Gastric microbiota and <i>Helicobacter pylori</i> in Indonesian population

Miftahussurur, Muhammad; Waskito, Langgeng Agung; El–Serag, Hashem B.; Ajami, Nadim J.; Nusi, Iswan Abbas; Syam, Ari Fahrial; Matsumoto, Takashi; Rezkitha, Yudith Annisa Ayu; Doohan, Dalla; Fauzia, Kartika Afrida; Maimunah, Ummi; Sugihartono, Titong; Uchida, Tomohisa; Yamaoka, Yoshio

doi:10.1111/hel.12695

Cited by 26 publications

(38 citation statements)

References 51 publications

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“…Previous studies have demonstrated that H. pylori infection is associated with marked changes in the gastric microbiota [14,19,[22][23][24]. Here, we confirmed that H. pylori-negative and -positive patients had different bacterial profiles characterized by less richness of gastric microbiota and the reduction in abundances of Blautia, Ralstonia, Faecalibacterium, Methylobacterium, and Megamonas in H. pylori-positive patients.…”

Section: Discussionsupporting

confidence: 87%

Long-term persistence of gastric dysbiosis after eradication of Helicobacter pylori in patients who underwent endoscopic submucosal dissection for early gastric cancer

et al. 2020

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Background Gastric microbiome, other than Helicobacter pylori, plays a role in the tumorigenesis of gastric cancer (GC). Patients who undergo endoscopic submucosal dissection for early GC have a high risk of developing metachronous GC even after successful eradication of H. pylori. Thus, we investigated the microbial profiles and associated changes in such patients after the eradication of H. pylori. Methods A total of 19 H. pylori-infected patients with early GC who were or to be treated by endoscopic resection, with paired biopsy samples at pre- and post-eradication therapy, were retrospectively enrolled. Ten H. pylori-negative patients were enrolled as controls. Biopsy samples were analyzed using 16S rRNA sequencing. Results H. pylori-positive patients exhibited low richness and evenness of bacteria with the deletion of several genera, including Blautia, Ralstonia, Faecalibacterium, Methylobacterium, and Megamonas. H. pylori eradication partially restored microbial diversity, as assessed during a median follow-up at 13 months after eradication therapy. However, post-eradication patients had less diversity than that in the controls and possessed a lower abundance of the five genera mentioned above. The eradication of H. pylori also altered the bacterial composition, but not to the same extent as that in controls. The microbial communities could be clustered into three separate groups: H. pylori-negative, pre-eradication, and post-eradication. Conclusion Changes in dysbiosis may persist long after the eradication of H. pylori in patients with a history of GC. Dysbiosis may be involved in the development of both primary and metachronous GC after the eradication of H. pylori in such patients.

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Section: Discussionsupporting

confidence: 87%

Long-term persistence of gastric dysbiosis after eradication of Helicobacter pylori in patients who underwent endoscopic submucosal dissection for early gastric cancer

et al. 2020

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“…Thus, the role of baseline demographic characteristics remained to be elucidated in future studies. The compositional analysis showed that H pylori frequently dominated the gastric mucosa of infected individuals while other phyla, including Actinobacteria , Bacteroidetes , Firmicutes , and Fusobacteria, tended to become relatively low 6,19,20,22‐25 . In contrast, gastric microbiota of noninfected samples were found to be enriched with Streptococcus 6,22,23 .…”

Section: Gastrointestinal Microbiota Reshaping Upon H Pylori Infectionmentioning

confidence: 96%

“…The phenomenon could be attributed to the harsh environment at the end stage since H pylori itself was sharply reduced through gastric carcinogenesis 26 . Most studies revealed that individuals infected with H pylori had distinct community structures compared with H pylori ‐negative counterparts 19‐23,25,27 . However, in two earlier studies, neither alpha diversity nor community structures were found to be different between H pylori ‐positive and H pylori‐ negative groups 4,28 .…”

