GC‐MS‐Based Metabolite Profiling of <i>Cosmos caudatus</i> Leaves Possessing Alpha‐Glucosidase Inhibitory Activity

Javadi, Neda; Abas, Faridah; Hamid, Azizah Abdul; Simoh, Sanimah; Shaari, Khozirah; Ismail, Intan Safinar; Mediani, Ahmed; Khatib, Alfi

doi:10.1111/1750-3841.12491

Cited by 67 publications

(97 citation statements)

References 27 publications

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“…This finding might be due to the ability to dissolve metabolites that contribute to α-glucosidase inhibition activity. This result was in contrast with a previous study, which reported that absolute ethanol and methanol had the highest bioactivity compared to aqueous solvents [18]. The α-glucosidase inhibition activity are consistent with the TPC and DPPH results, wherein FD exhibited the highest TPC and antioxidant activity, and OD showed the lowest values for both.…”

Section: Effect Of Drying Methods and Solvent On α-Glucosidasesupporting

confidence: 55%

“…However, the absolute solvent exhibited lower activity compared to 80 % solvent extracts. According to previous studies, adding water to the solvent at a low quantity enhances its antioxidant activity [3,18]. In light of these findings, P. niruri could be used in medicinal applications with a high therapeutic value.…”

Section: Effect Of Drying Methods and Solvent On The Antioxidantmentioning

confidence: 87%

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Relationship Between Metabolites Composition and Biological Activities of Phyllanthus niruri Extracts Prepared by Different Drying Methods and Solvents Extraction

Mediani

Abas

Khatib

et al. 2015

Plant Foods Hum Nutr

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The study investigated the changes in the metabolite, antioxidant and α-glucosidase inhibitory activities of Phyllanthus niruri after three drying treatments: air, freeze and oven dryings. Water extracts and extracts obtained using different solvent ratios of ethanol and methanol (50, 70, 80 and 100%) were compared. The relationships among the antioxidant, α-glucosidase inhibitory activity and metabolite levels of the extracts were evaluated using partial least-square analysis (PLS). The solvent selectivity was assessed based on the phytochemical constituents present in the extract and their concentrations quantitatively analyzed using high performance liquid chromatography. The freeze-dried P. niruri samples that were extracted with the mixture of ethanol or methanol with low ratio of water showed higher biological activity values compared with the other extracts. The PLS results for the ethanolic with different ratio and water extracts demonstrated that phenolic acids (chlorogenic acid and ellagic acid) and flavonoids were highly linked to strong α-glucosidase inhibitory and antioxidant activities.

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Section: Effect Of Drying Methods and Solvent On α-Glucosidasesupporting

confidence: 55%

Section: Effect Of Drying Methods and Solvent On The Antioxidantmentioning

confidence: 87%

Relationship Between Metabolites Composition and Biological Activities of Phyllanthus niruri Extracts Prepared by Different Drying Methods and Solvents Extraction

Mediani

Abas

Khatib

et al. 2015

Plant Foods Hum Nutr

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“…Javadi et al . () reported that α‐glucopyranoside, succinic acid, octadecanoic acid, α‐linolenic acid, D‐fructose, propenoic acid and valine from Cosmos caudatus leaves were effective at accelerating α‐glucosidase inhibitory activity. This result is also consistent with our findings given the abundant presence of all of these compounds in P. macrocarpa plant extracts.…”

Section: Resultsmentioning

confidence: 99%

Screening of Various Parts of P haleria macrocarpa Plant for α-Glucosidase Inhibitory Activity

Sabina

Zaidul

Ghafoor

et al. 2015

Journal of Food Biochemistry

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Phaleria macrocarpa is an herbal plant used in Malaysia to enhance vitality. The aim of this study was to screen the α-glucosidase inhibitory activity of different parts (fruit flesh, leaves and stem) of P. macrocarpa. Methanol (polar) and n-hexane (nonpolar) extracts, obtained by room temperature solvent extraction, were evaluated for in vitro α-glucosidase activity inhibition. The compounds were identified by using gas chromatography-mass spectrometry (GC-MS) according to their similarity index of >70%, which might be responsible for α-glucosidase inhibitory activity. The methanol extract of the fruit flesh had the highest yield (25.6 ± 0.5%), whereas the n-hexane extract of the stem is more effective against α-glucosidase activity (IC50 0.8 ± 0.1 μg/mL). The fruit flesh (IC501.3 ± 0.2 μg/mL) and leaves (IC501.6 ± 0.6 μg/mL) had also well effectively. The identified metabolites are predominantly phenolics, carbohydrates, triterpenes and organic acids, such as D-fructose, squalene, α-linolenic acid and α-D-glucopyranoside. In-depth chemical profiling using GC-MS was performed for the first time for this plant to assess the likely compounds present in the extract that could be associated with anti-hyperglycemic activity. Of the three parts tested, every part indicates the potential α-glucosidase inhibitory activity and hexane extract of stem showed more inhibitory activity among all extracts. Thus, P. macrocarpa can attenuate hyperglycemia by potently inhibiting carbohydrate hydrolyzing enzymes, making it a viable plant as a source of natural compounds for the management of type 2 diabetes mellitus. PRACTICAL APPLICATIONP. macrocarpa (Scheff.) Boerl. belongs to the Thymelaeaceae family, which is known as "Mahkota Dewa" is one of the well-known traditional herbs in South Asian countries. Every part of this plant has been reported to be used as a traditional medicine for diabetic treatment for many years. In the present study, the ability of this plant to inhibit carbohydrate hydrolyzing enzymes (α-glucosidase) was explored. All extracts tested exhibited the content to inhibit yeast α-glucosidase enzymes in vitro, and these beneficial effects appear to be due to some bioactive compounds in P. macrocarpa. Our preliminary investigation gives a principle for further animal and clinical studies of a possible use of P. macrocarpa for the management of diabetes mellitus.

