Gene expression responses in human lung fibroblasts exposed to alpha particle radiation

GDF-15 gene expression alterations in human lymphoblastoid cells and peripheral blood lymphocytes following exposure to ionizing radiation

“…GDF-15 is a member of the tumor growth factor-β superfamily and a downstream target of p53 (42), and serves important roles in human diseases, such as cardiomyopathy (43). Previous studies have suggested that the GDF-15 gene may participate in γ-ray-induced DNA damage, as microarray analysis identified GDF-15 as a radiation-responsive gene (19,34,36,39). …”

“…It has previously been reported that alterations in GDF-15 gene expression in human pulmonary epithelial cells were dose- and time-dependent, following exposure to α-particle radiation (19). In accordance with a previous study (34), the present results demonstrated that GDF-15 expression was altered by γ-rays and neutron radiation.…”

“…Gene expression studies in human primary fibroblasts, keratinocytes (36–38) and pulmonary epithelial cells (19) suggested that growth/differentiation factor 15 ( GDF-15 ), which is also a target gene of p53, is a radiation-responsive gene. Radiation-induced alterations in GDF-15 expression are correlated with the time and dose of radiation exposure (19,34). Previous microarray analysis in our laboratory, demonstrated that GDF-15 , among other genes, was consistently upregulated in AHH-1 human lymphoblastoid cells following exposure to 0.5, 3 and 8 Gy of 60 Co γ-rays (39).…”

Key mechanisms involved in ionizing radiation-induced systemic effects. A current review

“…150 In a later study, a dose-dependent increase in GDF-15 gene expression was shown between 0.1 and 2 Gy. 172 This observation has been lately further strengthened by Chauhan et al, 173 who also showed that GDF-15 was a radiation-responsive gene in human lung fibroblasts after α-irradiation, which as a high-LET radiation is expected to induce primarily clustered DNA damage. 174 Sándor et al showed that GDF-15 was responsible for fibroblast radioresistance, since silencing the gene rendered the cells more radiation-sensitive.…”

Label-free electrochemical immunoassay of Bcl-2 protein expression on tumor cells

“…The classical techniques for analysis on gene and protein expression on cells in molecular biology and cytobiology research include enzyme-linked immunoassay [14], western blotting [15], immunohistochemistry [16], flow cytometry [17], and so on. Although these methods are valid and sensitive, most of them require special equipment, talented operator and the use of labels including fluorescein and isotope.…”