Free radical-scavenging capacity, antioxidant activity and phenolic content of Pouzolzia zeylanica

Li, Peiyuan; Huo, Lini; Su, Wei; Lu, Rumei; Deng, Chaocheng; Liu, Liangquan; Deng, Yongkun; Guo, Nana; Cheng-sheng, LU; He, Chunling

doi:10.2298/jsc100818063l

Cited by 89 publications

(58 citation statements)

References 21 publications

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“…ABTS radical scavenging activity was measured by the method described by Li et al [43]. For this, 10 μ L of the sample was added to 4 mL of the diluted ABTS ∙+ solution (prepared by adding 7 mM of the ABTS stock solution to 2.45 mM potassium persulfate, kept in the dark, at room temperature, for 12–16 h before use).…”

Section: Methodsmentioning

confidence: 99%

Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture ofCordyceps gracilis(Grev.) Durieu & Mont.

Sharma

Gautam

Atri

2015

Evidence-Based Complementary and Alternative Medicine

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Under present experiments, EPS and IPS production, monosaccharide composition, and antioxidant activities of C. gracilis were studied for the first time under submerged culture conditions. Effect of different factors on polysaccharides production was studied by orthogonal experiments using one-factor-at-a-time method. Incubation of culture in the medium with capacity 200 mL (675.12 ± 5.01 and 385.20 ± 5.01 mg/L), rotation speed 150 rpm (324.62 ± 3.32 and 254.62 ± 4.62 mg/L), 6-day culture incubation time (445.24 ± 1.11, 216.60 ± 1.71 mg/L), pH 6.0 (374.81 ± 2.52 and 219.45 ± 2.59 mg/L), and temperature 23°C (405.24 ± 1.11 and 215.60 ± 1.71 mg/L) produced higher EPS and IPS, respectively. Maximum EPS and IPS production was observed in the medium supplemented with glucose as a carbon source (464.82 ± 2.12 and 264.42 ± 2.62 mg/L) and yeast extract as a nitrogen source (465.21 ± 3.11 and 245.17 ± 3.24 mg/L), respectively. Carbon to nitrogen ratio for maximum EPS and IPS production was observed as 10 : 1 (395.29 ± 2.15 and 235.62 ± 1.40 mg/L), respectively. Glucose was found to be the major monosaccharide (62.15 ± 7.33%). Both EPS and IPS of C. gracilis showed significant DPPH radical scavenging activity, ABTS radical scavenging activity, reducing power, and iron chelating activity.

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Section: Methodsmentioning

confidence: 99%

Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture ofCordyceps gracilis(Grev.) Durieu & Mont.

Sharma

Gautam

Atri

2015

Evidence-Based Complementary and Alternative Medicine

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“…ABTS (2, 2'-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid) free radical scavenging activity was analysed according to the method described by Li, et al 2011 23 with moderate modifications. Briefly, ABTS•+ stock solution was prepared by mixing 7 mM of ABTS and 2.45 mM of potassium persulfate and incubation at room temperature for 12-16 h. The ABTS • + working solution was prepared by dilution of the ABTS•+ stock solution and distilled water to gain a 0.70±0.02 absorbance at 734 nm.…”

Section: Abts Radical Scavenging Assaymentioning

confidence: 99%

Green Synthesis of Gold Nanoparticles Using Sumac Aqueous Extract and Their Antioxidant Activity

et al. 2016

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Green synthesis of nanoparticles which have eco-friendly favourable solvent systems and environmentally reducing agents is of excessive importance. In this paper, we aimed to develop environmentally friendly, rapid and simple producer for the synthesis gold nanoparticles (Au-NPs) using aqueous extract of sumac as reducing agents for gold ions as well capping agent for the bioformed Au-NPs. The bio-synthesized Au-NPs were characterized by the UV-visible spectroscopy, FTIR, TEM, and zeta-potential measurements. The surface plasmon resonance band centred at 520 nm for Au-NPs was characterized by UV-visible spectrophotometer. The probable bio-molecules are polyphenols may responsible for reduction of gold ions were recognized through FT-IR. The TEM result shows the bioformed Au-NPs are spherical in shapes with the mean size of 20.83 ±4.4 nm. The capping of anionic bio-molecules on the surface of Au-NPs was confirmed by zeta potential assessment (-25.3 mV) and is responsible for the electrostatic stability. In vitro antioxidant activity studies showed that DPPH (2,2-diphenyl-1-picrylhydrazyl) and ABTS (2, 2'-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid) activities increased in a dose dependent manner. The bio-synthesized nanoparticles can potentially useful in pharmaceutical and biomedical applications.

