Generation of epidermal growth factor–expressing Lactococcus lactis and its enhancement on intestinal development and growth of early-weaned mice

Cheung, Queenie; Yuan, Zongfei; Dyce, Paul W.; Wu, De; DeLange, Kees; Li, Julang

doi:10.3945/ajcn.2008.27073

Cited by 59 publications

(58 citation statements)

References 46 publications

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“…Herein, we demonstrate that an intervention to reduce the CVA does not improve body weight; therefore, the intestinal changes may indicate the existence of altered epithelial cell proliferation in the CF condition or may reflect a tissue response to bacteria (29), which are overgrown in the CF intestine (30,31). rhIGFBP-3 treatment, as it decreased CVA length and muscle thickness, may be beneficial in ameliorating the intestinal propensity to develop mucous plugs, given the increased numbers of goblet cells in the distended villus and the decreased motility in the CF intestine (32).…”

Section: Discussionmentioning

confidence: 84%

IGF Binding Protein-3 Treatment Alters Intestinal Cell Proliferation But Not Body Weight of Adult Cystic Fibrosis Transmembrane Conductance Regulator Deficient Mice

Canale-Zambrano

Haston

2011

Pediatr Res

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ABSTRACT:The intestinal phenotype of cystic fibrosis (CF) transmembrane conductance regulator deficient mice includes altered cell homeostasis and a distended crypt-villus axis, which, in previous work, was inversely proportional to body weight. To investigate this correlation, herein, we treated CF mice with IGF binding protein-3 (IGFBP-3), a protein which, as it has potent effects on cell proliferation and apoptosis, we hypothesized would alter the intestinal cell homeostasis, and assessed body weight. Six-week-old C57BL/ 6JxBALB F2 CF and WT mice received recombinant human IGFBP-3 (rhIGFBP-3, 20 mg/kg) or vehicle treatment, and weight gain, serum protein levels, and intestinal histology were assessed. Administration of rhIGFBP-3 to CF mice significantly increased the number of Igfbp-3 positive cells in the intestine and partially reversed the hyperproliferative phenotype of intestinal crypts and muscularis externa, while not affecting apoptosis. Serum Igfbp-3 levels were increased, and Igf-I, albumin, and triglycerides measures were decreased in CF compared with WT mice. rhIGFBP-3 treatment significantly increased serum albumin and triglycerides but did not affect weight gain in CF mice. We have identified rhIGFBP-3 treatment to reduce intestinal cell proliferation, resulting in decreases in crypt depth and muscularis externa thickness in CF mice.

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Section: Discussionmentioning

confidence: 84%

IGF Binding Protein-3 Treatment Alters Intestinal Cell Proliferation But Not Body Weight of Adult Cystic Fibrosis Transmembrane Conductance Regulator Deficient Mice

Canale-Zambrano

Haston

2011

Pediatr Res

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“…Porcine EGF expressing L. lactis was generated via a recombinant approach as previously described (Cheung et al, 2009). Briefly, the mature EGF sequence was amplified from porcine RNA then ligated into an expression vector which was transformed into L. lactis.…”

Section: Production Of Recombinant Egf Using L Lactismentioning

confidence: 99%

“…We have previously generated a strain of porcine EGF producing Lactococcus lactis (EGF-LL) (Cheung et al, 2009), and demonstrated that supplementation of early-weaned piglets with this recombinant EGF increases body weight gain and gain to feed ratio (Bedford et al, 2014). The current study sought to further understand the in vivo mechanisms behind the improved growth and intestinal development observed with EGF containing culture supernatant through the investigation of the dynamic changes of potential key genes involved, jejunal enzyme activity, as well as histomorphological changes.…”

Section: Introductionmentioning

confidence: 99%

Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved

Bedford

Chen

Huynh

et al. 2015

Journal of Biotechnology

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“…Epidermal growth factor is an important factor which associates with the proliferation and differentiation of enterocyte, hence the stimulation of EGF introduces to villous growth [2]. Platelet-derived growth factor A is also an important factor which associates with the formation of villous structure [6].…”

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confidence: 99%

A Mouse Model Study for the Villous Atrophy of the Early Weaning Piglets

et al. 2010

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ABSTRACT. Early weaning induces villous atrophy in the small intestine of piglets. We evaluated an influence of early weaning at 16 days old in mice for the use of villous atrophy model observed in early-weaned piglets. Five pregnant BALB/c mice were obtained and half of pups were weaned at 16 days old (early-weaned), while the others were allowed to suckle. Their small intestine was collected at 17, 18 and 19 days old in each group. Villous was shorting at 17 and 18 days old, but obscured at 19 days old. The gene expressions of epidermal and platelet-derived growth factor were associated with the villous height. Early weaning induced villous atrophy in the mouse small intestine as well as the piglets.KEY WORDS: early weaning, epidermal growth factor, mouse model, platelet-derived growth factor, villous atrophy.

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Generation of epidermal growth factor–expressing Lactococcus lactis and its enhancement on intestinal development and growth of early-weaned mice

Abstract: This study showed that EGF-LL had beneficial effects on the intestinal growth of newly weaned mice. The combination of growth factor delivery and a probiotic approach may offer possibilities for formulating dietary supplements for children during their weaning transition stage.

Cited by 59 publications

References 46 publications

IGF Binding Protein-3 Treatment Alters Intestinal Cell Proliferation But Not Body Weight of Adult Cystic Fibrosis Transmembrane Conductance Regulator Deficient Mice

IGF Binding Protein-3 Treatment Alters Intestinal Cell Proliferation But Not Body Weight of Adult Cystic Fibrosis Transmembrane Conductance Regulator Deficient Mice

Epidermal growth factor containing culture supernatant enhances intestine development of early-weaned pigs in vivo: Potential mechanisms involved

A Mouse Model Study for the Villous Atrophy of the Early Weaning Piglets

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