Gas chromatographic determination of water in natural products by reaction with 2,2-dimethoxypropane

Mary, Nouri Y.

doi:10.1002/jps.2600580910

Cited by 7 publications

(1 citation statement)

References 22 publications

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“…1). DMP has been used for dehydration of small specimens prior to electron microscopy (Muller and Jacks, 1975;Maser and Trimble, 1977;Jones and Gwynn, 1991), light microscopy (Prento, 1978;Zuniga et al, 1994) and staining (Conway and Kiernan, 1998), and to determine the water contents of various materials by GC (Mary, 1969;Blanco et al, 1979;Dix et al, 1989). In addition, in the analysis of some compounds in biological samples by GC, DMP has been used as the sample preparation solution for analysis of diols (Porter and Auansakul, 1982;Smith, 1984;Rodman and Ross, 1986;Flanagan et al, 1987) and as a water scavenger, deproteinizing agent and a methylating agent for analysis of fatty acids (Mason and Waller, 1964;Shimasaki et al, 1977;Tserng et al, 1981;Fedrigo et al, 1999).…”

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confidence: 99%

Determination of dimethyl sulfoxide and dimethyl sulfone in urine by gas chromatography–mass spectrometry after preparation using 2,2‐dimethoxypropane

Takeuchi

Yamamoto

Narai

et al. 2009

Biomedical Chromatography

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A method for routinely determination of dimethyl sulfoxide (DMSO) and dimethyl sulfone (DMSO(2)) in human urine was developed using gas chromatography-mass spectrometry. The urine sample was treated with 2,2-dimethoxypropane (DMP) and hydrochloric acid for efficient removal of water, which causes degradation of the vacuum level in mass spectrometer and shortens the life-time of the column. Experimental DMP reaction parameters, such as hydrochloric acid concentration, DMP-urine ratio, reaction temperature and reaction time, were optimized for urine. Hexadeuterated DMSO was used as an internal standard. The recoveries of DMSO and DMSO(2) from urine were 97-104 and 98-116%, respectively. The calibration curves showed linearity in the range of 0.15-54.45 mg/L for DMSO and 0.19-50.10 mg/L for DMSO(2). The limits of detection of DMSO and DMSO(2) were 0.04 and 0.06 mg/L, respectively. The relative standard deviations of intra-day and inter-day were 0.2-3.4% for DMSO and 0.4-2.4% for DMSO(2). The proposed method may be useful for the biological monitoring of workers exposed to DMSO in their occupational environment.

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