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“…Análises de atividade antioxidante, utilizando os radicais DPPH, ABTS, e ensaio usando a cooxidação do β-caroteno frente ao ácido linoleico têm sido muito utilizados para verificar a capacidade sequestradora de radicais livres de muitos produtos naturais (LI et al, 2011;ALVES et al, 2010).…”

Section: Resultsunclassified

Atividade antioxidante e antimicrobiana de extratos de atemoia (Annona cherimola Mill. x A. squamosa L.)

et al. 2014

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RESUMO -O objetivo deste trabalho foi quantificar os teores de fenóis e flavonoides totais, bem como avaliar as atividades antioxidante e antimicrobiana de extratos obtidos dos talos e folhas de atemoia (A. cherimola Mill. x A. squamosa L.), que pertence à família Annonaceae. A atividade antioxidante foi avaliada pelos métodos de sequestro dos radicais 2,2-difenil-1-picrilhidrazil (DPPH) e 2,2´-azinobis-3-etilbenzotiazolina-6-ácido sulfônico (ABTS), bem como pelo método da cooxidação do β-caroteno/ácido linoleico. A avaliação da atividade antimicrobiana dos extratos foi analisada contra 10 cepas de bactérias. Os resultados da atividade antioxidante dos extratos mostraram que o extrato etanólico dos talos (EEt) foi o antioxidante mais efetivo (IC 50 = 10,44 ± 1,25 µg/mL) no método do sequestro do DPPH, bem como no sequestro do radical ABTS (24,81 ± 0,49%). O extrato hexânico das folhas apresentou o melhor percentual de atividade antioxidante no ensaio do β-caroteno/ácido linoleico (41,12 ± 4,35%). Os extratos etanólico dos talos e metanólico das folhas mostraram-se ativos contra cepas de Bacillus cereus, Klebsiella pneumoniae, Staphylococcus aureus resistente à meticilina (MRSA), Staphylococcus aureus e Staphylococcus epidermidis. Termos para indexação: plantas medicinais, atemoia, atividade antibacteriana, radicais livres. ANTIOXIDANT AND ANTIMICROBIAL ACTIVITY OF EXTRACTS FROM ATEMOIA (Annona cherimola Mill. x A. squamosa L.)ABSTRACT -The aim of this study was to quantify the levels of total phenols and flavonoids as well as to evaluate the antioxidant and antimicrobial activities of extracts obtained from stems and leaves of atemoia (A. cherimola Mill. x A. squamosa L.), which belongs to the Annonaceae family. The antioxidant activity was evaluated by using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) scavengers methods, as well as the method of co-oxidation of β-carotene/linoleic acid. The antimicrobial activity of the extracts was assessed against 10 strains of bacteria. The results showed that the antioxidant activity of the ethanolic extract from the stems (EEt) was the most effective antioxidant (IC 50 = 10.44 ± 1.25 mg/ml) by the DPPH method, as well as in the ABTS radical scavenger (24.81 ± 0.49%). The hexane extract of the leaves showed the best percentage of antioxidant activity by β-carotene/linoleic acid test (41.12 ± 4.35%). The ethanolic and metabolic extracts were active against strains of Bacillus cereus, Klebsiella pneumoniae, Methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus aureus and Staphylococcus epidermidis.

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Free radical-scavenging capacity, antioxidant activity and phenolic content of Pouzolzia zeylanica

Cited by 89 publications

References 21 publications

Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture ofCordyceps gracilis(Grev.) Durieu & Mont.

Optimization, Composition, and Antioxidant Activities of Exo- and Intracellular Polysaccharides in Submerged Culture ofCordyceps gracilis(Grev.) Durieu & Mont.

Green Synthesis of Gold Nanoparticles Using Sumac Aqueous Extract and Their Antioxidant Activity

Atividade antioxidante e antimicrobiana de extratos de atemoia (Annona cherimola Mill. x A. squamosa L.)

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