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“…The percentage of yield of the ethanol extract was found to be 10%, which is the lowest yield. This result indicates that the extraction yield depends on the solvent polarity where the more polar solvent gives the higher yield (Javadi et al, ). The results demonstrated that water was the most suitable extraction solvent according to the extract's yield.…”

Section: Resultsmentioning

confidence: 97%

“…The extracts were prepared according to the method of Javadi et al () with some modifications. The extractions were performed by using two solvents, ethanol, and water, at various ratios (100% ethanol, 80% ethanol, 60% ethanol, 40% ethanol, 20% ethanol, and 0% ethanol) with six replicates.…”

Section: Methodsmentioning

confidence: 99%

Extraction of α-glucosidase inhibitory compounds fromPhaleria macrocarpafruit flesh using solvent, sonication, and subcritical carbon dioxide soxhlet methods

et al. 2017

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The objective of this study was to compare α‐glucosidase inhibitory activity of Phaleria macrocarpa extracts using subcritical carbon dioxide (subCO2) soxhlet extraction method with sonication and solvent extraction methods. The extraction was performed by subCO2 soxlet using carbon dioxide and the conventional organic solvent‐based sonication using ethanol and water, solvent extraction using methanol, and n‐hexane solvents. The yields and α‐glucosidase inhibitory activity of the P. macrocarpa extracts were analyzed. The yields using sonication, solvent extraction, and subCO2 soxhlet extraction were approximately 10.2 ± 1.0 to 25.4 ± 0.6% and 0.9 ± 0.1 to 2.0 ± 1.0%. The IC50 value of subCO2 soxhlet, sonication, and solvent extraction methods were 4.35, 7.34, and 1.9 µg/mL, respectively. The α‐glucosidase inhibitory activity of P. macrocarpa extract using subCO2 soxhlet method was higher than the conventional sonication method and lower than the solvent extraction method. Overall, subCO2 soxhlet extraction of P. macrocarpa represents a promising method to produce organic solvent free and highly α‐glucosidase inhibitory compounds containing extracts. Practical applications This study compared the conventional and nonconventional extraction methods for separating bioactive compounds from P. macrocarpa. Plant extracts contain a large variety of bioactive compounds with many other constituents including pharmaceuticals that require separations, purifications, and fractionations for further processing. Pharmaceutical industries are always looking for green processing methods obtaining green products. Nonconventional methods are of great interest owing to their efficiency such as fast, organic‐residues‐free, low temperature processing, and cost‐effective compare to any conventional method. Moreover, nonconventional methods are technologically advanced and claimed to be green with innumerable applications. Bioactive compounds of P. macrocarpa extracted by nonconventional methods may retain the natural quality that is of organic residues free and can be further exploited for the pharmaceutical, cosmetic, functional foods, and neutraceuticals industries. The environmental issues and health concerns of society are also demanding the green technology in our daily life.

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GC‐MS‐Based Metabolite Profiling of Cosmos caudatus Leaves Possessing Alpha‐Glucosidase Inhibitory Activity

Cited by 67 publications

References 27 publications

Relationship Between Metabolites Composition and Biological Activities of Phyllanthus niruri Extracts Prepared by Different Drying Methods and Solvents Extraction

Relationship Between Metabolites Composition and Biological Activities of Phyllanthus niruri Extracts Prepared by Different Drying Methods and Solvents Extraction

Screening of Various Parts of P haleria macrocarpa Plant for α-Glucosidase Inhibitory Activity

Extraction of α-glucosidase inhibitory compounds fromPhaleria macrocarpafruit flesh using solvent, sonication, and subcritical carbon dioxide soxhlet methods

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