowning or accidental death, since provided conditions for xenotransplantation are performed.

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