Generation of Expressed Sequence Tags of Random Root cDNA Clones of Brassica napus by Single-Run Partial Sequencing

Park, Yu Shin; Kwak, June M.; Kwon, O‐Yu; Kim, Yong‐Sung; Lee, Dae Sil; Cho, Moo Je; Lee, Hyung Hoan; Nam, Hong Gil

doi:10.1104/pp.103.2.359

Cited by 77 publications

(46 citation statements)

References 36 publications

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“…The PSBA gene clone was isolated from a cDNA library of Brassica rapa (Kwak et al, 1997). The 18S rRNA probe was isolated from Brassica napus (Park et al, 1993).…”

Section: Rna Gel-blot Analysismentioning

confidence: 99%

Photomorphogenic development of the Arabidopsisshy2–1D mutation and its interaction with phytochromes in darkness

et al. 1998

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SummaryWe previously reported a photomorphogenic mutation of Arabidopsis thaliana, shy2-1D, as a dominant suppressor of a hy2 mutation. Here, we report that shy2-1D confers various photo-responsive phenotypes in darkness and the dark phenotypes of the mutant are affected by phytochrome deficiency. Dark-grown seedlings of the mutant developed several photomorphogenic characteristics such as short hypocotyls, cotyledon expansion and opening, and partial differentiation of plastids. When grown further in darkness, the mutant plant underwent most of the developmental stages of a light-grown wild-type plant, including development of foliar leaves, an inflorescence stem with cauline leaves, and floral organs. In addition, two light-inducible genes, the nuclear-encoded CAB and the plastid-encoded PSBA genes, were highly expressed in the dark-grown mutant seedlings. Furthermore, reduced gravitropism, a phytochrome-modulated response, was observed in the mutant hypocotyl in darkness. Thus, shy2-1D is one of the most pleiotropic photomorphogenic mutations identified so far. The results indicate that SHY2 may be a key component regulating photomorphogenesis in Arabidopsis. Surprisingly, double mutants of the shy2-1D mutant with the phytochrome-deficient mutants hy2, hy3 (phyB-1) and fre1-1 (phyA-201) showed reduced photomorphogenic response in darkness with a longer hypocotyl, a longer inflorescence stem, and a lower level expression of the CAB gene than the shy2-1D single mutant. These results showed that phytochromes function in darkness in the shy2-1D mutant background. The implications of these results are discussed.

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“…The PSBA gene clone was isolated from a cDNA library of Brassica rapa (Kwak et al, 1997). The 18S rRNA probe was isolated from Brassica napus (Park et al, 1993).…”

Section: Rna Gel-blot Analysismentioning

confidence: 99%

Photomorphogenic development of the Arabidopsisshy2–1D mutation and its interaction with phytochromes in darkness

et al. 1998

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“…Chinese cabbage belongs to the genus Brassica, which comprises many economically important vegetable plants, especially in Korea, China, and Japan. In addition, Brassica has served as a favorite model system for various biological processes in plants (Park et al, 1993). The relatively small genome size of Chinese cabbage (approximately 7.7 x 10sbp per haploid genome), only a few times larger than that of Arabidopsis, greatly simplifies both genetic and molecular analyses of genes (Croy et al, 1993).…”

mentioning

confidence: 99%

“…Various other organisms, such as nematode (McCombie et al, 1992; * Corresponding author; e-mail mjcho@nongae.gsr,w.ac.kr; fax 82-591-759-9363. Waterston et al, 1992), mouse (Hoog, 1991), and severa1 plants (Uchimiya et al, 1992;Hofte et al, 1993;Keith et al, 1993;Park et al, 1993;Newman et al, 1994;Sasaki et al, 1994) have also been examined by extensive sequencing of randomly selected cDNA clones. The enormous accumulation of ESTs has thus led to the establishment of dbEST (Boguski et al, 1993(Boguski et al, , 1994.…”

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confidence: 99%

Expressed Sequence Tags of Chinese Cabbage Flower Bud cDNA

Lim

Kim

et al. 1996

Plant Physiology

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We randomly selected and partially sequenced cDNA clones from a library of Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower bud cDNAs. Out of 1216 expressed sequence tags (ESTs), 904 cDNA clones were unique or nonredundant. Five hundred eighty-eight clones (48.4%) had sequence homology to functionally defined genes at the peptide level. Only 5 clones encoded known flower-specific proteins. Among the cDNAs with no similarity to known protein sequences (628), 184 clones had significant similarity to nucleotide sequences registered in the databases. Among these 184 clones, 142 exhibited similarities at the nucleotide level only with plant ESTs. Also, sequence similarities were evident between these 142 ESTs and their matching ESTs when compared using the deduced amino acid sequences. Therefore, it is possible that the anonymous ESTs encode plant-specific ubiquitous proteins. Our extensive EST analysis of genes expressed in floral organs not only contributes to the understanding of the dynamics of genome expression patterns in floral organs but also adds data to the repertoire of all genomic genes.

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“…Recently, a large number of cDNA clones were identified by random sequencing from plants including maize (Keith et al, 1993), Arabidopsis thaliana (Hofte et al, 1993;Newman et al, 1994), Brassica napes (Park et al, 1993) and rice (Uchimiya et al,1992;Sasaki et al,1994). Our objective is to obtain information for genes expressed at meiosis in higher plants.…”

Section: Introductionmentioning

confidence: 99%

Large-scale Sequencing of Meiosis-associated Genes from a cDNA Library of Lily Microsporocytes.

Morohashi

Takase

Hotta

et al. 2000

Plant Biotechnology

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Lily, Lilium longiflorum has been used to study meiosis for advantages of the synchronous development among sporogenous cells within an anther. In order to identify genes expressed at meiosis, we carried out random sequencing of a cDNA library which represented the mRNAs from microsporocytes of L. longiflorum at the zygotene stage of meiotic prophase. To avoid redundancy of eDNA clones and to obtain novel sequence information efficiently, weexploited a simple and versatile "self hybridization" strategy . Single -run DNA sequence data were obtained from 418 cDNA clones.Deduced amino acid sequences of 171 cDNA clones, which comprised 41% of the selected cDNA clones, showed significant similarity to amino acid sequences registered in the database. These included eDNA sequences for previously identified meiosis-associated genes. However, 59% of the selected clones showed no significant homology to known gene products.

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Generation of Expressed Sequence Tags of Random Root cDNA Clones of Brassica napus by Single-Run Partial Sequencing

Cited by 77 publications

References 36 publications

Photomorphogenic development of the Arabidopsisshy2–1D mutation and its interaction with phytochromes in darkness

Photomorphogenic development of the Arabidopsisshy2–1D mutation and its interaction with phytochromes in darkness

Expressed Sequence Tags of Chinese Cabbage Flower Bud cDNA

Large-scale Sequencing of Meiosis-associated Genes from a cDNA Library of Lily Microsporocytes.

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