GDF9 gene polymorphism and its association with litter size in two Russian sheep breeds

Gorlov, I.F.; Kolosov, Y. A.; Широкова, Н.В.; Getmantseva, Lyubov; Slozhenkina, M I; Mosolova, N I; Bakoev, Nekruz; Леонова, М. А.; Kolosov, Anatoly; Zlobina, E.Yu.

doi:10.1007/s12210-017-0659-2

Cited by 26 publications

(14 citation statements)

References 25 publications

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“…This observation is in agreement with frequencies observed in Belclare and Cambridge breeds [22], Arkha Merino sheep [36], Moghani and Ghezed breeds [33], Sangsari sheep [37], Baluchi sheep [23], Hisar sheep [38], Moghani sheep [39], and Saidi sheep [40]. On the other hand, Gorlov et al [41] reported that the frequency of the mutant allele was high and no wild-type genotypes (AA) were detected in Volgograd and Salsk sheep, as was the case in Lori sheep [42,43]. Whereas Elfiky et al [40] reported that all of the observed genotypes were wild type in Ossimi sheep.…”

Section: Source Of Variation Df Sm Ms F-ratio Pr>fsupporting

confidence: 90%

G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep

et al. 2021

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Background and Aim: Sudanese desert sheep encompass different sheep breeds named according to the different Sudanese tribes that rear them such as the Dubasi, Shugor, and Watish sheep. The objectives of this study were to screen for G1 point mutation in the polymorphic growth differentiation factor 9 (GDF9) gene, investigate its association with litter size, and construct the phylogeny of the different tribal breeds that belong to the Sudanese Desert sheep tribal types. Materials and Methods: Genomic DNA was extracted from whole blood of three tribal Desert sheep breeds (Dubasi, Watish, and Shugor) using the guanidine chloride method. Polymerase chain reaction-restriction fragment length polymorphism with HhaI restriction enzyme and sequencing techniques was used for genotyping the GDF9 locus for possible mutations associated with litter size in the three desert sheep tribal types. Results: G1 mutation in GDF9 caused the replacement of Arginine by Histidine at residue 87. The wild type allele (A) had the highest frequency, whereas the mutant type allele (a) had the lowest in all the sequenced subtypes. The genotype frequencies of the wild type ewes (AA) were higher than the heterozygous (Aa) and the mutant type (aa) frequencies in the three studied desert sheep types. No significant differences were found in the allele frequency between the three tribal types. Litter size was significantly influenced by the genotypes of GDF9 gene, parities, and subtypes (p≤0.01, 0.01, and 0.05, respectively). In the Watish sheep type, heterozygous sheep in their second parity recorded the highest litter size. Sequence alignment of GDF9 gene samples with the database entry indicated that all three tribal types were similar and identical to the reference sequence. The phylogenetic tree revealed that Shugor is the common ancestor of the studied types and Watish is more closely related to Shugor than Dubasi. This result mi ght partly explain the lower reproductive performance of Dubasi compared to Watish and Shugor. Conclusion: The presence of one copy of GDF9 gene increased litter size in the studied Sudanese Desert sheep. This locus may be used as a biomarker for litter size improvement through genotypic selection and allele or gene introgression.

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Section: Source Of Variation Df Sm Ms F-ratio Pr>fsupporting

confidence: 90%

G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep

et al. 2021

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“…The FecG 1 mutation found by Hanrahan et al (2004) proved to a have a phenotypic effect in two Chilean breeds, Chilota and Aracuana (Paz et al, 2014); another one from a Hanrahan study FecG 7, was present in Norwegian White sheep (Våge, et al, 2013). There is also an indication that there is an association of the GDF9 polymorphism (GDF9/G1 and GDF9/G4 sites) and litter size in Slask and Volgograd sheep breeds in Russia (Gorlov et al, 2017).…”

Section: The Best Known Booroola Mutation (Fecb B ) Responsible For Hmentioning

confidence: 89%

Frequency of BMP15 and GDF9 mutations increasing litter size and their phenotypic effects in Olkuska sheep population

