Gene expression profiling in streptozotocin-induced diabetic rat liver in response to fungal polysaccharide treatment

Abstract

We established the gene expression profiling in streptozotocin (STZ)-induced diabetic rat liver in response to hypoglycemic fungal polysaccharides (EPS), using oligonucleotide microarray analysis. Differentially regulated genes showing higher fold change than 2 were identified and categorized through hierarchical clustering analysis. Among the 835 genes analyzed, 244 were up-regulated, while 321 were down-regulated after diabetes induction. Interestingly, many gene expressions altered after STZ-treatment mimicked a normal rat liver by EPS therapy. Most of these genes included genes involving cell structure and motility, immunity and defense, lipid, fatty acid and steroid metabolism, protein metabolism and modification, and signal transduction. More importantly, we found a total of 36 genes that showed significant fold changes in their expression that have not previously been examined in the context of diabetes. To validate the microarray results, we further confirmed the gene expression patterns by RT-PCR using four genes of interest (carboxylesterase 2, stearoyl-coenzyme A desaturase 1, insulin-like growth factor 1, and insulin-like growth factor binding protein 2). Taken together, EPS acted as a potent regulator of gene expression for a wide variety of genes in diabetic rat liver.

Hypoglyclemic Effects in Response to Abelmoshus Esculentus Treatment: A Research Framework using STZ-Induced Diabetic Rats

“…More than a half of gene analyzed (565 from total 835 genes) showing two-fold differences compared to the control. The differentially expressed genes involved in carbohydrate, amino acid metabolism, immunity and defense, lipid, fatty acid , steroid metabolism and signal transduction [50].…”

Curcumin regulates gene expression of insulin like growth factor, B-cell CLL/lymphoma 2 and antioxidant enzymes in streptozotocin induced diabetic rats

“…The Primers of GPX, CAT and SOD [28] as well as IGF-1 and β-actin genes [29] and Bcl-2 [30] were taken from literature. The primers were checked for their Tm values, hairpin loops, dimers, cross- dimers and number of repeats and runs using Net Primer (Oligoanalyzer 3.1).…”