Genetic diversity of chickpea (Cicer arietinum L.) germplasm in Pakistan as revealed by RAPD analysis

Ahmad, Faraz; Khan, Azeem Iqbal; Awan, Faisal Saeed; Sadia, Bushra; Sadaqat, Hafeez Ahmad; Bahadur, Sultan

doi:10.4238/vol9-3gmr862

Cited by 29 publications

(24 citation statements)

References 13 publications

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“…Although, great efforts to enhance the production of forage and grain legumes through the conventional breeding approaches have been made (Ahmad et al, 2010). However, due to existence of the wide genotype × environment interactions, which impacts the grain yield and the related traits, these genetic improvements approach of conventional breeding are slow (Kumar and Ali, 2006).…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity and Field Performance of Mung Bean, Faba Bean and Lentil Genotypes in the Kingdom of Saudi Arabia

Alghamdi

Al-Faifi

Migdadi

et al. 2017

IJAB

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Grain legumes are grown for their high proteins and biological nitrogen fixation ability. In this study, 3 mung bean, 4 lentil and 20 faba bean genotypes from the Kingdom of the Saudi Arabia were evaluated for genetic diversity. There were significant differences in the morphological and molecular characteristics in the tested mung bean (Vigna radiata L.), lentil (Lens culinaris Medik.) and faba bean (Vicia faba L.) genotypes. The sequence-related amplified polymorphism (SRAP) and amplified fragments length polymorphism (AFLP) markers exhibited a considerable genetic diversity among the tested genotypes of all three legume crops. The genotypes differed for plant height, primary branches, pod length, pods per plant, grain per pod and 100-grain weight. All the tested genotypes of mung bean, lentil and faba bean differed in the number of alleles. Maximum number of alleles per primer combination was 112 in mung bean, 72 in lentil and 126 in faba bean.Polymorphism percentage of all the tested genotypes of the mung bean and lentil was 100% and in faba bean, it ranged from 97 to 100%. Existence of genetic diversity of these tested mung bean, lentil and faba bean genotypes offers opportunities to exploit favourable alleles for use in the breeding program aimed at yield improvement.

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Section: Discussionmentioning

confidence: 99%

Genetic Diversity and Field Performance of Mung Bean, Faba Bean and Lentil Genotypes in the Kingdom of Saudi Arabia

Alghamdi

Al-Faifi

Migdadi

et al. 2017

IJAB

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“…Understanding and estimation of genetic diversity with plant species aids to select diverse parental lines to develop the segregating populations having more variability (McCouch et al, 2013). Although, efforts has been done to increase the production of chickpea through conventional breeding approaches (Ahmad et al, 2010); nonetheless, genetic improvement through the use of these recent approaches is quite slow, possibly due to existence of huge genotype × environment interactions for grain yield and related traits (Kumar and Ali, 2006). However, as indicated in this study, use of molecular markers offers new perspectives for improving the chickpea production in diverse environments (Varshney et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Field Performance and Genetic Diversity of Chickpea Genotypes

Khan¹,

Ammar²,

Migdadi³

et al. 2016

IJAB

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Chickpea (Cicer arietinum L.) is an important legume crop widely grown across the globe. In this study, twenty six chickpea genotypes, of diverse origin, were evaluated for field performance and genetic diversity. Significant differences in morphological characters of tested chickpea genotypes were observed at vegetative and reproductive stages under field conditions. Amplified fragments length polymorphism (AFLP) and sequence-related amplified polymorphism (SRAP) markers, used in this study, showed considerable genetic diversity among the tested genotypes. With markers, 716 AFLP and 1164 polymorphic SRAP loci were analyzed using four AFLP and six SRAP primer combinations. The values for polymorphism information content for SRAP and AFLP were more than 0.6 and 0.9, respectively. In addition, many subclusters within genotypes were noted, which indicated high diversity level in the tested genotypes. Clustering of chickpea genotypes was largely based on genetic background and/or origin. Both morphological and molecular data helped to quantify the genetic diversity in tested chickpea genotypes and may be useful for the use in breeding program aimed at yield improvement.

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“…In the present study, we acquired clear, stable and reproducible amplification bands with an optimized system and procedure (Wang et al, 2010). Therefore, RAPD markers still provide a rapid and useful technique to investigate genetic diversities at the whole genome level (Ahlawat et al, 2010;Ahmad et al, 2010;Leal et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

RAPD-based genetic diversities and correlation with morphological traits in Camellia (Theaceae) cultivars in China

Hy²,

Zheng³

et al. 2011

Genet. Mol. Res.

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ABSTRACT.Camellia is an economically important ornamental plant that has many uses, such as in beverages, foods and medicines. We examined 15 Camellia cultivars in Wenzhou, China, using RAPD markers and measurements of three traits (petal color, flower diameter, blooming period). PCR amplification with 15 random primers produced 1935 bands, observed at 88 amplification loci; 77% of the amplified loci were polymorphic, with a mean of 4.5 polymorphic loci per primer. The similarity coefficient ranged from 0.5419 to 0.7933 among the 15 samples; the lowest value was between Manao (C. reticulata) and Feibai FR (C. japonica), and the largest value was between Chidan (C. japonica) and Yuanyang FG (C. japonica). Cluster analysis divided the 15 cultivars into two groups at the similarity coefficient of 0.65. A correlation was found between RAPD markers and petal color in the first group. No correlation was found between RAPD markers and the other traits (flower diameter, blooming period). This study provides information useful for the identification, classification, phylogenesis, and breeding of Camellia cultivars.

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Genetic diversity of chickpea (Cicer arietinum L.) germplasm in Pakistan as revealed by RAPD analysis

Cited by 29 publications

References 13 publications

Genetic Diversity and Field Performance of Mung Bean, Faba Bean and Lentil Genotypes in the Kingdom of Saudi Arabia

Genetic Diversity and Field Performance of Mung Bean, Faba Bean and Lentil Genotypes in the Kingdom of Saudi Arabia

Field Performance and Genetic Diversity of Chickpea Genotypes

RAPD-based genetic diversities and correlation with morphological traits in Camellia (Theaceae) cultivars in China

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