Further Studies on Use of Polymyxin B Sulfate with Dihydrostreptomycin and Penicillin for Control of Vibrio fetus in a Frozen Semen Process

Influence of Two Extension Procedures on the Ubiquitous Microflora and the Biological Quality of Frozen Bovine Semen

“…One of the main reasons for the tremendous success of bovine artificial insemination lies in its efficacy in controlling the dissemination of diseases whose pathogen agents are potentially born by semen. The best procedures for controlling these pathogens have been extensively studied both in terms of environmental conditions (Bartlett et al 1976;Schultz 1977;Hafs 1978;Lyle 1978;Riek and Pickett 1980) and in terms of efficiency of antibiotics (Sullivan et al 1966 . 1980;Hare 1985;Shin and Lein 1986;Shin et al 1988;Fernandez-Reguena et al 1994).…”

Effect of Thawing Procedures on Fertility of Bovine Spermatozoa Frozen in .25-ml Straws

“…Only ejaculates containing at least 50% motile spermatozoa were utilized. Semen for Trials 1, 2, 3, 6, 7 and 8 was treated with antibiotics prior to dilution (Sullivan et al, 1966;Ennen et al, 1976), while antibiotics were added to the semen for Trials 4 and 5 by inclusion in the extender (Ennen et al, 1976). Semen was diluted at 37 C with one volume of 20% egg yolk-2.9% sodium citrate (EYC) extender and cooled to 5 C in 4 hr (Ennen et al, 1976).…”