Section: Gastrointestinal Microbiota Reshaping Upon H Pylori Infectionmentioning

confidence: 97%

“…Generally, researchers showed that colonization with H pylori was associated with significantly reduced alpha diversity (Figure 2; Table 1). 6,18‐25 Quantitatively, one study detected an inverse relationship between microbial diversity and Helicobacter abundance 18 . Some researchers explored this inverse association during the progression from gastritis to gastric neoplasms, reporting the correlation tended to be weak in cancer cases 19 .…”

Section: Gastrointestinal Microbiota Reshaping Upon H Pylori Infectionmentioning

confidence: 99%

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Helicobacter pylori infection and eradication: Exploring their impacts on the gastrointestinal microbiota

2020

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The rapid development of microbiota research has remolded our view of human physiological and pathological processes. Among all the gastrointestinal microorganisms, Helicobacter pylori (H pylori) is probably the most notorious constituent. Although half of the adults worldwide are infected with H pylori, their clinical manifestations vary widely, suggesting other microorganisms beyond H pylori may play a role in determining clinical outcomes. Recently, many studies have put effort into elucidating the crosstalk within the human microbiota, some of which specifically explored the interplay between H pylori and other gastrointestinal microbial members. In this work, we reviewed these potential interactions. Meanwhile, the impacts of H pylori eradication therapy on gastrointestinal microbial homeostasis were summarized in terms of diversity, composition, functional capacity, and antibiotic resistance.

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“…Gastric microbiota sequencing studies have reported Firmicutes, Proteobacteria, Actinobacteria, Bacteroidetes, and Fusobacteria phyla colonization [ 5 , 6 ]. This microbiota variability is multifactorial and associated with diet, drugs, antibiotics, immune response, chronic processes, and H. pylori infections [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

MALDI-TOF MS and 16S RNA Identification of Culturable Gastric Microbiota: Variability Associated with the Presence of Helicobacter pylori

et al. 2020

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Helicobacter pylori is the main bacteria associated with gastroduodenal diseases. Recent studies have reported that gastric microbiota might be modified by the H. pylori colonization, favoring gastric lesions′ development. In Chile, the region of La Araucanía concentrates a high risk of gastric cancer associated with Helicobacter pylori colonization, rurality, poverty, and Mapuche ethnicity. Hence, we aimed to identify the culturable gastric microbiota and characterize its variability at different stages of epithelial injury, based on its H. pylori colonization in dyspeptic patients from this Chilean region. Microaerophilic bacteria strains were isolated from antrum biopsies of 155 dyspeptic patients′ biopsies and identified using MALDI-TOF MS or 16sRNA gene sequencing for non-pylori species identification, and UreC gene amplification for H. pylori confirmation. We found 48 species from 18 families, mainly belonging to Neisseriaceae (21.3%), Streptococcaceae (20.0%), Actynomicetaceae (9.0%), Enterobacteriaceae, and Lactobacillaceae (4.5%); however, Streptococcaceae and Actinomycetaceae families showed a significant reduction in samples infected with H. pylori, along with a considerably lower diversity of species. Our results revealed a microbiota modification due to H. pylori colonization associated with the gastric epithelial state, suggesting a potential microbiota role for developing and progressing gastric diseases.

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Gastric microbiota and Helicobacter pylori in Indonesian population

Cited by 26 publications

References 51 publications

Long-term persistence of gastric dysbiosis after eradication of Helicobacter pylori in patients who underwent endoscopic submucosal dissection for early gastric cancer

Long-term persistence of gastric dysbiosis after eradication of Helicobacter pylori in patients who underwent endoscopic submucosal dissection for early gastric cancer

Helicobacter pylori infection and eradication: Exploring their impacts on the gastrointestinal microbiota

MALDI-TOF MS and 16S RNA Identification of Culturable Gastric Microbiota: Variability Associated with the Presence of Helicobacter pylori

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