Gastroprotective activity of methanol extract and marrubiin obtained from leaves of Marrubium vulgare L. (Lamiaceae)

Abstract

The results of this study show that the extract of M. vulgare and marrubiin displays antiulcer activity and that this effect can be partly attributed to the isolated diterpene.

Phytochemical Screening and Anti-Inflammatory Effect of Marrubium vulgare L. Methanol Extract on Carrageenan-Induced Paw Inflammation in Rats

“…3 Externally, they have been used in wounds and ulcers healing. 2 Nowadays, according to various studies, there is not any debate about the distinct effects of Marrubium genus such as cardioprotective effects, 4,5 anti-oedematogenic action, 6 gastroprotective role, 7 anti-hypertensive effect in diabetic rats, 8 analgesic effect in painful diabetic neuropathy, 9 and antispasmodic effects. 10 Also, it has been verified that administration of Marrubium species, including Marrubium vulgare, causes clear hypoglycemia, 11 reduces serum cholesterol and triglyceride and has a favorable effect on carbohydrate and lipid metabolism.…”

Marrubium vulgare L.: A review on phytochemical and pharmacological aspects

“…vulgare is used in traditional medicine for the treatment of gastrointestinal and respiratory disorders in Brazil. A diterpene and marrubiin have been isolated from methanol extract of M. vulgare leaves and evaluated for gastroprotective properties [80]. M. vulgare extract (50 and 100 mg/kg) produces a significant protective effect in ethanol-induced ulcers model in mice and effect was comparable with omeprazole (30 mg/kg).…”

“…Treated mice showed tachycardia 1 h after intake of the infusion and loss of appetite 3 h after intake of the infusion. In another experiment, five female rats were treated orally with a single dose of 2000 mg/kg extract of M. vulgare for an acute toxicity study [80]. After 14 days, no changes were detected in rat skin, fur, eyes, mucous membrane (nasal), central nervous system, and autonomic nervous system.…”

Chromatographic Fingerprint Analysis of Marrubiin in Marrubium vulgare L. via HPTLC Technique

“…16 Furthermore, in a recent study gastroprotective activity of the methanolic extract of M. vulgare had been revealed due to the presence of marrubiin as evidenced by inhibitory effect of both marrubiin and the methanol extract of M. vulgare on the indomethacin-induced ulcers. 17 Generally, considering loads of published data over the past century on the chemical and biological aspects of marrubiin in the genus Marrubium (Lamiaceae), we could consider this compound as a biomarker within the plants of this genus. In view of these facts, High Performance Thin Layer Chromatography (HPTLC) has been widely used as a rapid, precise and cost-effective method for determination of biological compounds from medicinal plants; accordingly, in this study we adopted HPTLC as a promising technique for determination of marrubiin quantitatively within the M. vulgare extract.…”