Drobik-Czwarno

Martyniuk

Nowak-Życzyńska

et al. 2021

Annals of Animal Science

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Two mutations affecting the ovulation rate and litter size are segregating in Olkuska sheep population, FecXO in the BMP15 gene, and the G7 site mutation in GDF9 gene. Homozygous carriers of both mutations are hyperprolific, contrary to the sterility observed in homozygous carriers of most other BMP15 and GDF9 mutations. The objective of this study was to assess frequency and phenotypic effects of both mutations. Blood samples were obtained from 740 individuals, 111 rams and 629 ewes, out of which 91 rams and 561 ewes were successfully genotyped for the BMP15 and GDF9 loci. The reproductive performance included a number of lambs born/born alive and a number of lambs reared until 60 days of age, and for a subset of ewe ovulation rates. The study proved a high frequency of the FecXO mutation in two flocks that have been selected for many years for increased litter size (0.7 - 1.0 in breeding ewes and rams respectively), and a moderate frequency in another 19 private flocks (0.4 - 0.5). The frequency of the GDF9 mutation was low, with only 50 sheep out of 312 genotyped being carriers of the GDF9/G7 mutation, including three homozygous carriers. The FecXO mutation in the BMP15 had a significant effect on both litter size and the ovulation rate. The single copy in heterozygous carriers increased litter size by 0.255 (0.063), while the effect of two copies in homozygous genotypes was + 0.874 (0.081) lambs born. Due to the low frequency of the GDF9 mutation, it can only be preliminary concluded that litter size has been increased in double carries, both of the BM15 and GDF9 mutation, which may suggest their additive interaction. The positive effect of both mutations supports their direct use in selection programmes.

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“…Genotype and allele frequency, PIC , HE and NE were calculated. Then, the distribution of genotypes for each SNP in the studied population were tested for deviation from Hardy–Weinberg equilibrium by the Hardy–Weinberg law [ 30 , 39 ]. Statistical analyses were performed using SAS (V. 8.1) (SAS Institute Inc., Cary, NC, USA).…”

Section: Methodsmentioning

confidence: 99%

“…All those heterozygotes had the higher fertility than wild type but homozygotes were sterile except FecX Gr and FecX O [ 24 , 25 , 26 , 27 , 28 , 29 ]. GDF9 have been reported to be associated with litter size in Araucana creole sheep [ 1 ], Salsk and Volgograd sheep [ 30 ]. Like most mutations of BMP15 , the heterozygotes of GDF9 had the highest fertility.…”

Section: Introductionmentioning

confidence: 99%

Single Nucleotide Polymorphisms in the HIRA Gene Affect Litter Size in Small Tail Han Sheep

Pan

Cao

Guo

et al. 2018

Animals

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Simple SummaryLitter size is one of the most important reproductive traits in sheep. Two single nucleotide polymorphisms (SNPs), g.71874104G>A and g.71833755T>C, in the Histone Cell Cycle Regulator (HIRA) gene, were identified by whole-genome sequencing (WGS) and may be correlated with litter size in sheep. The two SNPs were genotyped and expression patterns of HIRA was determined in sheep breeds with different fecundity and in groups of Small Tail Han sheep producing large or small litters. Association analysis indicated that both SNPs were significantly correlated with litter size in Small Tail Han sheep. Furthermore, high levels of HIRA expression may have a negative effect on litter size in Small Tail Han sheep.AbstractMaintenance of appropriate levels of fecundity is critical for efficient sheep production. Opportunities to increase sheep litter size include identifying single gene mutations with major effects on ovulation rate and litter size. Whole-genome sequencing (WGS) data of 89 Chinese domestic sheep from nine different geographical locations and ten Australian sheep were analyzed to detect new polymorphisms affecting litter size. Comparative genomic analysis of sheep with contrasting litter size detected a novel set of candidate genes. Two SNPs, g.71874104G>A and g.71833755T>C, were genotyped in 760 Small Tail Han sheep and analyzed for association with litter size. The two SNPs were significantly associated with litter size, being in strong linkage disequilibrium in the region 71.80–71.87 Mb. This haplotype block contains one gene that may affect litter size, Histone Cell Cycle Regulator (HIRA). HIRA mRNA levels in sheep with different lambing ability were significantly higher in ovaries of Small Tail Han sheep (high fecundity) than in Sunite sheep (low fecundity). Moreover, the expression levels of HIRA in eight tissues of uniparous Small Tail Han sheep were significantly higher than in multiparous Small Tail Han sheep (p < 0.05). HIRA SNPs significantly affect litter size in sheep and are useful as genetic markers for litter size.

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GDF9 gene polymorphism and its association with litter size in two Russian sheep breeds

Cited by 26 publications

References 25 publications

G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep

G1 point mutation in growth differentiation factor 9 gene affects litter size in Sudanese desert sheep

Frequency of BMP15 and GDF9 mutations increasing litter size and their phenotypic effects in Olkuska sheep population

Single Nucleotide Polymorphisms in the HIRA Gene Affect Litter Size in Small Tail Han Sheep

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