FTIR analysis of natural and synthetic collagen

Riaz, Tehseen; Zeeshan, Rabia; Zarif, Faiza; Ilyas, Kanwal; Muhammad, Nawshad; Safi, Sher Zaman; Rahim, Abdur; Rizvi, Syed A. A.; Rehman, Ihtesham Ur

doi:10.1080/05704928.2018.1426595

Cited by 423 publications

(239 citation statements)

References 165 publications

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“…Several characteristic peaks of PCL were found at 2920 cm –1 for asymmetric CH 2 stretching, 2865 cm –1 for symmetric CH 2 stretching, 1720 cm –1 for ester carbonyl group, 1290 cm –1 for CO and CC stretching, 1240 cm –1 for asymmetric COC stretching, and 1170 cm –1 for symmetric CO stretching; similar results were obtained by another researcher as well . For collagen, some typical bands were detected around 3300 cm –1 for amide A (NH stretching vibration), 1650 cm –1 for CNH 2 , 1530 cm –1 for CNH and 1240 cm –1 for CN (plane bending of NH and CN stretching vibration) . Regarding the chitosan, the spectrum showed 2 certain features at 1650 and 1600 cm –1 which are attributed to CNH 2 and CN of C=O stretching vibration and NH bending of NH 2 .…”

Section: Resultssupporting

confidence: 86%

Graphene oxide incorporated polycaprolactone/chitosan/collagen electrospun scaffold: Enhanced osteogenic properties for bone tissue engineering

et al. 2019

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This in vitro study aimed to evaluate the physicochemical and biological activity of the polycaprolactone/chitosan/collagen scaffolds incorporated with 0, 0.5, 3, and 6 wt% of graphene oxide (GO). Using standard tests and MG-63 cells, the characteristics of scaffolds were evaluated, and the behavior of osteoblasts were simulated, respectively. A non-significant decrease in nanofibers diameter was noted in scaffolds with a higher ratio of GO. The hydrophilicity and bioactivity of the scaffold surface, as well as cell attachment and proliferation, increased in correspondence to an increase in GO. The higher ratio of GO also improved the osteogenesis activity.GO increased the degradation rate, but it was negligible and seemed not enough to endanger stability. Modifying the scaffolds with GO did not make a significant change to the antibacterial effect.

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Section: Resultssupporting

confidence: 86%

Graphene oxide incorporated polycaprolactone/chitosan/collagen electrospun scaffold: Enhanced osteogenic properties for bone tissue engineering

et al. 2019

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“…Finally, Amide III showed a peak frequency of 1,238 cm −1 in R. Pulmo, compared to 1,240 cm −1 from human placenta and 1,243 cm −1 from rat tail tendon. The absorption intensity of 1,550 cm −1 (amide II) indicated that hydrogen bonding is present (Riaz et al, 2018); while absorption intensity of 1,238 cm-1 (amide III) confirmed that triple helical structure is intact (Riaz et al, 2018). FTIR confirmed the triple helix structure, high extent of intermolecular structure, and similar secondary structure of the proteins between different sources of collagen (Riaz et al, 2018).…”

Section: Fourier-transform Infrared (Atr-ftir) Spectroscopymentioning

confidence: 88%

“…FTIR generates a spectral fingerprint that can provide structural insights into collagen structure based on the presence and intensity of distinct peaks that correspond to amide A/B and amide I, II, and II bonds crucial to the formation of the triple helix (Belbachir et al, 2009;Riaz et al, 2018). The main absorption bands in R. pulmo collagen were amide A (3,283 cm −1 ), amide B (2,934 cm −1 ), amide I (1,647 cm −1 ), amide II (1,550 cm −1 ), and amide III (1,238 cm −1 ), typical bands for collagen type I (Figure 2).…”

Section: Fourier-transform Infrared (Atr-ftir) Spectroscopymentioning

confidence: 99%

Marine Collagen Substrates for 2D and 3D Ovarian Cancer Cell Systems

Paradiso

Fitzgerald

Yao

et al. 2019

Front. Bioeng. Biotechnol.

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A fundamental structural component of extracellular matrix in all connective and interstitial tissue, collagen is the most abundant protein in the human body. To date, mammalian collagens sources represent the golden standard for multiple biomedical applications, while marine-derived collagens have largely been used in industry (food, pharmaceutical, and cosmetic), with little use in research and clinical applications. Herein we demonstrate the effective use Rhizostoma pulmo jellyfish collagen, a source of biocompatible, sustainable collagen for 2D and 3D cell culture, addressing the global drive for technological developments that result in the replacement of animals and their derived products in research. Jellyfish collagen harbors similar structural features mammalian collagen type I, despite differing slightly in amino acid content. Jellyfish collagen supports ovarian cancer (OvCa) cell line proliferation, cellular morphology and expression of epithelial to mesenchymal transition markers, supporting the use of R. pulmo as a non-mammalian collagen cell culture substrate. Furthermore, R. pulmo collagen is effective in 3D device fabrication such as sponges where it mimics tissue architecture complexity. OvCa cells migrated and differentiated within the R. pulmo collagen 3D scaffolds confirming its suitability for advanced cell culturing applications, providing an excellent alternative to mammalian collagen sources for the culture of human cells.

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“…This study presents new collagen-based materials for biomedical applications, especially in dentistry. These new materials, collagen-based spongy materials, obtained by the lyophilization method, were characterized by various techniques like optical microscopy, IR spectroscopy, water [30,31] Hydrogen bond between N-H stretch (ν NH ) and C-O (ν CO ) [29] 1607 1606 1608 1610…”

Section: Resultsmentioning

confidence: 99%

Piroxicam-Collagen-Based Sponges for Medical Applications

Ioan

Rău

Tihan

et al. 2019

International Journal of Polymer Science

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Depending on the concentration of the drug and/or the method of administration, drugs could be used in various ways. To take full advantage of the drug beneficial properties in oral medical interventions but also in other types of surgery, like plastic surgery, general surgery, or gynecological surgery, the drug concentration as well as the administration method itself will depend on the wound, type of surgery, and severity of the postoperative pain which can be very different. Generally, the local administration methods are recommended. Piroxicam, a nonsteroidal anti-inflammatory drug (NSAID) of the oxicam class, is generally used to relieve the symptoms of pain and inflammation. Starting from the idea of the special benefit of the interference between collagen-based materials and drug beneficial properties, our work was focused on the synthesis and characterization of new collagen-piroxicam materials. These new collagen-based materials present a good water absorption, and the piroxicam release suggests a biphasic drug release profile whereas the obtained values for the release exponent revealed a complex release mechanism including swelling, diffusion, and erosion.

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FTIR analysis of natural and synthetic collagen

Cited by 423 publications

References 165 publications

Graphene oxide incorporated polycaprolactone/chitosan/collagen electrospun scaffold: Enhanced osteogenic properties for bone tissue engineering

Graphene oxide incorporated polycaprolactone/chitosan/collagen electrospun scaffold: Enhanced osteogenic properties for bone tissue engineering

Marine Collagen Substrates for 2D and 3D Ovarian Cancer Cell Systems

Piroxicam-Collagen-Based Sponges for Medical Applications

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