Frequent detection of bovine polyomavirus in commercial batches of calf serum by using the polymerase chain reaction

Schuurman, Rob; Steenis, Bert van; Strien, A. van; Noordaa, J. van der; Sol, C. J. A.

doi:10.1099/0022-1317-72-11-2739

Cited by 32 publications

(17 citation statements)

References 21 publications

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“…For example, BPV was recovered from tissue cultures and is believed to be a contaminant with the calf serum, but no study we are aware of reported its natural host. Some doubt is cast on the bovid origin of BPV by the lack of correlation between detection of viral DNA by PCR and the presence of virus-specific antibodies in serum (51). Similarly, the natural host of the LPV is not known for certain and serological studies have not been able to address this uncertainty.…”

Section: Discussionmentioning

confidence: 99%

Comparing Phylogenetic Codivergence between Polyomaviruses and Their Hosts

Pérez‐Losada

Christensen

McClellan

et al. 2006

J Virol

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Seventy-two full genomes corresponding to nine mammalian (67 strains) and two avian (5 strains) polyomavirus species were analyzed using maximum likelihood and Bayesian methods of phylogenetic inference. Our fully resolved and well-supported (bootstrap proportions > 90%; posterior probabilities ‫؍‬ 1.0) trees separate the bird polyomaviruses (avian polyomavirus and goose hemorrhagic polyomavirus) from the mammalian polyomaviruses, which supports the idea of spitting the genus into two subgenera. Such a split is also consistent with the different viral life strategies of each group. Simian (simian virus 40, simian agent 12 [Sa12], and lymphotropic polyomavirus) and rodent (hamster polyomavirus, mouse polyomavirus, and murine pneumotropic polyomavirus [MPtV]) polyomaviruses did not form monophyletic groups. Using our best hypothesis of polyomavirus evolutionary relationships and established host phylogenies, we performed a cophylogenetic reconciliation analysis of codivergence. Our analyses generated six optimal cophylogenetic scenarios of coevolution, including 12 codivergence events (P < 0.01), suggesting that Polyomaviridae coevolved with their avian and mammal hosts. As individual lineages, our analyses showed evidence of host switching in four terminal branches leading to MPtV, bovine polyomavirus, Sa12, and BK virus, suggesting a combination of vertical and horizontal transfer in the evolutionary history of the polyomaviruses.

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Section: Discussionmentioning

confidence: 99%

Comparing Phylogenetic Codivergence between Polyomaviruses and Their Hosts

Pérez‐Losada

Christensen

McClellan

et al. 2006

J Virol

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“…BPyV sequences have been reported by diverse authors to be found in calf serum batches (24,32,38,39). Although antibodies have been detected in cattle (32,44) and in humans in close contact with cattle (31), no specific illness has been attributed to BPyV.…”

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confidence: 99%

Identification of Human and Animal Adenoviruses and Polyomaviruses for Determination of Sources of Fecal Contamination in the Environment

Hundesa

Motes

Bofill-Mas

et al. 2006

Appl Environ Microbiol

150

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The Adenoviridae and Polyomaviridae families comprise a wide diversity of viruses which may be excreted for long periods in feces or urine. In this study, a preliminary analysis of the prevalence in the environment and the potential usefulness as source-tracking tools of human and animal adenoviruses and polyomaviruses has been developed. Molecular assays based on PCR specifically targeting human adenoviruses (HAdV), porcine adenoviruses (PAdV), bovine adenoviruses (BAdV), and bovine polyomaviruses (BPyV) were applied to environmental samples including urban sewage, slaughterhouse, and river water samples. PAdV and BPyV were detected in a very high percentage of samples potentially affected by either porcine or bovine fecal contamination, respectively. However, BAdV were detected in only one sample, showing a lower prevalence than BPyV in the wastewater samples analyzed. The 22 slaughterhouse samples with fecal contamination of animal origin showed negative results for the presence of HAdV. The river water samples analyzed were positive for the presence of both human and animal adenoviruses and polyomaviruses, indicating the existence of diverse sources of contamination. The identities of the viruses detected were confirmed by analyses of the amplified sequences. All BPyV isolates showed a 97% similarity in nucleotide sequences. This is the first time that PAdV5, BAdV6, and BPyV have been reported to occur in environmental samples. Human and porcine adenoviruses and human and bovine polyomaviruses are proposed as tools for evaluating the presence of viral contamination and for tracking the origin of fecal/urine contamination in environmental samples.

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“…Therefore, BPyV should be considered as one of the tumorigenic polyomaviruses. Since commercial batches of (foetal) calf serum have been shown to be frequently contaminated with this virus (Schuurman et al, 1991 a) it is recommended that cattle sera should be screened for the presence of BPyV prior to use in cell culture.…”

Section: Discussionmentioning

confidence: 99%

“…Foetal calf serum (FCS) used to supplement cell culture media was tested for BPyV prior to use by using the polymerase chain reaction (PCR;Schuurman et al, 1991 a).…”

Section: Methodsmentioning

confidence: 99%