Further molecular characterisation of potyviruses infecting aroid plants for medicinal use in China

Shi, Yuhong; Hong, X.-Y.; Chen, J.; Adams, M. J.; Zheng, Hongying; Lin, Le; Qin, Bangyong

doi:10.1007/s00705-004-0390-z

Cited by 25 publications

(6 citation statements)

References 11 publications

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“…ex Breitenb. in China (Chen et al ., 2004) and was later found to occur widely in aroid plants (Shi et al ., 2005). This strain could be mechanically inoculated to some soybean cultivars, in which it induced weak symptoms.…”

Section: Introductionsupporting

confidence: 91%

A potyvirus P1 protein interacts with the Rieske Fe/S protein of its host

Shi

Chen

Hong

et al. 2007

Molecular Plant Pathology

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Yeast two-hybrid (Y2H) screens were used to test for interactions between the P1 protein of Soybean mosaic virus Pinellia isolate (SMV-P) and a cDNA expression library of its host, the aroid Pinellia ternata. Of the 13 independent interacting clones identified, ten were identical and had an open reading frame predicted to encode a 23.7-kDa protein closely related to the cytochrome b6/f complex Rieske Fe/S genes of plants. The interaction between SMV-P-P1 and the mature Rieske Fe/S protein (without transit peptide) of the host was confirmed by in vitro co-immunoprecipitation of the two proteins. Y2H assays using different parts of the two proteins showed that only the N-terminal part (amino acids 1-82) of SMV-P P1 was responsible for the interaction with the Rieske Fe/S protein and that amino acids 1-33 interacted only with the transit peptide, while amino acids 34-82 could interact with the entire Rieske Fe/S protein. SMV-P P1 also interacted moderately with the Rieske Fe/S protein of its other hosts, soybean and Zantedeschia aethiopica, but weakly with that of the non-host Arabidopsis thaliana. The P1-Rieske Fe/S protein interactions are likely to be involved in symptom development, and the very variable N-terminus of P1 may play an important role in host adaptation.

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Section: Introductionsupporting

confidence: 91%

A potyvirus P1 protein interacts with the Rieske Fe/S protein of its host

Shi

Chen

Hong

et al. 2007

Molecular Plant Pathology

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“…KoMV belongs to the family Potyviridae , and infects aroids, including Amorphophallus , Caladium , Colocasia , Dieffenbachia , Philodendron , Typhonium , Zamioculcas and Zantedeschia species (Alexandre, Duarte, Rivas, Kitajima, & Harakava, 2013; Chang, Chen, & Chung, 2001; Manikonda et al, 2011; Padmavathi, Srinivas, Hema, & Sreenivasulu, 2013; Shi et al, 2005; Shimoyama, Kameya‐Iwkai, Hanada, & Gunji, 1992). KoMV‐infected calla lilies show symptoms such as mosaic, green islands, veinal chlorosis on leaves, leaf distortion, short peduncles and discoloured pigmented flecks on the spathes (Chang et al, 2001; Pham et al, 2002).…”

Section: Introductionmentioning

confidence: 99%

“…KoMV infection causes yield losses and decreases the value of the commodity. KoMV has been spread worldwide, including to Brazil, China, Germany, India, Japan, Korea, Taiwan, the Netherlands and New Zealand (Alexandre et al, 2013; Chang et al, 2001; Kwon, Ha, Yoon, & Ryu, 2002; Lesemann & Winter, 2002; Manikonda et al, 2011; Pham et al, 2002; Shi et al, 2005; Shimoyama et al, 1992; Wei, Pearson, Cohen, Tang, & Clover, 2008).…”

Section: Introductionmentioning

confidence: 99%

Vector transmission of konjac mosaic virus to calla lily (Zantedeschia spp.) by aphids

Liao

Mou

Chang

et al. 2020

Annals of Applied Biology

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Various viral diseases and bacterial soft rot are the major limiting factors for the cultivation of calla lilies (Zantedeschia spp.). Konjac mosaic virus (KoMV) is one of the major causal agents of viral diseases in calla lily fields in Taiwan. The objectives of this study are to identify potential insect vectors of KoMV and to assess the biotic and abiotic factors affecting aphid transmission of KoMV to calla lily. In this study, we identified Myzus persicae and Aphis gossypii as potential vectors of KoMV. Early‐instar nymphs, late‐instar nymphs, apterous adults and alate adults of both aphid species were able to transmit KoMV, and no significant differences in the transmission rates were detected. The difference in KoMV transmission rates was not detected when the transmission assays were performed at 18, 23 and 28°C. Transmission biology is a crucial component of plant disease epidemiology caused by viruses. Vector propensity and vector activity need to be further investigated in the field. The knowledge will be valuable for developing disease control strategies for KoMV in calla lily fields.

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“…The genome is 9,544 nucleotides long excluding the 3-terminal polyA tail and encodes a typical potyviral 350 kDa polyprotein of 3,087 amino acids [16]. Some researchers had found that ZaMV could infect calla lily plants in Taiwan, Korea, and Typhomium flagelliforme in China [16][17][18]. Okuno et al [19] had isolated JHMV from Japanese hornwort plants (Cryptotaenia japonica) with mosaic disease symptoms.…”

Section: Introductionmentioning

confidence: 99%

A new potyvirus first isolated and identified from Angelica sinensis

Zhang

Wang

et al. 2009

Virus Genes

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A filamentous virus was isolated in Angelica sinensis (Angelica sinensis (Oliv.) Diels) which shows mosaic symptoms on leaves in Minxian, Gansu province, China. According to morphology and molecular biology properties, this virus, which has a flexuous rod-shaped particle about 750 nm in length and 12 nm in width, was assigned to the genus Potyvirus, family Potyviridae. Its coat protein (CP) shows high similarity with six other potyviruses by analysis of peptide mass fingerprinting (PMF). The 919 bp nucleotides of 3' terminal covering partial CP gene and 3'-untranslated region was amplified by RT-PCR using degenerate primers which were designed according to the result of PMF. In sequence comparisons and phylogenetic analysis, the new isolate was found to be closely related to Japanese hornwort mosaic virus (JHMV), Konjak mosaic virus (KoMV), and Zantedeschia mosaic virus (ZaMV). The most closely related virus is JHMV03 (AB251346), with 96.59% aa and 87.60% nt identity to the isolate. All results suggest the presence of a new member of potyvirus, tentatively named Dang Gui strain of Japanese hornwort mosaic virus (JHMV-DG*). In our research the antiserum against the CP of JHMV-DG had also been prepared. To our knowledge, it is the first time that a potyvirus has been isolated and identified in Angelica sinensis.

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Further molecular characterisation of potyviruses infecting aroid plants for medicinal use in China

Cited by 25 publications

References 11 publications

A potyvirus P1 protein interacts with the Rieske Fe/S protein of its host

A potyvirus P1 protein interacts with the Rieske Fe/S protein of its host

Vector transmission of konjac mosaic virus to calla lily (Zantedeschia spp.) by aphids

A new potyvirus first isolated and identified from Angelica sinensis

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