Fructose production by Zymomonas mobilis in fed-batch culture with minimal sorbitol formation

Edye, L. A.; Johns, Michael R.; Ewings, Ken N.

doi:10.1007/bf00262449

Cited by 20 publications

(10 citation statements)

References 8 publications

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“…Mutants of Tricholoma nudum NRRL 2371 (8), Fusarium sp. F5 (3), Pullularia pullulans ATCC 9348 (9), Z. mobilis ATCC 39676 (10), Z. mobilis ATCC 53432 (11), and Z. mobilis ATCC 53431 (5,12,13) were used for the production of fructose form sucrose media. The results with the majority of these microorganisms showed that certain difficulties, such as considerable fructose consumption and/or production of unwanted byproducts such as sorbitol, were encountered.…”

Section: Introductionmentioning

confidence: 99%

Production of Fructose and Ethanol from Sugar Beet Molasses Using Saccharomyces cerevisiae ATCC 36858

Atiyeh

2002

Biotechnology Progress

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The production of enriched fructose syrups and ethanol from beet molasses using Saccharomyces cerevisiae ATCC 36858 was studied. In batch experiments with a total sugar concentration between 94.9 and 312.4 g/L, the fructose yield was above 93% of the theoretical value. The ethanol yield and volumetric productivity in the beet molasses media with sugar concentration below 276.2 g/L were in the range of 59-76% of theoretical value and between 0.48 and 2.97 g of ethanol/(L x h), respectively. The fructose fraction in the carbohydrates content of the produced syrups was more than 95% when the total initial sugar concentration in the medium was below 242.0 g/L. Some oligosaccharides and glycerol were also produced in all tested media. Raffinose and the produced oligosaccharides were completely consumed by the end of the fermentation process when the total initial sugar concentration was below 190.1 g/L. The glycerol concentration was below 16.1 g/L. The results could be useful for a potential industrial production of ethanol and high-fructose syrup from sugar beet molasses.

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Section: Introductionmentioning

confidence: 99%

Production of Fructose and Ethanol from Sugar Beet Molasses Using Saccharomyces cerevisiae ATCC 36858

Atiyeh

2002

Biotechnology Progress

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“…The sucrose hydrolysis efficiencies of S. cerevisiae ATCC 36858 were 98% in a 629 g/l sucrose-containing synthetic medium [1] and 100% in a 312 g/l sucrose-containing sugar beet molasses [2]. The efficiencies with Z. mobilis ranged between 73% and 94% in media containing between 350 and 462 g/l of sucrose [8,11,30]. M. guilliermondii SK-ENNY may have some advantages over these other strains.…”

Section: Production Of Hfgsmentioning

confidence: 98%

A Novel Production Method for High-Fructose Glucose Syrup from Sucrose-Containing Biomass by a Newly Isolated Strain of Osmotolerant Meyerozyma guilliermondii

Khattab¹,

Kodaki²

2016

Journal of Microbiology and Biotechnology

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One osmotolerant strain from among 44 yeast isolates was selected based on its growth abilities in media containing high concentrations of sucrose. This selected strain, named SKENNY, was identified as Meyerozyma guilliermondii by sequencing the internal transcribed spacer regions and partial D1/D2 large-subunit domains of the 26S ribosomal RNA. SK-ENNY was utilized to produce high-fructose glucose syrup (HFGS) from sucrose-containing biomass. Conversion rates to HFGS from 310-610 g/l of pure sucrose and from 75-310 g/l of sugar beet molasses were 73.5-94.1% and 76.2-91.1%, respectively. In the syrups produced, fructose yields were 89.4-100% and 96.5-100% and glucose yields were 57.6-82.5% and 55.3-79.5% of the theoretical values for pure sucrose and molasses sugars, respectively. This is the first report of employing M. guilliermondii for production of HFGS from sucrose-containing biomass.

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“…1. Fructose, ethanol and biomass yields, specific growth rate and specific initial rate of sugar hydrolysis and ethanol productivity in sugar cane molasses and synthetic media using S. A relatively high sorbitol concentration was often noticed with Z. mobilis in sugar cane syrup or synthetic sucrose media [5,[11][12][13]. However, a lower sorbitol production was reported in media with C-molasses.…”

Section: Effects Of Total Sugar Concentrationmentioning

confidence: 99%

Production of Fructose and Ethanol from Cane Molasses Using Saccharomyces cerevisiae ATCC 36858

Atiyeh¹

2003

Acta Biotechnologica

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SummaryThe purpose of this research was to study the possibility of the production of ethanol and enriched fructose syrups from sugar cane molasses using the yeast Saccharomyces cerevisiae ATCC 36858. In batch experiments with a total sugar concentration of between 96.7 g/l and 323.5 g/l, the fructose yield was above 90% of the theoretical value. The ethanol yield and volumetric productivity were in the range of 66% and 77% of the theoretical value, and between 0.53 g ethanol/l × h and 3.15 g ethanol/l × h, respectively. The fructose fraction in the carbohydrates content of the produced syrups was more than 95% when the total initial sugar concentration in the medium was below 273.8 g/l. Some oligosaccharides and glycerol were also produced in all tested media. The maximum amount of produced oligosaccharides including raffinose accounted for 13.4 g/l in the cane molasses medium with 323.5 g/l sugars in the initial phase of the fermentation process. The oligosaccharides produced and raffinose were completely consumed by the end of the fermentation process when the total initial sugar concentration was less than 191.3 g/l. The glycerol concentration was below 9.9 g/l. These findings are useful in the production of ethanol and high fructose syrups using sugar cane molasses.

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Fructose production by Zymomonas mobilis in fed-batch culture with minimal sorbitol formation

Cited by 20 publications

References 8 publications

Production of Fructose and Ethanol from Sugar Beet Molasses Using Saccharomyces cerevisiae ATCC 36858

Production of Fructose and Ethanol from Sugar Beet Molasses Using Saccharomyces cerevisiae ATCC 36858

A Novel Production Method for High-Fructose Glucose Syrup from Sucrose-Containing Biomass by a Newly Isolated Strain of Osmotolerant Meyerozyma guilliermondii

Production of Fructose and Ethanol from Cane Molasses Using Saccharomyces cerevisiae ATCC 36858

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